FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358197
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAATGACTGGTCGGGCGCTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT76030.5597862460305832No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64820.47725035469817706No Hit
GTCATTACACTGTTGACAGTAATAAACTGCAAAATCTTCAGATTGCAGGTAGTTGATGGTGAGGGTGAACT64010.4712865659399925No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56510.4160662996605058No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC50150.3692395138555011No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42680.3142401286411323No Hit
GAGTTCACCCTCACCATCAACTACCTGCAATCTGAAGATTTTGCAGTTTA39310.2894278223262163No Hit
GTCTGGGACAGAGTTCACCCTCACCATCAACTACCTGCAATCTGAAGATT38930.28662999550138896No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38040.28007719056955654No Hit
CTGGTATCCCACCCAGGTTCAGTGGCACTGGGTCTGGGACAGAGTTCACC36580.2693276454004831No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35380.26049240279576524No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34730.25570664638487645No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAGCAGAAACCGGGCCAGGCTCCCAGACT33390.2458406254762748No Hit
GTCCCAGACCCAGTGCCACTGAACCTGGGTGGGATACCAGTGGCCCGAGT32730.24098124204368No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31940.23516470732890737No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31520.23207237241725612No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31270.23023169687460657No Hit
GTGTTAGCACCAACTTAGCCTGGTACCAGCAGAAACCGGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCTA31010.228317394310251No Hit
CTCCCAGACTCCTCATCTATGATGCATCTACTCGGGCCACTGGTATCCCA30340.2233843838559502No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC30160.22205909746524252No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29480.21705245998923572No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29070.21403375209929046No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28380.2089534876015777No Hit
GTTTATTACTGTCAACAGTGTAATGACTGGTCGGGCGCTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA27850.20505125545116062No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27020.19894021264956407No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26380.19422808326038124No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC26170.1926819158045556No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC26080.19201927260920176No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA25800.18995771600143424No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTA25530.18796978641537274No Hit
GGCCACTGGTATCCCACCCAGGTTCAGTGGCACTGGGTCTGGGACAGAGT25460.18745439726343086No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25440.18730714322001887No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGCG24940.18362579213471977No Hit
GTAATATGTGGCTGTGTCCACAGGTTCCATGTAGGTCATTGTGAAGACCA24560.18082796530989245No Hit
GGATACCAGTGGCCCGAGTAGATGCATCATAGATGAGGAGTCTGGGAGCCTGGCCCGGTTTCTGCTGGTACCAGG24220.17832464657188907No Hit
GGCCAAAAGCGCCCGACCAGTCATTACACTGTTGACAGTAATAAACTGCA24170.17795651146335914No Hit
GTAATAAACTGCAAAATCTTCAGATTGCAGGTAGTTGATGGTGAGGGTGAACTCTGTCCCAGACCCAGTGCCA24140.1777356303982412No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23590.17368614420441217No Hit
CTACTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTG23410.1723608578137045No Hit
GTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAGCAGAAACCGGGCCAGGCTCCCAGACTCCTCATCTATGATG23050.16971028503228913No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22160.16315748010045672No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21420.15770908049421403No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT21300.15682555623374222No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21220.15623654006009438No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21120.15550026984303456No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20650.15203979982285337No Hit
AACTTAGCCTGGTACCAGCAGAAACCGGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCTACTCGGGCCA20280.14931560001973204No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20210.14880021086779016No Hit
CTGCTACTCTGGCTCCCAGATAGCAATGGAGAAATAGTGATGACGCAGTC20030.1474749244770825No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG20020.1474012974553765No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19830.14600238404296284No Hit
GTCTGGGAGCCTGGCCCGGTTTCTGCTGGTACCAGGCTAAGTTGGTGCTA19270.14187927082742782No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19010.1399649682630723No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18950.1395232061328364No Hit
GGGTGAACTCTGTCCCAGACCCAGTGCCACTGAACCTGGGTGGGATACCA18810.13849242782895266No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18550.13657812526459712No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18480.13606273611265524No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18020.13267589311418004No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17140.12619671520405362No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16930.12465054774822798No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16700.1229571262489904No Hit
ACTGTCAACAGTGTAATGACTGGTCGGGCGCTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTG16610.12229448305363655No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16260.11971753729392717No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16250.1196439102722212No Hit
CCAGTGGCCCGAGTAGATGCATCATAGATGAGGAGTCTGGGAGCCTGGCC16190.11920214814198529No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCAATGGAGAAATAGTGATGACGCAGTCTC16130.11876038601174939No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16060.11824499685980751No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15300.11264934321015287No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGC15210.11198670001479902No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15010.1105141595806794No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14960.11014602447214947No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14950.11007239745044349No Hit
CAGTAATATGTGGCTGTGTCCACAGGTTCCATGTAGGTCATTGTGAAGAC14840.10926250021167769No Hit
CCCTCACACTGACCTGCTCCTTCTCTGGGTTCTCACTCACCACTACTGGAGTGGGTGTGGGCTGGATCCGTCAGC14840.10926250021167769No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14740.10852622999461786No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAGCAGAAACCGGGCCAGG14720.10837897595120591No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14690.10815809488608796No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14650.10786358679926403No Hit
GTTGACAGTAATAAACTGCAAAATCTTCAGATTGCAGGTAGTTGATGGTG14540.10705368956049822No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14400.10602291125661448No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT14230.10477125188761277No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCAATGGAGAAATAGTGATGACGCAGTCTCCAGCCACC14160.10425586273567089No Hit
CATCTACTCGGGCCACTGGTATCCCACCCAGGTTCAGTGGCACTGGGTCT14080.10366684656202305No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14030.10329871145349312No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13990.1030042033666692No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13900.10234156017131535No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13870.10212067910619742No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13710.10094264675890169No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13600.10013274952013589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT400.00937331948.67524370
GTGGTAT3150.047.7293661
AATGGTA4150.043.78816670
GGTATCA12100.043.2065541
CGTATGC1500.039.14102641
ATCTCGT1450.037.93241537
TGCCGTC1650.037.8392445
ACGAGAC1550.037.46975322
GCCGTCT1600.036.8883246
TCGTATG1600.036.6565140
ACATGCG1600.036.34030527
GTATCAA28150.035.565821
ATGCGCA1700.034.2253529
GTCTAAC400.002721834234.169891
GCTTGAC400.002721834234.169891
GTATGCC1950.033.6857742
ATGCCGT1850.031.83669744
TGTTAGA13750.031.62415770
CACGAGA1850.031.39357621
CATGCGC1900.030.60800228
Produced by FastQC (version 0.11.5)