FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823967
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTAAAGTCTTCGG63320.7684773783416083No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG62710.7610741692325057No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT44660.5420119980533201No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40360.48982544203833406No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC36950.44844028947761255No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34040.41312334110468013No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC32730.39722464613267283No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32370.3928555391174647No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC29370.35644631399073023No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC28850.35013538163542957No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26170.31760980718888016No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC24640.2990411023742456No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTTACCACTCAGGCTGTCATCCCATG24520.2975847333691762No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT24450.2967351847828857No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23520.28544832499359807No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG23510.2853269609098423No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTTACCACTCAGG23430.28435604823979604No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23300.28277831515097085No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC22830.2770742032144491No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22550.2736760088692872No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22200.26942826593783487No Hit
GATTATCCCTATAGACGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG22080.2679718969327655No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20720.25146638154197926No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTTACCACTCAGGC20120.24418453651663236No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC19510.23678132740752966No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC19300.23423268164865824No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19130.23216949222480998No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18340.2225817296081032No Hit
GGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG18260.221610816938057No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17980.2182126225928951No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17860.21675625358782571No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17660.21432897191271008No Hit
CCCCTGAGGGCCGCTGATTATCCCTATAGACGAGGAGTTTGGGGGCCGTT17340.21044532123252507No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17230.2091103163112115No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT16170.19624572343309865No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15660.1900561551615538No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15610.1894493347427749No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15580.18908524249150754No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT15420.18714341715141505No Hit
GTATAGATAATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA15240.18495886364381098No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG14830.17998293620982395No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG14490.17585655736212735No Hit
GCATGGGATGACAGCCTGAGTGGTAAAGTCTTCGGAACTGGGACCAAGGT14200.17233699893320972No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC14080.17088062992814032No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14040.17039517359311718No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14030.17027380950936144No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG13820.16772516375049001No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13770.1671183433317111No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13380.1623851440652356No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATCTATACTGGTACCAGCAGCTCCCAGGAACGGCCCC13060.1585014933850506No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC12890.1564383039612023No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12880.15631693987744655No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC12700.1541323863698425No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12650.15352556595106356No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTAAAGTCTTCGGAACTGGGACC12560.15243328919726154No Hit
CTTTACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGG12440.15097692019219217No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12330.14964191527087856No Hit
GGATGACAGCCTGAGTGGTAAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC12290.14915645893585544No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12190.14794281809829762No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12170.14770008993078607No Hit
GTGGTAAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC12070.14648644909322825No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGAAGATACA12030.14600099275820513No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12010.14575826459069355No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11640.14126779349172963No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11590.14066097307295072No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG11530.13993278857041605No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11470.13920460406788138No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11280.1368986864765215No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT11050.13410731255013855No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10940.13277230762882494No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTAAAGTCTTCGGAACTGGGAC10860.13180139495877868No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10840.13155866679126713No Hit
TATCTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCGT10760.13058775412122087No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG10710.129980933702442No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATAGATAATTACTT10680.1296168414511746No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA10630.12901002103239573No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT10620.12888865694863993No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC10560.12816047244610526No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATG10440.12670410344103586No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10410.1263400111897685No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10220.12403409359840868No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG10070.12221363234207194No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG10040.1218495400908046No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATCCCTATAGACGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTA10030.12172817600704883No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG10000.12136408375578148No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG9990.1212427196720257No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG9990.1212427196720257No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG9870.11978635066695632No Hit
CCCAGGAACGGCCCCCAAACTCCTCGTCTATAGGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG9810.11905816616442164No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT9770.1185727098293985No Hit
ATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCGTCTATAGGGATAATCAGCGGCCCTCAGGGGT9600.11650952040555022No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC9520.11553860773550396No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG9500.11529587956799241No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG9460.11481042323296929No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA9390.11396087464667883No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG9350.11347541831165568No Hit
GCTGGTACCAGTATAGATAATTACTTCCGATGTTGGAGCTGCTTCCAGAA9300.11286859789287679No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG9250.11226177747409787No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG9170.1112908648040516No Hit
GCCCCCAAACTCCTCGTCTATAGGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT9070.11007722396649379No Hit
GCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG9040.10971313171522647No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG9030.10959176763147067No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTGGTAAAGTCTTCGGAACTG8960.10874221904518021No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT8940.10849949087766864No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8920.10825676271015708No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT8870.10764994229137817No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC8810.1069217577888435No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC8800.1068003937050877No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG8770.10643630145382035No Hit
CGTCTATAGGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC8660.10510129653250676No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC8590.10425174794621629No Hit
GGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTAAAGTCTTCGGAACTGGGACCAAGGT8580.1041303838624605No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8550.10376629161119318No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGG8480.10291674302490271No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8430.10230992260612379No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTAT8430.10230992260612379No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC8400.10194583035485645No Hit
CTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAG8390.10182446627110067No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA8380.10170310218734488No Hit
GGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGT8310.10085355360105441No Hit
CCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGAT8310.10085355360105441No Hit
GTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCC8290.10061082543354286No Hit
GCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCT8240.10000400501476395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT100.00786162140.0701970
GTGGTAT2250.054.654241
CGCGTAT200.00740098851.67429439
TATATAG200.007653104651.2383462
TTGACCG200.007653104651.2383463
TGGTATC2500.047.822462
GGTATCA7800.045.1072651
CGTATGC1000.041.4129541
GCCAGAG24850.040.58371470
TCGTATG856.730261E-1140.56859240
ATGCCGT1150.039.12736544
ACTACTT350.001410828639.0624525
TTGATTA451.0616472E-437.954333
CTTGATT451.0616472E-437.954332
CGGGTAC451.0627859E-437.94742217
ACGAGAC1450.037.6971522
GAGCGAT1200.037.10004832
GTATCAA18750.036.98271
TGCTTGA1151.8189894E-1236.62436355
GTATGCC1151.8189894E-1236.05105642
Produced by FastQC (version 0.11.5)