FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004687805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727146
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAGTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGATGTCATGCGAACTGTGGCTGCACCAT47390.6517260632665242No Hit
CTCTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC35890.4935735051832782No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33180.4563045110610524No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30520.4197231367565798No Hit
GATCTATGGTGCCTCCAATTTGGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA23460.32263121848982185No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22570.3103915857338141No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18490.2542818086051495No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18220.2505686615892819No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18050.2482307542089209No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA17550.24135455603138847No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGACTATTAGTACGTGGTTGGCCTGGTATCAGCAGAAA17300.23791645694262223No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17130.23557854956226124No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16150.22210120113429765No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16040.2205884375352405No Hit
CCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC15970.21962576979038598No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15870.2182505301548795No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15380.21151185594089772No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15290.21027414026894187No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15230.20944899648763798No Hit
GTCCTGATCTATGGTGCCTCCAATTTGGAGAGTGGGGTCCCATCAAGGTT15210.20917394856053667No Hit
GACTATTAGTACGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGGTGCCTC15090.20752366099792888No Hit
CCTTCCACCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGCCG14950.2055983255082198No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT14430.19844707940358608No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14010.19267107293445884No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13330.18331944341301473No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13030.17919372450649526No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12730.1750680055999758No Hit
CAGTATAAGAGTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGATGTCATGCGAACTGTGGCTGCACCAT12220.16805428345889273No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11640.1600778935729551No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11520.1584276060103473No Hit
CTATTAGTACGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT11350.15608969862998628No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGACTATTAGT11220.15430188710382783No Hit
GGACCAAGGTGGATGTCATGCGAACTGTGGCTGCACCATCTGTCTTCATC11210.1541643631402772No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT11140.15320169539542267No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11040.15182645575991616No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10940.15045121612440968No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC10740.1477007368533967No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10360.14247482623847205No Hit
GTGCAGCCACAGTTCGCATGACATCCACCTTGGTCCCTTGGCCGAACGTC10280.14137463453006688No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10270.14123711056651622No Hit
GTAATAAACGGCCGAGTCCGCGGCGGTCACAGACCTCAGGTTCAGGGAGA10260.14109958660296557No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10220.14054949074876297No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10190.14013691885811103No Hit
GAGTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGATGTCATGCGAACTGTGGCTGCACCATCTGTCTTCAT10030.13793653544130066No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9890.13601119995159155No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9830.13518605617028767No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCATGACATCCACCTTGGTCCCTTGGCCGAACGTCCGAGAATAACT9670.1329856727534773No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC9630.1324355768992747No Hit
GTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGA9630.1324355768992747No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACGTACTA9550.1313353851908695No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9450.12996014555536303No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGAATAACTCTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGG9440.12982262159181238No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9420.1295475736647111No Hit
ACTTATTACTGCCAACAGTATAAGAGTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGATGTCATGCGAA9010.12390909115913447No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8960.12322147134138123No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8960.12322147134138123No Hit
GTCTCCTTCCACCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCACTT8960.12322147134138123No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8850.12170870774232409No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8810.12115861188812152No Hit
ACATCCACCTTGGTCCCTTGGCCGAACGTCCGAGAATAACTCTTATACTG8770.12060851603391892No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8540.117445464872254No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8320.11441993767413973No Hit
CTAATAGTCTCACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA8290.11400736578348776No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8290.11400736578348776No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8280.11386984181993712No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8150.11208203029377868No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8080.11111936254892414No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8050.11070679065827221No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8010.1101566948040696No Hit
CTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC7980.10974412291341767No Hit
CCCCTAAGGTCCTGATCTATGGTGCCTCCAATTTGGAGAGTGGGGTCCCA7960.10946907498631638No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7930.10905650309566443No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7890.10850640724146184No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGGTGCCTCCAATTTGGAGAGTGGGGTCCCAT7700.10589345193399949No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7670.10548088004334755No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7660.10534335607979692No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7640.10506830815269562No Hit
GTTTATTACTGTGCGAGAGTGCGGCGCGGTAGGGATCTCCAAGGCATTTC7610.10465573626204365No Hit
ATTAGTACGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA7580.10424316437139172No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7560.10396811644429041No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7520.10341802059008781No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7500.10314297266298651No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7500.10314297266298651No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7430.10218030491813197No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7310.10053001735552419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)