FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108767
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGATAGCCGTGTCTGCGGCGGTGGTTGACCTCATTATCAGGGAGA38640.348495220366407No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38310.3455189413104827No Hit
ATCTATTACTGTGCGACTAGTGGGGCCTACGTAAGGGAGGGGTTTGACTA32650.29447124598766017No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28770.25947741951194436No Hit
GCCTTGGGCTGACCTAGGACGATCAGTTCGGTCCCTCCGCCGAATGCCACATGGGTGTAGGCGCCCTCATATGAG26410.23819251474836464No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25190.22718930126888695No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22480.20274773690053907No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22430.2022967855284293No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG21670.19544232467236128No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGTTCGGTCCCTC21090.1902112887558883No Hit
GATTATATCTGCTGCTCATATGAGGGCGCCTACACCCATGTGGCATTCGG20750.18714481942554206No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20490.18479987229057143No Hit
GACCTAGGACGATCAGTTCGGTCCCTCCGCCGAATGCCACATGGGTGTAG20270.18281568625328856No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19190.1730751366157182No Hit
ATCCTGGGGTCAGTCAGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG19170.17289475606687427No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18880.1702792381086378No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18680.16847543262019882No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18570.1674833396015574No Hit
CTTCTATACTGGGACCACCTTCTACAACCCGTCCCTCAGGAGTCGAGTCA18430.16622067575965013No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18320.1652285827410087No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18050.1627934453316161No Hit
GTACCAACACCACCCTGACAAAGCCCCCAAACTCATTATTTATGCAGTCA18020.16252287450835026No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17190.15503708173132857No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16780.1513392804800287No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16750.1510687096567629No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16360.1475512889543069No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16210.14619843483797768No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATATCTGCTGCTCATAT16150.145657293191446No Hit
GCTCATATGAGGGCGCCTACACCCATGTGGCATTCGGCGGAGGGACCGAACTGATCGTCCTAGGTCAGCCCAAGG16110.1452965320937582No Hit
GGGCACAGGATCCTGGGGTCAGTCAGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC15830.14277120440994365No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT15610.1407870183726608No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15460.1394341642563316No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15370.13862245178653407No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15190.136999026846939No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15120.13636769492598536No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14940.13474426998639027No Hit
GATATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGTAGG14860.1340227477910147No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAATTATGTCTCCTGGTACCAACACCACCCTGACAAAGC14720.13276008394910743No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14530.1310464687350904No Hit
GTCTCTGGTGGCTACATCAGCGGTAGTGATTATTACTGGACCTGGATCCG14170.12779961885590027No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14150.1276192383070564No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAATTATGTCTCCTGGTACCAACACCA14060.12680752583725888No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13930.12563505226977353No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13900.1253644814465077No Hit
CACCTGCACTGTCTCTGGTGGCTACATCAGCGGTAGTGATTATTACTGGA13850.12491353007439795No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13780.12428219815344432No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13600.12265877321384926No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC13580.12247839266500538No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCC13430.12112553854867615No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGGTCAGTCAGCCCTGACTCAGC12800.11544355126009342No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12630.11391031659492029No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAGCACGGCCTACCTGACCATCTCTGGGCTCCAGG12540.11309860412512278No Hit
CCCCTGAGGGCCGCTGATTGACTGCATAAATAATGAGTTTGGGGGCTTTGTCAGGGTGGTGTTGGTACCAGGAGA12470.11246727220416913No Hit
CATATGAGCAGCAGATATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAG12450.11228689165532522No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12400.1118359402832155No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12290.11084384726457407No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12280.11075365699015213No Hit
CCCATGTGGCATTCGGCGGAGGGACCGAACTGATCGTCCTAGGTCAGCCC12220.11021251534362043No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12090.10904004177613512No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11980.10804794875749368No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11920.10750680711096199No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11900.1073264265621181No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11850.10687547519000835No Hit
GGATGAGGCTGATTATATCTGCTGCTCATATGAGGGCGCCTACACCCATGTGGCATTCGGCGGAGGGACCGAA11740.10588338217136692No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11570.10435014750619381No Hit
GTGTTGGTACCAGGAGACATAATTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA11520.10389919613408409No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11470.10344824476197434No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11450.10326786421313044No Hit
GGTGTAGGCGCCCTCATATGAGCAGCAGATATAATCAGCCTCATCCTCAG11450.10326786421313044No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11390.10272672256659875No Hit
ACGTAAGGGAGGGGTTTGACTACTGGGGCCATGGACCCCTGGTCACCGTC11390.10272672256659875No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11200.10101310735258175No Hit
GTATAGAAGATACTTCCAATAAATTCTAGTCCCTTCCCTGGGGGCTGGCGGATCCAGGTCCAGTAATAATCACTA11160.10065234625489396No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11110.10020139488278422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)