FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490386
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT91430.613465236522619No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC81260.5452278805624852No Hit
GTATTCTAGTATTCCGTTCACTTTTGGCCAGGGGACCAACCTGGAGCTCAAACGAACTGTGGCTGCACCAT72910.4892021261606054No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC66580.4467299075541504No Hit
CTAGTATTCCGTTCACTTTTGGCCAGGGGACCAACCTGGAGCTCAAACGA62700.42069638335303744No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC60890.4085518785066419No Hit
GTTTTATCCAGCTCCAACAATCAGAACTACTTAGCATGGTACCAGCAGAA59780.4011041434903441No Hit
ACTTAGGAGCCTGGCCTGCTTTCTGCTGGTACCATGCTAAGTAGTTCTGATTGTTGGAGCTGGATAAAACACT59340.39815188816856845No Hit
GAATACTAGAATACTGTTGACAGTAATAAACTGCCACATCTTCAACCTGCAGGCTGCTGATGGTGAGAGAAAAAT57140.3833906115596899No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55300.37104481657771876No Hit
CTAGAATACTGTTGACAGTAATAAACTGCCACATCTTCAACCTGCAGGCTGCTGATGGTGAGAGAAAAATCTGTC55050.36936739878125535No Hit
ACTTAGCATGGTACCAGCAGAAAGCAGGCCAGGCTCCTAAGTTGCTCATT53120.3564177333925574No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT52790.35420354190122555No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTAACCGGGATTCCGGGGTCCCT52170.3500435457659962No Hit
GTTTATTACTGTCAACAGTATTCTAGTATTCCGTTCACTTTTGGCCAGGGGACCAACCTGGAGCTCAAACGAA51860.3479635476983815No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG48010.3221313136328441No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA46900.31468357861654633No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46890.3146164819046878No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG45670.3064306830579461No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG45650.306296489634229No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTTTCTCTCAC45520.305424232380068No Hit
GTAATAAACTGCCACATCTTCAACCTGCAGGCTGCTGATGGTGAGAGAAAAATCTGTCCCAGACCCGCTGCCA45360.30435068499033135No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT43000.2885158609917162No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT42610.28589908922923324No Hit
GTACCAGCAGAAAGCAGGCCAGGCTCCTAAGTTGCTCATTTACTGGGCATCTAACCGGGATTCCGGGGTCCCTGA41980.2816719963821453No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG41280.2769752265520476No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40210.269795878383184No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC40160.2694603948238913No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATC39510.26509910855308627No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC38800.26033524201113No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38150.255973955740325No Hit
GGATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA38020.255101698486164No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGGTTGGTCCCCTGGCCAAAAGTG35930.2410784857077294No Hit
GTCAGGGACCCCGGAATCCCGGTTAGATGCCCAGTAAATGAGCAACTTAGGAGCCTGGCCTGCTTTCTGCTGGTA35170.23597913560648048No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATCCAGCTCCAA33970.2279275301834558No Hit
CTTTTGGCCAGGGGACCAACCTGGAGCTCAAACGAACTGTGGCTGCACCA32830.22027850503158242No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGC32740.21967463462485556No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32490.21799721682839213No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31650.21236109303227488No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG31520.21148883577811387No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT30490.20457787445668438No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30240.2029004566602209No Hit
GAACTACTTAGCATGGTACCAGCAGAAAGCAGGCCAGGCTCCTAAGTTGC29790.19988110462658668No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29530.19813659011826468No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTTTCTCTCACCATCAGCAGCC29370.19706304272852807No Hit
CTGCTGGTACCATGCTAAGTAGTTCTGATTGTTGGAGCTGGATAAAACAC28560.19162820906798642No Hit
GGCCAAAAGTGAACGGAATACTAGAATACTGTTGACAGTAATAAACTGCC27980.1877365997801912No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGGTTGGTCC27950.18753530964461554No Hit
ATGCTAAGTAGTTCTGATTGTTGGAGCTGGATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGC27050.1814966055773471No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26360.17686693245910792No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATCCAGCTCCAACAATCAGAACTA25980.17431725740848344No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT25880.17364629028989803No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25700.17243854947644435No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC25610.1718346790697175No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT25470.17089532510369795No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT25160.16881532703608326No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24900.16707081252776126No Hit
GGTTGAAGATGTGGCAGTTTATTACTGTCAACAGTATTCTAGTATTCCGTTCACTTTTGGCCAGGGGACCAAC24200.16237404269766356No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG23620.15848243340986834No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23540.15794565971500002No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG23520.15781146629128293No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23510.1577443695794244No Hit
