FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151586
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCCTCAGAGTCAGGGACCC63290.5495898699706318No Hit
GTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCCTCAGAGTCA54840.4762128056436949No Hit
GTAGTAATTACCCTCATCCTCAGCCTGGAGTCCACTGATGGCCAGGGAGG51340.44581993876271503No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49010.4255869730962343No Hit
ACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC48850.4241975848959609No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48340.41976891000758954No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47750.41464554101908146No Hit
AATTACTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGG47590.4132561528188082No Hit
GACTTAGGACGGTCAGCTTGGTCCCGCCGCCGAATATCACACCATTCAGA43350.3764373655115641No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG43210.37522165083632486No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCGC42600.3699246083227827No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41310.35872266595807867No Hit
GTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTG36860.3200803066379758No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34710.3014104026968025No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG34440.2990658101088412No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCGCCGCCGAATATCACACCATTCAGAATGTCATCCCATG33100.28742968393155177No Hit
ATATCACACCATTCAGAATGTCATCCCATGCTGCACAGTAGTAATTACCC32750.2843903972434538No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG32600.2830878458056975No Hit
GTCTGGGGGCCGCTCCTGGGACTTGGTGGTACCAGTTCACAATATTGTTT32540.28256682523059506No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32420.28152478408039No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32040.27822498710474075No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCAC31880.2768355989044674No Hit
GTCCCAGGAGCGGCCCCCAGACTCCTCATTTATGATAACAATCAGCGGCCCTCAGGGGTCCCTGACCGAGTCT31820.2763145783293649No Hit
GTGTGATATTCGGCGGCGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC31260.2714517196284081No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31140.27040967847820313No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30600.26572049330228054No Hit
GCCCCCAGACTCCTCATTTATGATAACAATCAGCGGCCCTCAGGGGTCCCTGACCGAGTCTCTGGCTCCAAGT29310.2545185509375765No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT28910.2510450804368931No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28540.24783212022376097No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG28350.24618222173593635No Hit
CTCCTGGGACTTGGTGGTACCAGTTCACAATATTGTTTCCGATGTTGGAACTGCTTCCAGAACAAGAGATGGTGA28190.244792833535663No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCAGTTCCAACATC27740.24088517922239414No Hit
CCCCTGAGGGCCGCTGATTGTTATCATAAATGAGGAGTCTGGGGGCCGCTCCTGGGACTTGGTGGTACCAGTTCA27160.2358486469964032No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCCTCAGAGTCAGGGA27130.23558813670885195No Hit
CCTCAGGGGTCCCTGACCGAGTCTCTGGCTCCAAGTCTGGCACGTCAGCCTCCCTGGCCATCAGTGGACTCCAGG27030.2347197690836811No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26780.232548850020754No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25400.22056537679339622No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25400.22056537679339622No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25260.21934966211815707No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24820.21552884456740531No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24400.21188170054168773No Hit
CCCTCAGAGTCAGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG23640.20528210659038923No Hit
GTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCGGCGGGACC23630.20519526982787217No Hit
GTCCCTGACCGAGTCTCTGGCTCCAAGTCTGGCACGTCAGCCTCCCTGGC22870.1985956758765737No Hit
ATGCTGCACAGTAGTAATTACCCTCATCCTCAGCCTGGAGTCCACTGATG22860.1985088391140566No Hit
GGGTAGAGAAGACAGGACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCA22820.19816149206398828No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22630.19651159357616366No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22280.1934723068880657No Hit
ATTCAGAATGTCATCCCATGCTGCACAGTAGTAATTACCCTCATCCTCAG21990.1909540407750702No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20770.18035995574798583No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20310.1763654646721999No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20240.1757576073345803No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20090.17445505589682403No Hit
CTGCTGACTCAGCCACCCTCAGAGTCAGGGACCCCCGGACAGAGGGTCAC20080.17436821913430695No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20030.17393403532172153No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTGTTATCATAAATGAGGAGTCTGGGGGCCGCTCCTGGGACTTGGTGGTA19960.17332617798410194No Hit
CTCTTGTTCTGGAAGCAGTTCCAACATCGGAAACAATATTGTGAACTGGTACCACCAAGTCCCAGGAGCGGCCCC19400.16846331928314515No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAGTCTCCAGCATGGCCAG19360.1681159722330768No Hit
CCCCCAGACTCCTCATTTATGATAACAATCAGCGGCCCTCAGGGGTCCCT18230.1583034180686462No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18220.15821658130612912No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18040.15665351958082158No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17880.15526413138054823No Hit
AGACAGGACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT17800.15456943728041153No Hit
TCAGTGGACTCCAGGCTGAGGATGAGGGTAATTACTACTGTGCAGCATGG17400.15109596677972814No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCGCCGCCGAATATCACAC17400.15109596677972814No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17210.14944606829190352No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17140.1488382109542839No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17080.1483171903791814No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTC17060.14814351685414723No Hit
