FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218089
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGGCTCACAGAGA56300.46219939593904874No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT35210.28905933802866624No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA33470.2747746675325038No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG33140.2720655058866799No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC31530.25884808088735717No Hit
GTCTATTACTGTGCGAAACGATATAACAGTGGCTGGTACTGGTTTTTTGA30190.2478472426891631No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG28100.23068921893227837No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28000.22986826085778625No Hit
GATTATTACTGCACCTCATATGCAGGCAACAACAGGGATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT27700.22740538663430995No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26930.22108400946072082No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26870.2205914346160256No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25050.20564999766026948No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCCTGTTGTTGCCTGCATATGAGGTGCAGTAATAAT24540.20146311148035984No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT23680.1944028720397278No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23310.19136532716410706No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22700.18635748290970527No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCCTGTTGTTGCCTGCATATGAGGTG21430.1759313153636557No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG21310.17494616567426519No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCCTGTTGTTGC21240.1743714950221207No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20980.17223700402844128No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20900.1715802375688476No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20700.16993832141986343No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAAAAACACCCAGGCAAAGC19840.1628780819792314No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19770.16230341132708695No Hit
GTCTCTGGCGGCACCGTCAACAGTGCTAATGACTACTGGGGCTGGCTCCG19590.16082568679300116No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC19390.15918377064401698No Hit
CACCTGCACTGTCTCTGGCGGCACCGTCAACAGTGCTAATGACTACTGGG19240.15795233353227883No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19180.15745975868758358No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18770.154093830582166No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18450.1514667647437913No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17800.14613053725959269No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG17370.14260041753927669No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17190.14112269300519092No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16760.13759257328487492No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCACCTCATAT16640.13660742359548442No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC16620.13644323198058597No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16490.13537598648374627No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAAAAACA16260.13348778291241445No Hit
CTGTTATATCGTTTCGCACAGTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGGCTCACAGAGACCTG16200.1329952080677192No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15760.12938299253995397No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15280.12544239378239191No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15200.12478562732279824No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15120.12412886086320457No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15040.1234720944036109No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14860.12199436986952514No Hit
ATATAACAGTGGCTGGTACTGGTTTTTTGACCACTGGGGCCCGGGACTCC14620.1200240704907441No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14610.1199419746832949No Hit
GGTTATAACTATGTCTCCTGGTACCAAAAACACCCAGGCAAAGCCCCCAAAGTCCTCATTTATGAGGTCACTAAG14500.11903892080135359No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14500.11903892080135359No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAAC14340.11772538788216624No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13790.11321011847245974No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13780.11312802266501053No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13770.1130459268575613No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTCCCTCCGCGTCCGGGT13620.11181448974582316No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT13270.10894113648510083No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13120.10770969937336271No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13050.10713502872121825No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13020.1068887412988706No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12980.10656035806907377No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12810.10516472934243722No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12640.10376910061580065No Hit
CCTCATATGCAGGCAACAACAGGGATGTCTTCGGAACTGGGACCAAGGTC12500.10261975931151174No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12470.1023734718891641No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12440.10212718446681647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)