FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218089
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGGCTCACAGAGA55530.4558780187654597No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32920.27025939812279726No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG32750.2688637693961607No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31270.25671358989367776No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30360.24924287141579968No Hit
GTCTATTACTGTGCGAAACGATATAACAGTGGCTGGTACTGGTTTTTTGA27520.2259276621002242No Hit
GATTATTACTGCACCTCATATGCAGGCAACAACAGGGATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT27370.22469622498848604No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26810.22009885977133034No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG25790.211725087411511No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC25370.20827706349864417No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25240.2072098180018045No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25060.20573209346771867No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23500.19292514750564202No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT23160.19013389005236891No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCCTGTTGTTGCCTGCATATGAGGTGCAGTAATAAT22630.18578281225756083No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21650.1777374231275383No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC21280.17469987825191755No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCCTGTTGTTGCCTGCATATGAGGTG21180.17387892017742546No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20620.16928155496026975No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20260.16632610589209817No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19380.15910167483656779No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19290.1583628125695249No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAAAAACACCCAGGCAAAGC19220.15778814191738041No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19200.157623950302482No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18850.15475059704175967No Hit
GTCTCTGGCGGCACCGTCAACAGTGCTAATGACTACTGGGGCTGGCTCCG18720.15368335154491994No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18340.15056371086185No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG18100.14859341148306895No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17870.14670520791173713No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC17350.14243622592437827No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCCTGTTGTTGC17170.14095850139029248No Hit
CTGTTATATCGTTTCGCACAGTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGGCTCACAGAGACCTG16990.13948077685620675No Hit
CACCTGCACTGTCTCTGGCGGCACCGTCAACAGTGCTAATGACTACTGGG16920.13890610620406227No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16660.1367716152103828No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAAAAACA16520.1356222739060939No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15900.13053233384424293No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15400.12642754347178245No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15220.12494981893769667No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15160.1244572440930014No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCACCTCATAT14970.12289742375146642No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAAC14950.12273323213656802No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14910.12240484890677118No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14830.12174808244717751No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14630.12010616629819333No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14590.11977778306839648No Hit
GGTTATAACTATGTCTCCTGGTACCAAAAACACCCAGGCAAAGCCCCCAAAGTCCTCATTTATGAGGTCACTAAG14440.11854634595665835No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13910.11419526816185024No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13860.1137847891246042No Hit
GTGCTAATGACTACTGGGGCTGGCTCCGCCACTCCCCAGGTAAGGGCCTG13750.1128817352426629No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13470.11058305263408504No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13280.10902323229255007No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13090.10746341195101508No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTCCCTCCGCGTCCGGGT12900.1059035916094801No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12840.10541101676478483No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12740.10459005869029275No Hit
ATATAACAGTGGCTGGTACTGGTTTTTTGACCACTGGGGCCCGGGACTCC12680.10409748384559749No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG12640.10376910061580065No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12600.10344071738600381No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12470.1023734718891641No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12390.10171670542957043No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12360.10147041800722278No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12360.10147041800722278No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT12340.10130622639232437No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12300.10097784316252753No Hit
CAGTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGGCTCACAGA12270.10073155574017992No Hit
GGATGAGGCTGATTATTACTGCACCTCATATGCAGGCAACAACAGGGATGTCTTCGGAACTGGGACCAAGGTCAC12240.10048526831783228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC150.002221211870.0294753
GATGGAT13950.049.28133470
GGTATCA10000.044.1480141
GTGGTAT2850.039.6269381
CGACACA350.001407666439.08178721
TGGTATC2950.038.263212
GTATCAA24750.034.845511
ACGGGTT501.961604E-434.19375216
CGATGGA13800.032.99421769
TATCAAC28150.030.6204572
TATACAG902.1614323E-730.3882085
ATCAACG28800.030.0370313
TCAACGC28950.029.9958114
GGATATT4850.029.6366421
CAACGCA29450.029.4853325
AACGCAG29900.029.3857946
ACGCAGA31850.027.479327
TAAACCG500.00796475527.4349933
GTGTTAC500.00804509727.378611
TTTATAC500.0080806527.3538783
Produced by FastQC (version 0.11.5)