GCATGGGATGACATTCTGAATGGTGTGATATTCGGCGGCGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGG16910.14684096541639097No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16540.1436280052032588No Hit
GGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16440.14275963757808796No Hit
GCCCTCAGGGGTCCCTGACCGAGTCTCTGGCTCCAAGTCTGGCACGTCAG16430.14267280081557088No Hit
GGGTAATTACTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCGGCGGGACCAAGCTGACCGT16280.1413702493778146No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16220.14084922880271208No Hit
TTACTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCG15950.1385046362147508No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTC15900.13807045240216537No Hit
GCACAGTAGTAATTACCCTCATCCTCAGCCTGGAGTCCACTGATGGCCAGGGAGGCTGACGTGCCAGACTTGGAG15850.13763626858957992No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15800.1372020847769945No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15750.13676790096440908No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15740.136681064201892No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15690.13624688038930657No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCA15590.13537851276413573No Hit
CTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTG15260.1325128996010719No Hit
ATCCCATGCTGCACAGTAGTAATTACCCTCATCCTCAGCCTGGAGTCCAC15260.1325128996010719No Hit
GTCTCTGCTGACTCAGCCACCCTCAGAGTCAGGGACCCCCGGACAGAGGG15170.13173136873841815No Hit
GTCAGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCAGTTCCAACATCGGAAACAATATTGTGAA15090.13103667463828145No Hit
GTTCCAACATCGGAAACAATATTGTGAACTGGTACCACCAAGTCCCAGGA15050.13068932758821314No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCCTCAGAGTCAGGGACCCCCGGACAGAGGGTCAC15040.13060249082569603No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15020.13042881730066186No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14900.12938677615045685No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14810.1286052452878031No Hit
ATTCTGAATGGTGTGATATTCGGCGGCGGGACCAAGCTGACCGTCCTAAG14770.12825789823773473No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14610.12686851003746138No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14590.12669483651242722No Hit
GGATGACATTCTGAATGGTGTGATATTCGGCGGCGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC14530.12617381593732468No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14420.12521861154963676No Hit
CTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCGGCG14420.12521861154963676No Hit
ATTGTGAACTGGTACCACCAAGTCCCAGGAGCGGCCCCCAGACTCCTCAT14310.12426340716194882No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG14150.12287401896167546No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13980.12139779399888502No Hit
ACTCCAGGCTGAGGATGAGGGTAATTACTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCGG13730.11922687493595789No Hit
GAGTCAGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCAG13610.11818483378575287No Hit
GCCTGGAGTCCACTGATGGCCAGGGAGGCTGACGTGCCAGACTTGGAGCC13540.11757697644813328No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG13190.11453768976003528No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13110.11384299565989861No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13090.11366932213486443No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT12920.11219309717207399No Hit
ATGTCATCCCATGCTGCACAGTAGTAATTACCCTCATCCTCAGCCTGGAG12850.11158523983445441No Hit
CAGTAGTAATTACCCTCATCCTCAGCCTGGAGTCCACTGATGGCCAGGGA12810.11123789278438606No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC12780.11097738249683481No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG12670.11002217810914686No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12240.10628819732091221No Hit
GGCCATCAGTGGACTCCAGGCTGAGGATGAGGGTAATTACTACTGTGCAG12200.10594085027084386No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12160.10559350322077551No Hit
GGATGAGGGTAATTACTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCGGCGGGACCAAG12110.1051593194081901No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11990.10411727825798507No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11890.10324891063281422No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC11860.10298840034526296No Hit
GTCCACTGATGGCCAGGGAGGCTGACGTGCCAGACTTGGAGCCAGAGACTCGGTCAGGGACCCCTGAGGGCCG11850.1029015635827459No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT11690.10151217538247252No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11640.1010779915698871No Hit
GAGGGTCACCATCTCTTGTTCTGGAAGCAGTTCCAACATCGGAAACAATA11630.10099115480737002No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11610.10081748128233585No Hit
CCCTCAGAGTCAGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCAGTTCCAACATCGGAAACAAT11600.10073064451981875No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT11580.1005569709947846No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCAAATCTGT11560.10038329746975042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAC150.00224122769.87102552
TACGCTG150.002366828768.9168937
TATCTAT200.00612365654.21126667
GTACGCT200.007413328651.65386636
GGTATCA6600.051.44081
TCGTATG404.966795E-543.17666240
GTGGTAT1400.041.6425481
GTATCAA20000.040.295291
TCTCGTA350.001351759339.40514438
TATACAC801.7407729E-938.5805973
TATCAAC22250.036.2204862
TGGTACG400.002067068436.14084667
GTTGCCA750.002218966835.61968670
TCAACGC23550.034.803544
ATCAACG23500.034.731663
CGTATGC400.00257283834.56400341
GCGCTAC501.8915292E-434.4059134
CAACGCA23750.034.3660625
CTTATAC601.3987912E-534.2938651
AACGCAG24100.033.7246746
Produced by FastQC (version 0.11.5)