FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322670
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATAGTTACCCATACACTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT80200.6063492783536332No Hit
CTATTAGACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC71900.5435974203694043No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44480.33628947507692775No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG41790.31595182471818367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38080.2879025002457151No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35540.2686989196095776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33670.2545608504010827No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA29080.21985831688932236No Hit
CTCCTGATCTATGCGGCGTCTACCTTAGAAACTGGGGTCCCATCAAGGTT27270.20617387556987002No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26690.2017888059757914No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26240.19838659680797174No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26020.1967232945481488No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24850.18787755071181775No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC24720.18689469028555877No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24480.18508017872938828No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24300.18371929506226042No Hit
GTAGGGGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTTTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAA23810.18001466730174573No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC23770.17971224870905064No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23200.17540278376314575No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTCTAATAGTTAC22890.1730590396697589No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22220.16799352824211633No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22130.16731308640855239No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22020.16648143527864093No Hit
CTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCA21950.16595220274142455No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21650.16368406329621144No Hit
GTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA21580.16315483075899506No Hit
CCTTCCACCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCG21400.1617939470918672No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20540.15529194734892302No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20340.1537798543854476No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19960.15090687775484438No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19450.1470510406979821No Hit
CCCCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGCCGGATCCAGCCCCAGTAGTAACCACTGCTGATAGAGTAA19110.14448048266007396No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC19030.1438756454746838No Hit
GTAATAAATGGCCGTGTCTGCGGCGGTCACATATTTCAACTCCAGGGAAA19020.14380004082651002No Hit
GTCTCTGGTTACTCTATCAGCAGTGGTTACTACTGGGGCTGGATCCGGCA18950.14327080828929362No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC18360.1388101340470412No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18310.13843211080617235No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18200.13760045967626092No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18160.13729804108356583No Hit
GAGTTTTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCGGCGT18120.13699562249087074No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT17450.13193011106322816No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17370.13132527387783802No Hit
GTGTATGGGTAACTATTAGACTGTTGGCAGTAATAAGTTGCAAAATCATC17330.13102285528514293No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17320.13094725063696916No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA17150.12966197161801507No Hit
GGTTACTACTGGGGCTGGATCCGGCAGCCCCCCGGGAAGGGGCTGGAGTG16960.12822548330271344No Hit
GGCCAAAAGTGTATGGGTAACTATTAGACTGTTGGCAGTAATAAGTTGCA16950.12814987865453967No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16900.12777185541367084No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16860.12746943682097575No Hit
GGATTGGGGGTGTCCATTTTAGTGGGAACACCTACTCCAATCCGTCCCTC16700.12625976245019543No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC16620.1256549252648053No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16020.1211186463743791No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC16010.12104304172620532No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC15910.12028699524446763No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15770.11922853017003485No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT15440.11673357678030045No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14880.11249971648256935No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14850.11227290253804803No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT14660.11083641422274641No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT14530.10985355379648741No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC14420.10902190266657594No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14400.1088706933702284No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCCTGGCCAAAAGTG14370.10864387942570708No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT14110.10667815857318909No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14100.10660255392501532No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14080.10645134462866777No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTACTA13930.10531727490606121No Hit
CCCATACACTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGG13820.10448562377614976No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC13810.10441001912797598No Hit
CCTTGGTCCCCTGGCCAAAAGTGTATGGGTAACTATTAGACTGTTGGCAGTAATAAGTTGCAAAATCATCAGG13740.1038807865907596No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13520.10221748433093666No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTT13370.10108341460833012No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13350.10093220531198258No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13330.10078099601563503No Hit
ACTTATTACTGCCAACAGTCTAATAGTTACCCATACACTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAA13230.10002494953389736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC150.002481986568.096651
CCTAACG209.109231E-568.065752
GCTTAGT200.007752857651.0724871
CTAACGC306.7566126E-445.368593
GTGGTAT1900.043.008411
ACCTAAC451.082461E-437.8314741
TAACGGC853.1250238E-936.3398536
TTAACGG853.1523086E-936.30662535
GTGAGCG7850.036.25148870
GTATCAA26700.035.1960221
TAGACCT400.002771839534.04317519
TAGTGAA9700.032.8301170
AAAACCG1701.4161742E-731.88506170
GCGGTAC1101.0040822E-931.5218144
TATCAAC30150.031.2673532
CGTGCAA802.2542117E-630.98430457
GATAGTA553.5283138E-430.9389782
TCAACGC31350.030.498984
ATCAACG31250.030.4876943
TGTTAGA13350.030.45202470
Produced by FastQC (version 0.11.5)