FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358197
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAATGACTGGTCGGGCGCTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT74450.548153176601038No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64290.47334812254776004No Hit
GTCATTACACTGTTGACAGTAATAAACTGCAAAATCTTCAGATTGCAGGTAGTTGATGGTGAGGGTGAACT60020.44190938427930554No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47290.3481821856475901No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC44560.328082008721857No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43370.3193203931388451No Hit
GTCTGGGACAGAGTTCACCCTCACCATCAACTACCTGCAATCTGAAGATT39410.29016409254327613No Hit
GAGTTCACCCTCACCATCAACTACCTGCAATCTGAAGATTTTGCAGTTTA39220.28876517913086247No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36270.2670452077275977No Hit
CTGGTATCCCACCCAGGTTCAGTGGCACTGGGTCTGGGACAGAGTTCACC36190.26645619155394984No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34160.2515099061476354No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34150.2514362791259295No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33110.2437790688685073No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32110.23641636669790908No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAGCAGAAACCGGGCCAGGCTCCCAGACT32090.23626911265449713No Hit
GTCCCAGACCCAGTGCCACTGAACCTGGGTGGGATACCAGTGGCCCGAGT31290.23037895091801852No Hit
GTGTTAGCACCAACTTAGCCTGGTACCAGCAGAAACCGGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCTA30230.22257448661718437No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC29120.21440188720782036No Hit
CTCCCAGACTCCTCATCTATGATGCATCTACTCGGGCCACTGGTATCCCA28440.2093952497318136No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27250.20063363414880167No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27180.20011824499685982No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26910.19813031541079826No Hit
GGCCACTGGTATCCCACCCAGGTTCAGTGGCACTGGGTCTGGGACAGAGT26880.1979094343456803No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26120.1923137806960257No Hit
GTTTATTACTGTCAACAGTGTAATGACTGGTCGGGCGCTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA25990.19135662941384793No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC25860.19039947813167013No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTA25590.18841154854560863No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25320.1864236189595471No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24970.18384667319983772No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24580.1809752193533044No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24480.18023894913624458No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGCG24390.17957630594089077No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC24270.17869278168041897No Hit
GGGTGAACTCTGTCCCAGACCCAGTGCCACTGAACCTGGGTGGGATACCA23980.17655759805094548No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23650.17412790633464806No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA23540.17331800909588227No Hit
GTAATAAACTGCAAAATCTTCAGATTGCAGGTAGTTGATGGTGAGGGTGAACTCTGTCCCAGACCCAGTGCCA23530.17324438207417628No Hit
GTAATATGTGGCTGTGTCCACAGGTTCCATGTAGGTCATTGTGAAGACCA22770.16764872842452164No Hit
GGCCAAAAGCGCCCGACCAGTCATTACACTGTTGACAGTAATAAACTGCA22510.1657344258601661No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22260.16389375031751655No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22070.16249483690510286No Hit
GGATACCAGTGGCCCGAGTAGATGCATCATAGATGAGGAGTCTGGGAGCCTGGCCCGGTTTCTGCTGGTACCAGG21780.16035965327562937No Hit
GTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAGCAGAAACCGGGCCAGGCTCCCAGACTCCTCATCTATGATG21500.15829809666786188No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21490.1582244696461559No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20960.15432223749573884No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT20540.15122990258408758No Hit
CTGCTACTCTGGCTCCCAGATAGCAATGGAGAAATAGTGATGACGCAGTC20200.14872658384608417No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20030.1474749244770825No Hit
CTACTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTG19890.14644414617319873No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19580.1441617085003133No Hit
AACTTAGCCTGGTACCAGCAGAAACCGGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCTACTCGGGCCA19520.14371994637007737No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19330.14232103295766374No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19110.14070123848013213No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG18930.13937595208942444No Hit
GTCTGGGAGCCTGGCCCGGTTTCTGCTGGTACCAGGCTAAGTTGGTGCTA18790.1383451737855407No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18540.13650449824289113No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18540.13650449824289113No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18030.13274952013588603No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18020.13267589311418004No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17770.1308352175715305No Hit
CCCTCACACTGACCTGCTCCTTCTCTGGGTTCTCACTCACCACTACTGGAGTGGGTGTGGGCTGGATCCGTCAGC17560.12928905011570488No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17290.12730112052964335No Hit
ACTGTCAACAGTGTAATGACTGGTCGGGCGCTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTG16240.11957028325051522No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16000.11780323472957163No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15560.11456364577450841No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15500.11412188364427253No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCAATGGAGAAATAGTGATGACGCAGTCTC15080.11102954873262126No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15000.11044053255897342No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14990.11036690553726741No Hit
CCAGTGGCCCGAGTAGATGCATCATAGATGAGGAGTCTGGGAGCCTGGCC14970.11021965149385546No Hit
GTTGACAGTAATAAACTGCAAAATCTTCAGATTGCAGGTAGTTGATGGTG14890.10963063532020759No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14840.10926250021167769No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14830.10918887318997171No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14610.1075690787124401No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14590.10742182466902814No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14430.10624379232173241No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14360.10572840316979053No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14360.10572840316979053No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAGCAGAAACCGGGCCAGG14350.10565477614808455No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCAATGGAGAAATAGTGATGACGCAGTCTCCAGCCACC14320.1054338950829666No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGC14290.10521301401784865No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14180.10440311677908284No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14060.10351959251861106No Hit
CAGTAATATGTGGCTGTGTCCACAGGTTCCATGTAGGTCATTGTGAAGAC13970.10285694932325722No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13920.10248881421472732No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13650.10050088462866578No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13600.10013274952013589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)