FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815952
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTATTGGGCATCTGCCCGGAGATCCGGGGTCCCT53670.6577592799576446No Hit
GTTTAGGAGGCTGTCCCGGATGTTGCTGGTACCAAGCTAAGTAGTTCTTGTTTATGGACTTGTCCAAAAGACT44120.5407180814557719No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40890.5011324195540914No Hit
GTTCAGTGCCAGCGGGTCTGGGACGGATTTCACTCTCACCATCAGCCGCC30990.3798017530442967No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30830.37784085338353235No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28410.34818224601447145No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25760.31570484538306176No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24820.3041845598760711No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24580.3012432103849246No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21540.2639861168304018No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21120.2588387552208954No Hit
GTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGTG20540.2517304939506246No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20240.2480538070866914No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19190.23518540306292526No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGGA19180.2350628468341275No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18540.22721924819107006No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18210.22317489264074356No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18190.22292978018314805No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18130.22219444281036138No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18120.22207188658156363No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18000.22060121183599038No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17770.21778241857364158No Hit
CTTTTGGACAAGTCCATAAACAAGAACTACTTAGCTTGGTACCAGCAACA17660.2164343000568661No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCC17440.2137380630233151No Hit
GTCCCTGACCGGTTCAGTGCCAGCGGGTCTGGGACGGATTTCACTCTCAC17410.21337039433692176No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17400.213247838108124No Hit
ACCTTGGTCCCTCCGCCGAAAGTGGGAGGAGGACGAAAGTACTGCTGACAGTAATAAGTTGCCACATCTTCAGC17390.21312528187932625No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16810.20601702060905544No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16220.19878620310998688No Hit
GATTTCACTCTCACCATCAGCCGCCTGCAGGCTGAAGATGTGGCAACTTATTACTGTCAGCAGTACTTTCGTCCT15500.18996215463654723No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA15270.18714336137419849No Hit
GACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15230.18665313645900738No Hit
GCACTGAACCGGTCAGGGACCCCGGATCTCCGGGCAGATGCCCAATAAAT15220.1865305802302096No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14760.1808929937055121No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT14480.17746141929917447No Hit
GTAATAAGTTGCCACATCTTCAGCCTGCAGGCGGCTGATGGTGAGAGTGAAATCCGTCCCAGACCCGCTGGCA14120.17304939506245465No Hit
GTGCCAGCGGGTCTGGGACGGATTTCACTCTCACCATCAGCCGCCTGCAGGCTGAAGATGTGGCAACTTATTA13980.17133360785928584No Hit
ACTTATTACTGTCAGCAGTACTTTCGTCCTCCTCCCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAA13470.16508324019059945No Hit
CAGTAATAAGTTGCCACATCTTCAGCCTGCAGGCGGCTGATGGTGAGAGTGAAATCCGTCCCAGACCCGCTGGCA13110.16067121595387965No Hit
GTCCATAAACAAGAACTACTTAGCTTGGTACCAGCAACATCCGGGACAGC13010.15944565366590194No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12900.15809753514912642No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12780.15662686040355314No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGGACAAGTCCATAAACAAGAACTA12760.1563817479459576No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12760.1563817479459576No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12680.15540129811557543No Hit
ATCCGGGACAGCCTCCTAAACTGCTCATTTATTGGGCATCTGCCCGGAGATCCGGGGTCCCTGACCGGTTCAGTG12570.1540531795987999No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12560.15393062337000216No Hit
GTCCCGGATGTTGCTGGTACCAAGCTAAGTAGTTCTTGTTTATGGACTTG12240.15000882404847343No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12090.14817048061650684No Hit
GAGTGAAATCCGTCCCAGACCCGCTGGCACTGAACCGGTCAGGGACCCCG12030.14743514324372023No Hit
GACCGGTTCAGTGCCAGCGGGTCTGGGACGGATTTCACTCTCACCATCAG11920.14608702472694474No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11900.14584191226934917No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11820.144861462438967No Hit
TCTTCAGCCTGCAGGCGGCTGATGGTGAGAGTGAAATCCGTCCCAGACCCGCTGGCACTGAACCGGTCAGGGACC11360.13922387591426946No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11250.13787575739749397No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG11170.13689530756711177No Hit
GGCTGAAGATGTGGCAACTTATTACTGTCAGCAGTACTTTCGTCCTCCTC11160.136772751338314No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11060.1355471890503363No Hit
CTTATTACTGTCAGCAGTACTTTCGTCCTCCTCCCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAA11000.13481185167754967No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10870.1332186207031786No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10600.12990960252563877No Hit
CTACTTAGCTTGGTACCAGCAACATCCGGGACAGCCTCCTAAACTGCTCATTTATTGGGCATCTGCCCGGAGATC10550.1292968213816499No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG10480.1284389277800655No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG10450.12807125909367217No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10360.12696825303449225No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10330.1266005843480989No Hit
GTACCAGCAACATCCGGGACAGCCTCCTAAACTGCTCATTTATTGGGCATCTGCCCGGAGATCCGGGGTCCCTGA10180.12476224091613232No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10010.12267878502657019No Hit
GAACCGGTCAGGGACCCCGGATCTCCGGGCAGATGCCCAATAAATGAGCAGTTTAGGAGGCTGTCCCGGATGTTG9870.12096299782340138No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9830.12047277290821029No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9800.12010510422181697No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9770.11973743553542364No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9700.11887954193383925No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9610.11777653587465929No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9580.11740886718826597No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA9200.11275173049395064No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8960.1098103810028041No Hit
GTCCAAAAGACTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA8790.10772692511324196No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8730.10699158774045532No Hit
GGTCTGGGACGGATTTCACTCTCACCATCAGCCGCCTGCAGGCTGAAGAT8720.10686903151165755No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8700.106623919054062No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8680.10637880659646647No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8520.1044179069357021No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT8510.10429535070690434No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8500.10417279447810655No Hit
CACCATCAGCCGCCTGCAGGCTGAAGATGTGGCAACTTATTACTGTCAGCAGTACTTTCGTCCTCCTCCCACTTT8480.10392768202051102No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8470.10380512579171325No Hit
GTAATATATGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGCAGATAAA8400.10294723219012882No Hit
GTCAGGGACCCCGGATCTCCGGGCAGATGCCCAATAAATGAGCAGTTTAGGAGGCTGTCCCGGATGTTGCTGGTA8380.10270211973253329No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8270.1013540012157578No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8240.10098633252936447No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8200.10049610761417337No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA8170.10012843892778006No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8160.10000588269898228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)