FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322670
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATAGTTACCCATACACTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT81610.6170095337461348No Hit
CTATTAGACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC71930.5438242343139256No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44570.33696991691049166No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43230.3268388940552065No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG39670.2999236393053445No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33780.25539250153099413No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27760.20987850333038477No Hit
CTCCTGATCTATGCGGCGTCTACCTTAGAAACTGGGGTCCCATCAAGGTT27490.207837177829693No Hit
GTAGGGGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTTTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAA27250.20602266627352248No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC27170.20541782908813233No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27150.2052666197917848No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27020.20428375936552579No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26780.20246924780935532No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26410.19967187582692583No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26190.1980085735671029No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26010.19664768989997505No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTCTAATAGTTAC24590.18591182985929974No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24430.18470215548851943No Hit
CCTTCCACCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCG24230.18319006252504405No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23860.18039269054261456No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23840.18024148124626702No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23770.17971224870905064No Hit
GTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA23490.17759531856018507No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23370.17668806278209986No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23200.17540278376314575No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23150.17502476052227692No Hit
CTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCA21960.16602780738959833No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21810.16489373766699178No Hit
CCCCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGCCGGATCCAGCCCCAGTAGTAACCACTGCTGATAGAGTAA21520.16270120286995246No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21190.16020624948021803No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC20630.15597238918248693No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20250.15309941255188367No Hit
GTCTCTGGTTACTCTATCAGCAGTGGTTACTACTGGGGCTGGATCCGGCA19800.14969720338406403No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19740.14924357549502143No Hit
GTAATAAATGGCCGTGTCTGCGGCGGTCACATATTTCAACTCCAGGGAAA19370.14644620351259194No Hit
GGATTGGGGGTGTCCATTTTAGTGGGAACACCTACTCCAATCCGTCCCTC19050.14402685477103133No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18890.14281718040025101No Hit
GAGTTTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCGGCGT18880.14274157575207724No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18120.13699562249087074No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC18030.1363151806573068No Hit
GGTTACTACTGGGGCTGGATCCGGCAGCCCCCCGGGAAGGGGCTGGAGTG17790.13450066910113634No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17500.13230813430409702No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17310.1308716459887954No Hit
GGCCAAAAGTGTATGGGTAACTATTAGACTGTTGGCAGTAATAAGTTGCA17260.13049362274792656No Hit
GTGTATGGGTAACTATTAGACTGTTGGCAGTAATAAGTTGCAAAATCATC17220.13019120415523147No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT17000.12852790189540853No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16740.12656218104289052No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16700.12625976245019543No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT16590.12542811132028397No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16270.12300876257872334No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16250.1228575532823758No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16120.1218746928561168No Hit
CCCATACACTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGG16000.12096743707803155No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT15880.12006018129994632No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15830.11968215805907748No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15770.11922853017003485No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG15350.11605313494673652No Hit
CACCTACTCCAATCCGTCCCTCAAGAGTCGAGTCACCATTTCACTAGACA15220.11507027452047752No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15030.1136337862051759No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14960.1131045536679595No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTT14770.11166806535265789No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT14600.11038278633370378No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCCTGGCCAAAAGTG14560.11008036774100873No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14550.11000476309283495No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC14520.10977794914831364No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC14490.10955113520379232No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT14420.10902190266657594No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT14240.10766101899944809No Hit
CCTTGGTCCCCTGGCCAAAAGTGTATGGGTAACTATTAGACTGTTGGCAGTAATAAGTTGCAAAATCATCAGG14030.10607332138779892No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13850.10471243772067107No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT13840.1046368330724973No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13700.10357836799806451No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13520.10221748433093666No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTACTA13460.10176385644189406No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13370.10108341460833012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)