GCAGAAAGCAGGCCAGGCTCCTAAGTTGCTCATTTACTGGGCATCTAACCGGGATTCCGGGGTCCCTGACCGATT23440.1572746925964146No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA23420.15714049917269754No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT22640.15190695564773152No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22630.15183985893587298No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22630.15183985893587298No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22600.15163856880029736No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22490.15090050496985344No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC22480.1508334082579949No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22250.1492901838852485No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21910.1470088956820582No Hit
GTAAATGAGCAACTTAGGAGCCTGGCCTGCTTTCTGCTGGTACCATGCTA21890.14687470225834112No Hit
GGATCTCTGGTGCCTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG21860.14667341212276552No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21700.1455998647330289No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21580.14479470419072643No Hit
GATTTTTCTCTCACCATCAGCAGCCTGCAGGTTGAAGATGTGGCAGTTTATTACTGTCAACAGTATTCTAGTATT21540.14452631734329227No Hit
GCATGGTACCAGCAGAAAGCAGGCCAGGCTCCTAAGTTGCTCATTTACTGGGCATCTAACCGGGATTCCGGGGTC21530.14445922063143374No Hit
GGTCCAGCCAGAGTGTTTTATCCAGCTCCAACAATCAGAACTACTTAGCATGGTACCAGCAGAAAGCAGGCCAGG21320.14305018968240443No Hit
GCTTTCTGCTGGTACCATGCTAAGTAGTTCTGATTGTTGGAGCTGGATAA20530.13774954944557988No Hit
ACCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20460.1372798724625701No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT20370.13667600205584324No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20250.13587084151354079No Hit
GGACCAACCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC20150.1351998743949554No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20120.13499858425937977No Hit
TCTTCAACCTGCAGGCTGCTGATGGTGAGAGAAAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC19870.13332116646291634No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19860.13325406975105777No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19750.13251600592061386No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19660.131912135513887No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT19640.13177794209016994No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC19370.1299663308699894No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19140.128423106497243No Hit
CTGCAGGTCCAGCCAGAGTGTTTTATCCAGCTCCAACAATCAGAACTACTTAGCATGGTACCAGCAGAAAGCAGG19110.12822181636166738No Hit
GGTTGGTCCCCTGGCCAAAAGTGAACGGAATACTAGAATACTGTTGACAGTAATAAACTGCCACATCTTCAAC18970.12728246239564783No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA18820.12627601171776975No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18600.12479988405688192No Hit
CTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG18580.12466569063316482No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18290.12271988598926721No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17870.11990182409120859No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17600.11809021287102804No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17480.11728505232872558No Hit
TCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCT17360.11647989178642311No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCCGGGGACATCGTGATGA17290.11601021480341335No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17290.11601021480341335No Hit
GTCTGGGACAGATTTTTCTCTCACCATCAGCAGCCTGCAGGTTGAAGATG17270.11587602137969627No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG17240.11567473124412064No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17200.11540634439668648No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTTTCTCTCACCATCAG17090.11466828056624256No Hit
CCACTGAATCGGTCAGGGACCCCGGAATCCCGGTTAGATGCCCAGTAAAT17030.11426570029509134No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16770.11252118578676934No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16690.11198441209190103No Hit
CATTTACTGGGCATCTAACCGGGATTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTTT16250.10903215677012532No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16150.10836118965153994No Hit
GTGTTTTATCCAGCTCCAACAATCAGAACTACTTAGCATGGTACCAGCAGAAAGCAGGCCAGGCTCCTAAGTTG15940.10695215870251062No Hit
CAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT15790.10594570802463255No Hit
CCATCAGCAGCCTGCAGGTTGAAGATGTGGCAGTTTATTACTGTCAACAG15670.10514054748233008No Hit
AAGTAGTTCTGATTGTTGGAGCTGGATAAAACACTCTGGCTGGACCTGCA15640.10493925734675447No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG15610.10473796721117885No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT15440.10359732310958368No Hit
GCTCATTTACTGGGCATCTAACCGGGATTCCGGGGTCCCTGACCGATTCA15350.10299345270285686No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15340.10292635599099831No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC15230.10218829216055439No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG15210.1020540987368373No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT14970.10044377765223238No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGAGA14920.10010829409293968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG150.00250986467.9055118
GTGGTAT3000.052.0556531
GGTATCA13900.048.359491
CTAGCGC1850.046.00714528
TCGTATG1400.044.3288440
ACTAGCG1950.043.61258727
TACTGAA5900.043.0382770
TGGTATC3650.042.785472
CGTATGC1550.042.33402341
GACTAGC2050.041.47120326
TAGCGCT2100.040.55466529
ATGCCGT1550.040.28880344
TCTCCGA2250.039.23034311
CGCTCAT1700.038.17778832
ACGAGAC2150.037.91983822
CACGAGA2150.037.9134721
GAGACTA2250.037.7594924
TCCGAGC2350.037.56096613
AGACTAG2300.036.94856325
CGAGACT2300.036.9287123
Produced by FastQC (version 0.11.5)