FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815952
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTATTGGGCATCTGCCCGGAGATCCGGGGTCCCT57170.7006539600368649No Hit
GTTTAGGAGGCTGTCCCGGATGTTGCTGGTACCAAGCTAAGTAGTTCTTGTTTATGGACTTGTCCAAAAGACT47240.5789556248406769No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42460.5203737474753417No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37320.4573798458732866No Hit
GTTCAGTGCCAGCGGGTCTGGGACGGATTTCACTCTCACCATCAGCCGCC31080.38090475910347665No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27850.34131909720179615No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26850.32906347432201893No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26800.3284506931780301No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23790.2915612683099006No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22640.2774673019981568No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21550.26410867305919955No Hit
GTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGTG19900.24388689530756713No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19710.24155832696040944No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19340.23702374649489186No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19310.23665607780849857No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGGA19190.23518540306292526No Hit
CTTTTGGACAAGTCCATAAACAAGAACTACTTAGCTTGGTACCAGCAACA19150.2346951781477342No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19130.23445006569013863No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCC18730.22954781653822776No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18540.22721924819107006No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18540.22721924819107006No Hit
ACCTTGGTCCCTCCGCCGAAAGTGGGAGGAGGACGAAAGTACTGCTGACAGTAATAAGTTGCCACATCTTCAGC18210.22317489264074356No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18040.22109143675118145No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17820.21839519971763047No Hit
GTCCCTGACCGGTTCAGTGCCAGCGGGTCTGGGACGGATTTCACTCTCAC17420.21349295056571957No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17300.21202227582014627No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17110.2096937074729886No Hit
GCACTGAACCGGTCAGGGACCCCGGATCTCCGGGCAGATGCCCAATAAAT16400.20099221522834673No Hit
GACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT16220.19878620310998688No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA15940.19535462870364922No Hit
GATTTCACTCTCACCATCAGCCGCCTGCAGGCTGAAGATGTGGCAACTTATTACTGTCAGCAGTACTTTCGTCCT15840.19412906641567151No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14940.18309900582387198No Hit
GTGCCAGCGGGTCTGGGACGGATTTCACTCTCACCATCAGCCGCCTGCAGGCTGAAGATGTGGCAACTTATTA14830.1817508873070965No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT14750.1807704374767143No Hit
GTAATAAGTTGCCACATCTTCAGCCTGCAGGCGGCTGATGGTGAGAGTGAAATCCGTCCCAGACCCGCTGGCA14280.17501029472321902No Hit
CAGTAATAAGTTGCCACATCTTCAGCCTGCAGGCGGCTGATGGTGAGAGTGAAATCCGTCCCAGACCCGCTGGCA14090.17268172637606136No Hit
ACTTATTACTGTCAGCAGTACTTTCGTCCTCCTCCCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAA14010.17170127654567915No Hit
ATCCGGGACAGCCTCCTAAACTGCTCATTTATTGGGCATCTGCCCGGAGATCCGGGGTCCCTGACCGGTTCAGTG13990.17145616408808362No Hit
GTCCATAAACAAGAACTACTTAGCTTGGTACCAGCAACATCCGGGACAGC13970.17121105163048808No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13670.1675343647665549No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13650.16728925230895936No Hit
GTCCCGGATGTTGCTGGTACCAAGCTAAGTAGTTCTTGTTTATGGACTTG13190.16165166578426182No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13030.15969076612349747No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12990.15920054120830637No Hit
GACCGGTTCAGTGCCAGCGGGTCTGGGACGGATTTCACTCTCACCATCAG12940.1585877600643175No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12850.15748475400513756No Hit
TCTTCAGCCTGCAGGCGGCTGATGGTGAGAGTGAAATCCGTCCCAGACCCGCTGGCACTGAACCGGTCAGGGACC12840.15736219777633978No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12550.15380806714120437No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12040.147557699472518No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGGACAAGTCCATAAACAAGAACTA11970.14669980587093356No Hit
GAGTGAAATCCGTCCCAGACCCGCTGGCACTGAACCGGTCAGGGACCCCG11900.14584191226934917No Hit
