FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306117
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11950.3903736153170193No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT10510.34333277799011486No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGG7390.241410963781822No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6670.21789054511836978No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC6540.21364380285969092No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT6320.2064570082680805No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC5870.19175674660342287No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5330.1741164326058337No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC4960.16202955079267078No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG4260.13916247709209223No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC4200.13720244220347122No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT4160.13589575227772388No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCC4100.13393571738910284No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC3950.12903563016755032No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG3830.12511556039030827No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT3740.12217550805737676No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT3450.11270200609570852No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG3360.10976195376277698No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA3220.10518853902266127No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA3210.10486186654122442No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA3210.10486186654122442No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT3130.10224848668972973No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA3100.10126846924541923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC150.001647981775.4862164
AGGGGGG1600.055.0115170
CCCTACA350.006260304753.88883270
GCGGTAG200.00807537650.53912731
CGTGTCT200.00841800150.01069612
AGAGTCG307.46059E-444.45395711
GCTTGCG503.1504442E-642.59739348
GCCCCAT755.8951548E-541.91353670
TTGCGTT350.001117926240.9464350
TTATACA2200.040.9111632
CGTATGC2050.040.5980341
CTCGTAT1800.040.1365239
GCCGTCT1850.040.06485446
TATACAC2250.040.002023
TCGTATG2100.039.47044840
GGTATCA8850.038.796691
TCTCGTA1900.037.88993538
GACTGCA2300.037.7694426
ACGAGAC2300.037.72003622
CGAGACT2400.037.54482723
Produced by FastQC (version 0.11.5)