FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490386
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT86830.5826007490676912No Hit
GTATTCTAGTATTCCGTTCACTTTTGGCCAGGGGACCAACCTGGAGCTCAAACGAACTGTGGCTGCACCAT68080.4567944143329312No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65170.4372692711820964No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC63120.423514445251096No Hit
GAATACTAGAATACTGTTGACAGTAATAAACTGCCACATCTTCAACCTGCAGGCTGCTGATGGTGAGAGAAAAAT60520.40606930016787596No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58970.3956693098298025No Hit
CTAGAATACTGTTGACAGTAATAAACTGCCACATCTTCAACCTGCAGGCTGCTGATGGTGAGAGAAAAATCTGTC57100.38312222471225577No Hit
GTAATAAACTGCCACATCTTCAACCTGCAGGCTGCTGATGGTGAGAGAAAAATCTGTCCCAGACCCGCTGCCA54940.3686293349508114No Hit
CTAGTATTCCGTTCACTTTTGGCCAGGGGACCAACCTGGAGCTCAAACGA54540.36594546647646986No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT52330.3511170931557328No Hit
GTTTTATCCAGCTCCAACAATCAGAACTACTTAGCATGGTACCAGCAGAA51450.3452125825121814No Hit
ACTTAGGAGCCTGGCCTGCTTTCTGCTGGTACCATGCTAAGTAGTTCTGATTGTTGGAGCTGGATAAAACACT51190.3434680680038594No Hit
GTTTATTACTGTCAACAGTATTCTAGTATTCCGTTCACTTTTGGCCAGGGGACCAACCTGGAGCTCAAACGAA49310.33085388617445416No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48410.3248151821071857No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45520.305424232380068No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTAACCGGGATTCCGGGGTCCCT45390.304551975125907No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44110.29596359600801403No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42280.28368489773790145No Hit
ACTTAGCATGGTACCAGCAGAAAGCAGGCCAGGCTCCTAAGTTGCTCATT41580.27898812790780375No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC41100.2757674857385939No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41060.27549909889115975No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT40680.2729494238405353No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTTTCTCTCAC38470.2581210505197982No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38450.25798685709608116No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC38280.256846212994486No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37390.25087460563907604No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36770.24671460950384666No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35430.23772365011480245No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGGTTGGTCCCCTGGCCAAAAGTG35120.2356436520471878No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA34500.23148365591195838No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34100.2287997874376168No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT33660.22584753211584113No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATC33360.22383463076008497No Hit
GTACCAGCAGAAAGCAGGCCAGGCTCCTAAGTTGCTCATTTACTGGGCATCTAACCGGGATTCCGGGGTCCCTGA32320.21685657272679698No Hit
GTCAGGGACCCCGGAATCCCGGTTAGATGCCCAGTAAATGAGCAACTTAGGAGCCTGGCCTGCTTTCTGCTGGTA31820.21350173713387002No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29370.19706304272852807No Hit
CTTTTGGCCAGGGGACCAACCTGGAGCTCAAACGAACTGTGGCTGCACCA29270.19639207560994265No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29040.19484885123719628No Hit
GGCCAAAAGTGAACGGAATACTAGAATACTGTTGACAGTAATAAACTGCC28770.19303724001701572No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATCCAGCTCCAA28450.1908901452375425No Hit
GGATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA27800.18652885896673746No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTTTCTCTCACCATCAGCAGCC27410.18391208720425445No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGAATCCCGGTT27100.18183208913663979No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGC26950.18082563845876168No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA26880.18035596147575192No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG26870.18028886476389339No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26700.1791482206622982No Hit
TCTTCAACCTGCAGGCTGCTGATGGTGAGAGAAAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC26240.17606177191680544No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25930.17398177384919075No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25910.17384758042547369No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25290.16968758429024428No Hit
GAACTACTTAGCATGGTACCAGCAGAAAGCAGGCCAGGCTCCTAAGTTGC24970.16754048951077102No Hit
CTGCTGGTACCATGCTAAGTAGTTCTGATTGTTGGAGCTGGATAAAACAC24360.16344759008740017No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24260.1627766229688148No Hit
GGTTGAAGATGTGGCAGTTTATTACTGTCAACAGTATTCTAGTATTCCGTTCACTTTTGGCCAGGGGACCAAC24210.1624411394095221No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT24090.16163597886721962No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC24060.16143468873164402No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT23740.15928759395217076No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23600.15834823998615125No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22920.1537856635797706No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22820.15311469646118522No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22740.15257792276631693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22340.14989405429197536No Hit
