FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358538
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC107070.7881266479112103No Hit
GTATAATAGTTACCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT106920.7870225197970171No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55060.4052886264499042No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45400.33418277589585277No Hit
GTATCTTAGTGGTTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGT39560.2911953879832585No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38240.28147906057835703No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38020.27985967267754014No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35720.2629297082599088No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32870.24195127409023526No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32840.24173044846739658No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAGCTTCAGGGAGA32350.23812362996103162No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30110.22163531678907766No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTAC29410.21648271892284204No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29000.2134647687440469No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCTTCACTTGCCG28390.2089746477463273No Hit
GAGTGAACTCTGTCCCAGATCCACTGGCGCTGAACCTTGATGGGACCCCA27230.20043605699656541No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26800.19727088973587786No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCTTCACTTGCCGGGCCAGTCAGT26680.19638758724452315No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25760.18961560147747064No Hit
GTAGGAGACAGAGTCACCTTCACTTGCCGGGCCAGTCAGTATCTTAGTGGTTGGTTGGCCTGGTATCAGCAGAGA25480.18755456233097637No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25440.1872601281671915No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25400.18696569400340662No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25150.18512548047975103No Hit
GTGTATTACTGTGCGGGGTACCTGGAACTTGCTGCTTTTGATATCTGGGG25010.1840949609065039No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24650.18144505343243988No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24540.1806353594820314No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24280.1787215374174296No Hit
CTAAGATACTGACTGGCCCGGCAAGTGAAGGTGACTCTGTCTCCTACAGA23510.17305367976457045No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT23420.17239120289605445No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23380.17209676873226953No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23340.17180233456848465No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23080.16988851250388284No Hit
CAGTATAATAGTTACCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22940.16885799293063572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22380.16473591463764722No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21870.16098187904938988No Hit
GTGTAAGGGTAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATC21780.16031940218087384No Hit
GTCAGTATCTTAGTGGTTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGG21740.16002496801708896No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21660.1594360996895192No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21310.15685980075640138No Hit
CACCTGTACTGTCTCTGGTGGCTCCATCAGAAGTGGAGGTTACTACTGGA20120.14810038438380083No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20070.1477323416790697No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTACTTTACAAAGTGGGGTCCCAT19500.14353665484513498No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC19370.1425797438128341No Hit
GTCCCAGATCCACTGGCGCTGAACCTTGATGGGACCCCACTTTGTAAAGT19320.142211701108103No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19040.14015066196160872No Hit
GACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCG18700.13764797156943714No Hit
CCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG18380.135292498259158No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18350.13507167263631933No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18170.13374671889928733No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18080.13308424203077132No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC17910.13183289683468552No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17540.12910938081967527No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17360.12778442708264323No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16920.12454565128100943No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16810.12373595733060098No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16780.12351513170776232No Hit
GTCCTGGACCTCCTGTGCAAGAACATGGAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC16660.12263182921640764No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16660.12263182921640764No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16610.12226378651167653No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16580.12204296088883786No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCTTCACTT16370.12049718152896717No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAGCTTCAGGGA16370.12049718152896717No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15870.11681675448165603No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15830.11652232031787112No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15780.11615427761314No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG15730.11578623490840889No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT15410.11343076159812976No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15380.11320993597529108No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15280.11247385056582886No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCAACCACTAAGATACTGA15070.11092807120595817No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14970.11019198579649593No Hit
GTATTACTGTGCGGGGTACCTGGAACTTGCTGCTTTTGATATCTGGGGCC14750.10857259789567902No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14590.10739486124053946No Hit
GTATCAGCAGAGACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGT14390.105922690421615No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14270.10503938793026034No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14260.10496577938931409No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14250.10489217084836788No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14150.10415608543890564No Hit
GTCTCTGGTGGCTCCATCAGAAGTGGAGGTTACTACTGGAGCTGGATCCG14100.10378804273417452No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14060.10349360857038964No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13930.10253669753808874No Hit
GGTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCAACCACTA13870.10209504629241141No Hit
GGCCAAAAGTGTAAGGGTAACTATTATACTGTTGGCAGTAATAAGTTGCA13850.10194782921051895No Hit
TCTATAAGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGCCAGTGGATCTGGGACAGAGTTCACT13840.10187422066957273No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13790.10150617796484161No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13770.10135896088294917No Hit
ACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGCCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGC13770.10135896088294917No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC13760.10128535234200295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)