CTTATTACTGTCAGCAGTACTTTCGTCCTCCTCCCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAA11870.14547424358295585No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11270.1381208698550895No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11260.13799831362629175No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG11220.13750808871110065No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10990.13468929544875188No Hit
CTACTTAGCTTGGTACCAGCAACATCCGGGACAGCCTCCTAAACTGCTCATTTATTGGGCATCTGCCCGGAGATC10980.1345667392199541No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10760.13187050218640312No Hit
GTACCAGCAACATCCGGGACAGCCTCCTAAACTGCTCATTTATTGGGCATCTGCCCGGAGATCCGGGGTCCCTGA10670.13076749612722316No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10670.13076749612722316No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10600.12990960252563877No Hit
GTCCAAAAGACTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA10490.12856148400886325No Hit
GAACCGGTCAGGGACCCCGGATCTCCGGGCAGATGCCCAATAAATGAGCAGTTTAGGAGGCTGTCCCGGATGTTG10400.12745847794968332No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10260.1257426907465145No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10040.12304645371296352No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9990.12243367256897465No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9980.12231111634017687No Hit
GGCTGAAGATGTGGCAACTTATTACTGTCAGCAGTACTTTCGTCCTCCTC9870.12096299782340138No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9750.11949232307782812No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9750.11949232307782812No Hit
GTCAGGGACCCCGGATCTCCGGGCAGATGCCCAATAAATGAGCAGTTTAGGAGGCTGTCCCGGATGTTGCTGGTA9520.11667352981547935No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9510.11655097358668157No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9470.11606074867149048No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9440.11569307998509717No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9430.11557052375629939No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9350.11459007392591722No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9250.11336451163793948No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9220.11299684295154616No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9220.11299684295154616No Hit
GTAATATATGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGCAGATAAA9170.1123840618075573No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9170.1123840618075573No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9110.11164872443477068No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9080.11128105574837736No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9050.11091338706198403No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8990.11017804968919741No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8900.10907504363001745No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8810.1079720375708375No Hit
CACCATCAGCCGCCTGCAGGCTGAAGATGTGGCAACTTATTACTGTCAGCAGTACTTTCGTCCTCCTCCCACTTT8720.10686903151165755No Hit
GTACTTTCGTCCTCCTCCCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCAT8700.106623919054062No Hit
ACTTAGCTTGGTACCAGCAACATCCGGGACAGCCTCCTAAACTGCTCATT8690.10650136282526422No Hit
GGTCTGGGACGGATTTCACTCTCACCATCAGCCGCCTGCAGGCTGAAGAT8660.10613369413887092No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8620.10564346922367983No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA8620.10564346922367983No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8560.1049081318508932No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8520.1044179069357021No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8440.10343745710531993No Hit
ACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGGACAAGTCCATAAACAAGAACTACTTAGCTTGGTACCAGCAA8360.10245700727493773No Hit
CGCTGGCACTGAACCGGTCAGGGACCCCGGATCTCCGGGCAGATGCCCAATAAATGAGCAGTTTAGGAGGCTGTC8310.10184422613094889No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA8210.10061866384297115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT100.009475658131.5878370
GTATCCC200.00764567351.25084735
GTACACG200.00794549850.7546126
CGTATGC707.2759576E-1249.32001541
CACGAGA2300.047.0911721
ACGAGAC2350.046.10053322
CGAGACT2400.045.1428723
CCCACGA2400.045.1207419
CGATAAA306.939869E-445.1207418
TATACAC2350.044.6352583
GGTATCA6000.044.5512731
TCGTATG704.474714E-1044.3076740
CTTATAC2400.043.705361
CGAGCCC2600.041.6473615
TACTATC350.001099649641.0927359
TCTCCGA2650.040.8590611
ATACACA2750.039.3732764
TTATACA2650.038.3053672
GAGCATC2500.038.1869633
GCCCACG2850.037.9964118
Produced by FastQC (version 0.11.5)