FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727146
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAGTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGATGTCATGCGAACTGTGGCTGCACCAT43740.6015298165705374No Hit
CTCTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC38840.5341430744307195No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32850.45176622026388097No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26000.35756230523168664No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24220.3330830397196712No Hit
GATCTATGGTGCCTCCAATTTGGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA22930.31534244842163744No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22170.30489062719178817No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19070.2622581984910871No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18260.25111875744348455No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16890.23227797443704565No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGACTATTAGTACGTGGTTGGCCTGGTATCAGCAGAAA16170.22237624906139894No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA16170.22237624906139894No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16130.22182615320719634No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15240.20958652045118864No Hit
GTCCTGATCTATGGTGCCTCCAATTTGGAGAGTGGGGTCCCATCAAGGTT15030.206698517216625No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14560.20023489092974453No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14440.19858460336713674No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14350.19734688769518088No Hit
GACTATTAGTACGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGGTGCCTC14210.1954215522054718No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14180.19500898031481986No Hit
CCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC14070.19349621671576273No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13890.19102078537185105No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13600.18703259042888223No Hit
CCTTCCACCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGCCG13290.18276934755881213No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13060.1796062963971472No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12840.17658076919903293No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11880.16337846869817066No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11830.1626908488804174No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11720.16117808528136027No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11570.15911522582810056No Hit
CAGTATAAGAGTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGATGTCATGCGAACTGTGGCTGCACCAT11350.15608969862998628No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA11220.15430188710382783No Hit
GTGCAGCCACAGTTCGCATGACATCCACCTTGGTCCCTTGGCCGAACGTC10820.1488009285618019No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10740.1477007368533967No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10720.1474256889262954No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCATGACATCCACCTTGGTCCCTTGGCCGAACGTCCGAGAATAACT10130.13931177507680714No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10100.1388992031861552No Hit
GAGTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGATGTCATGCGAACTGTGGCTGCACCATCTGTCTTCAT10050.13821158336840195No Hit
GTAATAAACGGCCGAGTCCGCGGCGGTCACAGACCTCAGGTTCAGGGAGA9820.13504853220673702No Hit
GGACCAAGGTGGATGTCATGCGAACTGTGGCTGCACCATCTGTCTTCATC9790.13463596031608507No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGACTATTAGT9770.1343609123889838No Hit
CTATTAGTACGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT9760.13422338842543313No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9720.13367329257123053No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9540.13119786122731886No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9450.12996014555536303No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9320.12817233402920458No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9180.1262469985394955No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9040.12432166304978644No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8970.1233589953049319No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGAATAACTCTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGG8950.1230839473778306No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8930.12280889945072929No Hit
GGTGGATGTCATGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC8920.12267137548717863No Hit
GTCTCCTTCCACCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCACTT8820.12129613585167215No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8750.12033346810681761No Hit
CTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC8710.11978337225261501No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACGTACTA8670.11923327639841241No Hit
ACTTATTACTGCCAACAGTATAAGAGTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGATGTCATGCGAA8640.11882070450776047No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8620.11854565658065917No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC8600.11827060865355787No Hit
ACATCCACCTTGGTCCCTTGGCCGAACGTCCGAGAATAACTCTTATACTG8430.11593270127319687No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8330.11455746163769036No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8310.11428241371058906No Hit
CTAATAGTCTCACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA8220.11304469803863322No Hit
GTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGA8170.11235707822087998No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8050.11070679065827221No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7990.1098816468769683No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7990.1098816468769683No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7910.10878145516856313No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7830.10768126346015792No Hit
GTTTATTACTGTGCGAGAGTGCGGCGCGGTAGGGATCTCCAAGGCATTTC7800.107268691569506No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7780.1069936436424047No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7700.10589345193399949No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7570.10410564040784108No Hit
CCCCTAAGGTCCTGATCTATGGTGCCTCCAATTTGGAGAGTGGGGTCCCA7490.10300544869943587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)