FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151586
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCCTCAGAGTCAGGGACCC64500.560097118235199No Hit
GACTTAGGACGGTCAGCTTGGTCCCGCCGCCGAATATCACACCATTCAGA52440.45537198263959444No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51610.44816453135067635No Hit
GTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCCTCAGAGTCA51090.4436490196997879No Hit
GTAGTAATTACCCTCATCCTCAGCCTGGAGTCCACTGATGGCCAGGGAGG48480.42098462468282877No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45040.3911127783769514No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44530.3866841034885801No Hit
ACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC43780.3801713462997987No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCGCCGCCGAATATCACACCATTCAGAATGTCATCCCATG43200.3751348140738078No Hit
AATTACTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGG42010.36480123933427466No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41120.35707276747025407No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCGC41020.3562043998450832No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39220.34057378259200793No Hit
GTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTG38830.33718714885384155No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG35390.3073153025479643No Hit
GTGTGATATTCGGCGGCGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC33870.29411611464536735No Hit
GTCTGGGGGCCGCTCCTGGGACTTGGTGGTACCAGTTCACAATATTGTTT33070.2871691736440005No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32910.2857797854437272No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG32440.28169845760542417No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32160.2792670282549458No Hit
ATATCACACCATTCAGAATGTCATCCCATGCTGCACAGTAGTAATTACCC31490.2734489651663011No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30160.2618996757515288No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30030.2607707978388067No Hit
GTCCCAGGAGCGGCCCCCAGACTCCTCATTTATGATAACAATCAGCGGCCCTCAGGGGTCCCTGACCGAGTCT29860.2592945728760162No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29560.2566894700005037No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29450.2557342656128157No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCAC29390.2552132450377132No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28570.24809263051131222No Hit
CTCCTGGGACTTGGTGGTACCAGTTCACAATATTGTTTCCGATGTTGGAACTGCTTCCAGAACAAGAGATGGTGA28380.2464427320234876No Hit
GCCCCCAGACTCCTCATTTATGATAACAATCAGCGGCCCTCAGGGGTCCCTGACCGAGTCTCTGGCTCCAAGT27220.23636966757150574No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26290.2282938486574168No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25960.225428235494353No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25930.22516772520680176No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25610.222388948806255No Hit
CCCCTGAGGGCCGCTGATTGTTATCATAAATGAGGAGTCTGGGGGCCGCTCCTGGGACTTGGTGGTACCAGTTCA25470.2211732341310158No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCCTCAGAGTCAGGGA24350.21144751672910228No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23950.2079740462284189No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23860.20719251536576513No Hit
GGGTAGAGAAGACAGGACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCA23760.2063241477405943No Hit
GTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCGGCGGGACC23610.20502159630283798No Hit
CCTCAGGGGTCCCTGACCGAGTCTCTGGCTCCAAGTCTGGCACGTCAGCCTCCCTGGCCATCAGTGGACTCCAGG23170.20120077875208625No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22740.1974667979638516No Hit
ATGCTGCACAGTAGTAATTACCCTCATCCTCAGCCTGGAGTCCACTGATG22170.19251710250037773No Hit
ATTCAGAATGTCATCCCATGCTGCACAGTAGTAATTACCCTCATCCTCAG22110.19199608192527523No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21230.18435444682377175No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21210.18418077329873755No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21000.18235720128587876No Hit
GTCCCTGACCGAGTCTCTGGCTCCAAGTCTGGCACGTCAGCCTCCCTGGC20840.18096781308560542No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20600.17888373078519537No Hit
CTGCTGACTCAGCCACCCTCAGAGTCAGGGACCCCCGGACAGAGGGTCAC20440.17749434258492203No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20170.1751497499969607No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCAGTTCCAACATC19760.17158944273376023No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAGTCTCCAGCATGGCCAG19570.1699395442459356No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19410.16855015604566223No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19220.16690025755783763No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTGTTATCATAAATGAGGAGTCTGGGGGCCGCTCCTGGGACTTGGTGGTA19190.16663974727028635No Hit
CTCTTGTTCTGGAAGCAGTTCCAACATCGGAAACAATATTGTGAACTGGTACCACCAAGTCCCAGGAGCGGCCCC18930.16438199144484217No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18670.16212423561939796No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18470.16038750036905625No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCGCCGCCGAATATCACAC18260.15856392835619745No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTC18180.15786923425606078No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18090.157087703393407No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18060.15682719310585574No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18020.1564798460557874No Hit
TCAGTGGACTCCAGGCTGAGGATGAGGGTAATTACTACTGTGCAGCATGG17720.15387474318027486No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17660.15335372260517235No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17560.15248535498000151No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16510.14336749491570758No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCA16490.14319382139067338No Hit
GGGTAATTACTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCGGCGGGACCAAGCTGACCGT16430.14267280081557088No Hit
GGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16390.14232545376550254No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16170.14041504499012666No Hit
CCCTCAGAGTCAGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG15860.13772310535209703No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGT15730.13659422743937494No Hit
GTCTCTGCTGACTCAGCCACCCTCAGAGTCAGGGACCCCCGGACAGAGGG15660.13598637010175532No Hit
AGACAGGACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT15450.13416279808889653No Hit
GGATGACATTCTGAATGGTGTGATATTCGGCGGCGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC15330.1331207569386915No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTC15330.1331207569386915No Hit
GCATGGGATGACATTCTGAATGGTGTGATATTCGGCGGCGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGG15300.13286024665114027No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15220.13216555255100357No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTCTGCTGACTCAGCCACCCTCAGAGTCAGGGACCCCCGGACAGAGGGTCAC15160.13164453197590106No Hit
GTTCCAACATCGGAAACAATATTGTGAACTGGTACCACCAAGTCCCAGGA15120.13129718492583273No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCGCCGCCGAATAT15040.13060249082569603No Hit
CCCCCAGACTCCTCATTTATGATAACAATCAGCGGCCCTCAGGGGTCCCT14970.12999463348807644No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG14700.12765004090011514No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC14650.12721585708752972No Hit
GCACAGTAGTAATTACCCTCATCCTCAGCCTGGAGTCCACTGATGGCCAGGGAGGCTGACGTGCCAGACTTGGAG14510.12600014241229054No Hit
TTACTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCG14500.12591330564977343No Hit
CCCTCAGAGTCAGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCAGTTCCAACATCGGAAACAAT14470.12565279536222218No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14360.12469759097453424No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14320.12435024392446592No Hit
ATCCCATGCTGCACAGTAGTAATTACCCTCATCCTCAGCCTGGAGTCCAC14200.1233082027742609No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14140.12278718219915837No Hit
CTCCAGCATGGCCAGCTTCCCGCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTCTG14140.12278718219915837No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14120.12261350867412421No Hit
GCCCTCAGGGGTCCCTGACCGAGTCTCTGGCTCCAAGTCTGGCACGTCAG14070.12217932486153879No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC13800.11983473227357748No Hit
GTCAGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCAGTTCCAACATCGGAAACAATATTGTGAA13780.11966105874854333No Hit
GAGTCAGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCAG13690.11887952788588954No Hit
ATTCTGAATGGTGTGATATTCGGCGGCGGGACCAAGCTGACCGTCCTAAG13650.1185321808358212No Hit
GCCTGGAGTCCACTGATGGCCAGGGAGGCTGACGTGCCAGACTTGGAGCC13550.11766381321065035No Hit
GCTTGGTCCCGCCGCCGAATATCACACCATTCAGAATGTCATCCCATGCTGCACAGTAGTAATTACCCTCATCCT13520.1174033029230991No Hit
CTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCGGCG13350.11592707796030866No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13300.11549289414772322No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13250.1150587103351378No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13180.1144508529975182No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13130.11401666918493278No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12890.11193258688452273No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12770.11089054573431771No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12640.1097616678215956No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12570.10915381048397602No Hit
GGATGAGGGTAATTACTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCGGCGGGACCAAG12500.10854595314635641No Hit
ACTCCAGGCTGAGGATGAGGGTAATTACTACTGTGCAGCATGGGATGACATTCTGAATGGTGTGATATTCGGCGG12460.10819860609628809No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG12380.10750391199615139No Hit
ATGTCATCCCATGCTGCACAGTAGTAATTACCCTCATCCTCAGCCTGGAG12370.10741707523363431No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12260.10646187084594637No Hit
GAGGGTCACCATCTCTTGTTCTGGAAGCAGTTCCAACATCGGAAACAATA12090.10498564588315591No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12070.10481197235812176No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12060.10472513559560467No Hit
GTCCACTGATGGCCAGGGAGGCTGACGTGCCAGACTTGGAGCCAGAGACTCGGTCAGGGACCCCTGAGGGCCG12050.10463829883308759No Hit
ATTGTGAACTGGTACCACCAAGTCCCAGGAGCGGCCCCCAGACTCCTCAT11980.10403044149546799No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT11980.10403044149546799No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAGTCTCCAGCATGGCCAGCTTCCCGCTCCTC11750.10203319595757503No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGTTCCAACATCGGAAACAATATT11560.10038329746975042No Hit
CAGTAGTAATTACCCTCATCCTCAGCCTGGAGTCCACTGATGGCCAGGGA11550.10029646070723332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.049.284611
GTGGTAT1950.045.6860541
TGGTATC2200.040.4874232
GTATCAA19550.038.558561
ATACGTA1009.094947E-1237.7139311
TACGTAC1051.8189894E-1135.9211512
GAATACG1051.8189894E-1135.911789
TATCAAC20950.035.812082
GCTTGAC501.9378494E-434.264541
GTGCTAG1103.092282E-1134.2645381
ATGGCAA1806.539267E-933.6865970
ATCAACG22700.033.0454983
TCAACGC22700.033.0454984
CAACGCA23150.032.553935
AACGCAG23500.032.2232486
ACGTACC1208.367351E-1131.43100513
AATACGT1208.367351E-1131.42690710
CGCAGAG24450.031.1248828
AGAGTAC24450.030.99006511
GGAGCAT1550.030.9378663
Produced by FastQC (version 0.11.5)