FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108767
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35500.3201754741979153No Hit
GTAATAGATAGCCGTGTCTGCGGCGGTGGTTGACCTCATTATCAGGGAGA34230.3087213093463279No Hit
ATCTATTACTGTGCGACTAGTGGGGCCTACGTAAGGGAGGGGTTTGACTA31270.2820249881174314No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27530.2482938254836228No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27500.24802325466035693No Hit
GCCTTGGGCTGACCTAGGACGATCAGTTCGGTCCCTCCGCCGAATGCCACATGGGTGTAGGCGCCCTCATATGAG25990.2344045232226428No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24580.22168769452914813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23200.20924143665891934No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21560.19445023165371986No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG21060.18994071793262246No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20840.1879565318953396No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20470.1846194917417275No Hit
CTTCTATACTGGGACCACCTTCTACAACCCGTCCCTCAGGAGTCGAGTCA19580.17659255731817414No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGTTCGGTCCCTC19540.17623179622048635No Hit
GATTATATCTGCTGCTCATATGAGGGCGCCTACACCCATGTGGCATTCGG19430.17523970320184493No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19280.17388684908551572No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18950.17091057002959142No Hit
ATCCTGGGGTCAGTCAGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG18120.16342477725256974No Hit
GTACCAACACCACCCTGACAAAGCCCCCAAACTCATTATTTATGCAGTCA16950.15287251514520184No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16910.15251175404751405No Hit
GACCTAGGACGATCAGTTCGGTCCCTCCGCCGAATGCCACATGGGTGTAG16750.1510687096567629No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16420.1480924306008386No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16030.14457500989838262No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15890.14331234605647536No Hit
GGGCACAGGATCCTGGGGTCAGTCAGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC15750.14204968221456807No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATATCTGCTGCTCATAT15690.14150854056803638No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15570.14042625727497302No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAATTATGTCTCCTGGTACCAACACCACCCTGACAAAGC15130.1364578852004073No Hit
GCTCATATGAGGGCGCCTACACCCATGTGGCATTCGGCGGAGGGACCGAACTGATCGTCCTAGGTCAGCCCAAGG15120.13636769492598536No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT15040.13564617273060978No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15000.135285411632922No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14950.13483446026081222No Hit
GTCTCTGGTGGCTACATCAGCGGTAGTGATTATTACTGGACCTGGATCCG14840.1338423672421708No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14630.1319483714793099No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14550.13122684928393433No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14550.13122684928393433No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14000.12626638419072717No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13590.12256858293942731No Hit
CACCTGCACTGTCTCTGGTGGCTACATCAGCGGTAGTGATTATTACTGGA13580.12247839266500538No Hit
GATATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGTAGG13570.1223882023905834No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13530.12202744129289561No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13510.12184706074405173No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13270.11968249415792497No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13270.11968249415792497No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCC13260.11959230388350302No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAATTATGTCTCCTGGTACCAACACCA13260.11959230388350302No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGGTCAGTCAGCCCTGACTCAGC13230.11932173306023718No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC13200.11905116223697135No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13060.11778849839506407No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12970.11697678592526653No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12880.116165073455469No Hit
CCCCTGAGGGCCGCTGATTGACTGCATAAATAATGAGTTTGGGGGCTTTGTCAGGGTGGTGTTGGTACCAGGAGA12570.11336917494838862No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12290.11084384726457407No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12260.11057327644130822No Hit
GTGTTGGTACCAGGAGACATAATTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA11830.10669509464116447No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11800.10642452381789862No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11780.10624414326905471No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11550.10416976695734992No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11450.10326786421313044No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11360.1024561517433329No Hit
GGATGAGGCTGATTATATCTGCTGCTCATATGAGGGCGCCTACACCCATGTGGCATTCGGCGGAGGGACCGAA11330.10218558092006706No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11320.10209539064564511No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11200.10101310735258175No Hit
CATATGAGCAGCAGATATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAG11180.10083272680373784No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11150.10056215598047201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAA9450.058.93342670
TCTAGTA200.006087187754.2930266
GTACTAG306.6257856E-445.5483441
GGTATCA12850.044.3963431
GTGGTAT3700.043.394031
TGGTATC3850.039.9089282
CTACGGA9800.038.31011269
GTCACCG11900.036.08680370
GTATCAA31900.035.446321
GAGCTAG601.4363446E-534.161261
CGGGATA501.9782383E-434.14430617
TACTGTC601.4430172E-534.1381457
TCTACGG10650.033.38595668
GTCTACG10650.032.34462467
GACAGGT2350.031.9807531
TATCAAC36250.031.083092
GTGTCTA10950.030.89449565
CGAGCTC450.004718762430.53786735
ATCAACG36900.030.3450163
TGTCTAC11250.030.24322366
Produced by FastQC (version 0.11.5)