FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880464
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32190.36560268222210107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26300.29870613676425156No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24270.27565011175925425No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTGTCAGGGAGA22810.25906794599211325No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21800.24759672172854313No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19110.21704464918497518No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18970.2154545784949754No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18810.21363735484926127No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17620.20012175398426285No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGTTATTAGCAGCTGGTTAGCCTGGTATCAGGTAAAA17570.1995538715949772No Hit
GTGTATTACTGTGCGAGAGAATATTGTAGTAATGGTTTTTGCCATACGGC17450.19819095386069163No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17250.19591942430354903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17190.19523796543640626No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16170.18365316469497903No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16110.18297170582783623No Hit
ATGCTACATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCCGGGACAGATTTCACTCTCA15940.181040905704265No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15630.177520034890694No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15410.17502135237783714No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15150.17206836395355177No Hit
CTCCTGATCTATGCTACATCCAGTTTACAAAGTGGGGTCCCATCAAGGTT15120.17172763451998035No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14930.1695696814406949No Hit
GGCTAACATTTTCCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14770.16775245779498082No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14550.16525377528212398No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14410.16366370459212415No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13900.15787130422141052No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13840.15718984535426775No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACAGGCTAACATTTTC13740.15605408057569645No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13580.15423685692998237No Hit
GGTTATTAGCAGCTGGTTAGCCTGGTATCAGGTAAAACCAGGGAGAGCCCCTAAACTCCTGATCTATGCTACATC13360.1517381744171255No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13150.14935306838212578No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12740.14469643278998348No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12610.14321993857784077No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT12590.14299278562212653No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12570.14276563266641226No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11980.1360646204728416No Hit
ATGTTAGCCTGTTGACAAAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC11880.1349288556942703No Hit
GTCCGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACAGG11690.1327709026149848No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11600.13174871431427065No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11590.13163513783641353No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10990.12482054916498574No Hit
GGCCAAAAGTGGGAGGGAAAATGTTAGCCTGTTGACAAAAGTAAGTTGCA10970.12459339620927148No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10770.12232186665212888No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10640.12084537243998619No Hit
GGTAAAACCAGGGAGAGCCCCTAAACTCCTGATCTATGCTACATCCAGTTTACAAAGTGGGGTCCCATCAAGGTT10570.12005033709498628No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTGTCAGGGAGAACTGGTTCTTGGACGTGTCGA10360.11766523105998655No Hit
GTATTACTGTGCGAGAGAATATTGTAGTAATGGTTTTTGCCATACGGCCATTAACTACTATTACTACGGTCTGGA10230.11618873684784387No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGTTATTAGCAG10190.11573443093641535No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10140.1151665485471297No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGCGGCAGCTCCCAGATGGGTC10140.1151665485471297No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10100.11471224263570118No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9980.11334932490141562No Hit
GAGTGAAATCTGTCCCGGACCCACTGCCGCTGAACCTTGATGGGACCCCA9960.11312217194570137No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC9920.11266786603427283No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9740.1106234894328445No Hit
GATCAGGAGTTTAGGGGCTCTCCCTGGTTTTACCTGATACCAGGCTAACC9680.10994203056570173No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9460.10744334805284488No Hit
GTTTCTGGTTACTCCATCAGCAGTGGTTTCTACTGGGTCTGGATCCGGCG9430.10710261861927348No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9430.10710261861927348No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9400.10676188918570208No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAAC9380.10653473622998781No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9320.10585327736284504No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9300.10562612440713079No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9250.10505824201784512No Hit
GTGTCAGGGAGAACTGGTTCTTGGACGTGTCGACTGATATGGTGACTCGA9180.10426320667284524No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9140.10380890076141672No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9100.10335459484998819No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG9080.10312744189427392No Hit
GTATCATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTCGACACGTCCAAGAA9080.10312744189427392No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC9050.10278671246070255No Hit
CCCCTAAACTCCTGATCTATGCTACATCCAGTTTACAAAGTGGGGTCCCA8970.1018781006378455No Hit
GTTTAGGGGCTCTCCCTGGTTTTACCTGATACCAGGCTAACCAGCTGCTA8950.10165094768213123No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA8940.10153737120427411No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8820.10017445346998854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAA200.007787876851.0128719
GCTAGAC200.007800130650.9925843
GTCTTAT350.001442876138.884631
CTAGTAC350.001448933738.8514983
AGGTATC451.09218716E-437.7722853
GTGGTAT2800.036.4543381
TAGCGCG400.002733579434.13855429
GTATAAT701.084949E-634.0240481
CGTACCA1600.033.98733514
TACGTAC1600.033.98733512
GTATCAA22050.033.9468961
GACTGAT7450.033.69759470
GAATACG1650.032.959299
ATACGTA1650.032.9592911
ACGTACC1650.032.95741713
AATACGT1750.031.075910
GTGCTAG1900.030.4425681
TATAACC450.0049677330.2178293
TGTTAGA10600.029.91623570
ACGGGTA1052.6828275E-829.14524316
Produced by FastQC (version 0.11.5)