FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356319
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGGTCATCTATGAGGACAAGTACCGACCATCCGGGATCCCT81150.5983105744297618No Hit
GTACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT78400.57803510825993No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC76620.564911351975457No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45710.33701511222654845No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42150.3107675996576027No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCGGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC36160.26660394789131464No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACG35010.25812511658393045No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34840.2568717241297954No Hit
GTACTGGTCATCTATGAGGACAAGTACCGACCATCCGGGATCCCTGAGAG34420.25377510747840293No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32620.24050389325814947No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32390.23880812699667264No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31960.23563778137738983No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGGGGCGATTCCCACTGCTGTGTGTTGAGTAACAGTAGTAGT31930.23541659447371896No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31220.23018183775350784No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG30870.22760132387734744No Hit
GCCCAAGGCCGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC30720.22649538935899297No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGGGGCGATTCCCACTGCTGTGTGTTGAGTAA30330.22361995961127137No Hit
GTACTTGTCCTCATAGATGACCAGTACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTTGA30330.22361995961127137No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30280.2232513147718199No Hit
GACAAGTACCGACCATCCGGGATCCCTGAGAGATTCTCTGGCTCCACTTT29490.21742672630848645No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGG28750.21197078268460445No Hit
GACTACTACTGTTACTCAACACACAGCAGTGGGAATCGCCCCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT28640.21115976403781114No Hit
ACACACAGCAGTGGGAATCGCCCCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCCGCCCC27850.20533517557447767No Hit
CTACTACTGTTACTCAACACACAGCAGTGGGAATCGCCCCTTCGGCGGAG27820.2051139886708068No Hit
CTTTAGGGACCATGGCCACCTTGACTATCAGTGGGGCCCAGTTGGAGGATGAAGCTGACTACTACTGTTACTCAA27610.20356568034511055No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27550.20312330653776875No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27390.201943643051524No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCGGCCTTGGG27020.19921567123958303No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26620.19626651252397112No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26590.19604532562030025No Hit
CAGTAGTAGTCAGCTTCATCCTCCAACTGGGCCCCACTGATAGTCAAGGT26230.19339108277624953No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC25170.18557581217987804No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGGATTGTCAAAA24980.1841749617899624No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24860.18329021417527883No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24590.1812995320422408No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24140.17798172848717744No Hit
GTAGTAGTCAGCTTCATCCTCCAACTGGGCCCCACTGATAGTCAAGGTGG24040.17724443880827445No Hit
GCTGTGTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCCAACTGGGCCCCACTGATAGTCAAGGTGGCCATGGT23580.1738529062853208No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCGGCCTTGGGCTGACGTAGGACGG23350.17215714002384394No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23330.17200968208806336No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23250.17141985034494098No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23180.17090374756970889No Hit
TCCTACGTCAGCCCAAGGCCGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21710.16006558928983522No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21460.15822236509257778No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21100.1555681222485271No Hit
GCAGTGGGAATCGCCCCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCCGCCCCCTCGGTCA20920.15424100082650172No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20890.15401981392283084No Hit
GTCCTACGTCAGCCCAAGGCCGCCCCCTCGGTCACTCTGTTCCCACCCTC20790.15328252424392788No Hit
GACCAGTACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATT20450.15077573933565774No Hit
GGCCAGGCCCCTGTACTGGTCATCTATGAGGACAAGTACCGACCATCCGG20410.15048082346409658No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20050.14782658062004586No Hit
GTACCGACCATCCGGGATCCCTGAGAGATTCTCTGGCTCCACTTTAGGGA19980.1473104778448138No Hit
GAGTGACCGAGGGGGCGGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGGGGCGATTCCCA19930.1469418330053623No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19820.14613081435856903No Hit
GCTTGGTCCCTCCGCCGAAGGGGCGATTCCCACTGCTGTGTGTTGAGTAA19170.14133843144569974No Hit
GAGTAACAGTAGTAGTCAGCTTCATCCTCCAACTGGGCCCCACTGATAGT19120.14096978660624823No Hit
CTGATAGTCAAGGTGGCCATGGTCCCTAAAGTGGAGCCAGAGAATCTCTC19020.14023249692734527No Hit
CCCTAAAGTGGAGCCAGAGAATCTCTCAGGGATCCCGGATGGTCGGTACT18460.13610367472548862No Hit
GGATCACCTGCTCTGGAGATGGATTGTCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCC18430.13588248782181772No Hit
ACTCAACACACAGCAGTGGGAATCGCCCCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCCG18360.13536638504658566No Hit
GCTCTGGAGATGGATTGTCAAAAAAATATGCTTATTGGTACCAGCAGAAG17800.131237562844729No Hit
GACTATCAGTGGGGCCCAGTTGGAGGATGAAGCTGACTACTACTGTTACTCAACACACAGCAGTGGGAATCGCCC17740.1307951890373872No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC17360.1279934882575559No Hit
CAGCAGTGGGAATCGCCCCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCCGCCCCCTCGGT17250.12718246961076266No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17020.12548670334928583No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16980.12519178747772464No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG16900.12460195573460225No Hit
GTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCCAACTGGGCCCCACTG16650.12275873153734483No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16440.12121042321164859No Hit
ACTTTAGGGACCATGGCCACCTTGACTATCAGTGGGGCCCAGTTGGAGGA16310.12025194662907474No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16090.11862990933548818No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16010.1180400775923658No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC15840.11678668513823076No Hit
GGAGATGGATTGTCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGG15680.115607021651986No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15540.11457481610152183No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15510.11435362919785094No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA15320.1129527788079353No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCCGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG15010.1106671808033361No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15000.11059345183544579No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14990.11051972286755549No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14910.10992989112443312No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14900.10985616215654281No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG14770.10889768557396896No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGGGGCGATTCCCACTGC14630.1078654800235048No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14590.1075705641519436No Hit
CTGTTACTCAACACACAGCAGTGGGAATCGCCCCTTCGGCGGAGGGACCA14350.10580106892257646No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC14220.10484259234000261No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13930.102704452271184No Hit
GGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTTGACAATCCATCTCCAGAGCAGGTGATCCTGGC13880.10233580743173251No Hit
GTCCCTAAAGTGGAGCCAGAGAATCTCTCAGGGATCCCGGATGGTCGGTA13800.10174597568861012No Hit
GGTCATCTATGAGGACAAGTACCGACCATCCGGGATCCCTGAGAGATTCTCTGGCTCCACTTTAGGGACCATGGC13700.10100868600970715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)