FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004687779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880464
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33480.3802540478656708No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33420.3795725889985281No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTGTCAGGGAGA24990.28382761816496754No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23390.26565538170782677No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20530.23317250904068762No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20270.23021952061640225No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19910.2261307674135456No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGTTATTAGCAGCTGGTTAGCCTGGTATCAGGTAAAA19850.2254493085464028No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19090.21681749622926094No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18930.21500027258354684No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18760.21306947245997562No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17990.20432408366497665No Hit
GTGTATTACTGTGCGAGAGAATATTGTAGTAATGGTTTTTGCCATACGGC16950.19251212996783515No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16640.18899125915426412No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16590.18842337676497845No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16520.18762834141997856No Hit
ATGCTACATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCCGGGACAGATTTCACTCTCA16270.1847889294735503No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15950.18115448218212216No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15900.1805865997928365No Hit
GGCTAACATTTTCCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15760.1789965291028367No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15630.177520034890694No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACAGGCTAACATTTTC14680.16673026949426667No Hit
GGTTATTAGCAGCTGGTTAGCCTGGTATCAGGTAAAACCAGGGAGAGCCCCTAAACTCCTGATCTATGCTACATC14450.16411801050355268No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14370.16320939868069562No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14120.1603699867342674No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14060.15968852786712462No Hit
CTCCTGATCTATGCTACATCCAGTTTACAAAGTGGGGTCCCATCAAGGTT14010.15912064547783897No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13890.1577577277435534No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13440.15264678623998254No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT13280.1508295625942685No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13200.14992095077141143No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13190.1498073742935543No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12340.14015337367569827No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12180.1383361500299842No Hit
GGTAAAACCAGGGAGAGCCCCTAAACTCCTGATCTATGCTACATCCAGTTTACAAAGTGGGGTCCCATCAAGGTT12130.13776826764069855No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11890.1350424321721274No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11800.13402024387141326No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11670.13254374965927057No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC11410.12959076123498517No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTGTCAGGGAGAACTGGTTCTTGGACGTGTCGA11260.12788711406712824No Hit
ATGTTAGCCTGTTGACAAAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC11190.12709207872212835No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGCGGCAGCTCCCAGATGGGTC11130.12641061985498556No Hit
GTCCGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACAGG11050.12550200803212852No Hit
GATCAGGAGTTTAGGGGCTCTCCCTGGTTTTACCTGATACCAGGCTAACC11010.1250477021207No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10790.12254901960784313No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG10710.12164040778498611No Hit
GTATTACTGTGCGAGAGAATATTGTAGTAATGGTTTTTGCCATACGGCCATTAACTACTATTACTACGGTCTGGA10690.12141325482927184No Hit
GGCCAAAAGTGGGAGGGAAAATGTTAGCCTGTTGACAAAAGTAAGTTGCA10670.12118610187355759No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10550.11982318413927201No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGTTATTAGCAG10390.11800596049355795No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10300.11698377219284378No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10110.11482581911355831No Hit
GTTTCTGGTTACTCCATCAGCAGTGGTTTCTACTGGGTCTGGATCCGGCG10060.11425793672427266No Hit
CCCCTAAACTCCTGATCTATGCTACATCCAGTTTACAAAGTGGGGTCCCA9980.11334932490141562No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAAC9940.11289501898998709No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9790.11119137182213014No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9650.10960130113213033No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9580.10880626578713042No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9490.10778407748641625No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9460.10744334805284488No Hit
GTGTCAGGGAGAACTGGTTCTTGGACGTGTCGACTGATATGGTGACTCGA9420.10698904214141636No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9400.10676188918570208No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9310.10573970088498792No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9220.10471751258427375No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9200.10449035962855949No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9190.10437678315070235No Hit
GATCTATGCTACATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCCGGGACAGATTTCA9190.10437678315070235No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9160.10403605371713098No Hit
GTTTAGGGGCTCTCCCTGGTTTTACCTGATACCAGGCTAACCAGCTGCTA9130.10369532428355958No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9120.10358174780570244No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9100.10335459484998819No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9020.10244598302713115No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8940.10153737120427411No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8930.10142379472641698No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8900.1010830652928456No Hit
CATCAAGGTTCAGCGGCAGTGGGTCCGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG8820.10017445346998854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)