ATGCTAAGTAGTTCTGATTGTTGGAGCTGGATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGC22240.14922308717339No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGGTTGGTCC22220.1490888937496729No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22170.14875341019038021No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21630.14513018775001912No Hit
GATTTTTCTCTCACCATCAGCAGCCTGCAGGTTGAAGATGTGGCAGTTTATTACTGTCAACAGTATTCTAGTATT21430.14378825351284835No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21240.1425134159875361No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21240.1425134159875361No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21200.14224502914010195No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATCCAGCTCCAACAATCAGAACTA20910.14029922449620433No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20820.1396953540894775No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20740.13915858039460918No Hit
GGTTGGTCCCCTGGCCAAAAGTGAACGGAATACTAGAATACTGTTGACAGTAATAAACTGCCACATCTTCAAC20450.13721277575071156No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT19790.13278439276804802No Hit
GGATCTCTGGTGCCTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG19740.13244890920875532No Hit
GTAAATGAGCAACTTAGGAGCCTGGCCTGCTTTCTGCTGGTACCATGCTA19570.13130826510716015No Hit
ACCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG19350.1298321374462723No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19230.12902697690396983No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19210.12889278348025276No Hit
GGACCAACCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19070.12795342951423322No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18970.12728246239564783No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18670.12526956103989167No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA18610.12486698076874043No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18500.12412891693829652No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18440.12372633666714529No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18150.12178053202324768No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC18070.12124375832837937No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18020.12090827476908667No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG17750.11909666354890612No Hit
GCAGAAAGCAGGCCAGGCTCCTAAGTTGCTCATTTACTGGGCATCTAACCGGGATTCCGGGGTCCCTGACCGATT17520.11755343917615974No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCCGGGGACATCGTGATGA17470.11721795561686703No Hit
CTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG17380.1166140852101402No Hit
GCTTTCTGCTGGTACCATGCTAAGTAGTTCTGATTGTTGGAGCTGGATAA17310.11614440822713042No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17300.11607731151527188No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17300.11607731151527188No Hit
GGTCCAGCCAGAGTGTTTTATCCAGCTCCAACAATCAGAACTACTTAGCATGGTACCAGCAGAAAGCAGGCCAGG17180.11527215097296942No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17150.1150708608373938No Hit
GCATGGTACCAGCAGAAAGCAGGCCAGGCTCCTAAGTTGCTCATTTACTGGGCATCTAACCGGGATTCCGGGGTC17140.11500376412553527No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16980.11393021673579865No Hit
GTCTGGGACAGATTTTTCTCTCACCATCAGCAGCCTGCAGGTTGAAGATG16900.11339344304093033No Hit
GAGAAAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG16840.11299086276977911No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16490.11064247785473025No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16390.10997151073614488No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG16040.10762312582109602No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16000.10735473897366186No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15500.10399990338073493No Hit
CTGCAGGTCCAGCCAGAGTGTTTTATCCAGCTCCAACAATCAGAACTACTTAGCATGGTACCAGCAGAAAGCAGG15440.10359732310958368No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT15420.10346312968586661No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15240.10225538887241292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3050.049.9955182
GTGGTAT3150.049.5140231
GGTATCA11850.045.4945871
CATCGCG407.0263847E-445.00887369
ATCGCGG700.001102483141.0707570
GTATCAA33800.040.2261621
CGCGAAT400.002129946635.9215662
TATCAAC38250.035.2590072
TACTGAA5650.034.3467870
CGCGCTT400.00284495933.86311312
ATCAACG40000.033.6226773
TCAACGC40200.033.4475444
CAACGCA40750.032.9107785
AACGCAG41400.032.3122636
CAAACGC552.9908915E-431.8235149
ACGCAGA43350.030.9368937
CGCAGAG43700.030.844118
TGTTAGA25800.030.64387370
TTTCGAT950.004931175630.26265770
AGAGTAC44700.030.22680911
Produced by FastQC (version 0.11.5)