FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356319
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC74780.5513452218836424No Hit
CCCCTGTACTGGTCATCTATGAGGACAAGTACCGACCATCCGGGATCCCT74130.5465528389707731No Hit
GTACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT73940.5451519885808574No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50770.3743219699790389No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49770.36694907319000913No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36340.26793106931334No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCGGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC33510.24706577140038588No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33050.24367423887743223No Hit
GTACTGGTCATCTATGAGGACAAGTACCGACCATCCGGGATCCCTGAGAG32860.24227338848751656No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32170.23718608970308605No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACG31460.23195133298287499No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29890.22037588502409833No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29860.2201546981204274No Hit
GTACTTGTCCTCATAGATGACCAGTACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTTGA29430.21698435250114465No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGGGGCGATTCCCACTGCTGTGTGTTGAGTAACAGTAGTAGT29380.21661570766169316No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28620.2110123061020306No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGGGGCGATTCCCACTGCTGTGTGTTGAGTAA28560.2105699322946888No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28420.2095377267442246No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG28290.20857925016165077No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGG27420.2021648299551949No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27360.20172245614785314No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCGGCCTTGGGCTGACGTAGGACGG27300.20128008234051137No Hit
GCCCAAGGCCGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC26620.19626651252397112No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26200.19316989587257863No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25700.18948344747806378No Hit
GACTACTACTGTTACTCAACACACAGCAGTGGGAATCGCCCCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT25190.18572327011565862No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCGGCCTTGGG25020.18446987766152356No Hit
GACAAGTACCGACCATCCGGGATCCCTGAGAGATTCTCTGGCTCCACTTT24820.18299529830371764No Hit
ACACACAGCAGTGGGAATCGCCCCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCCGCCCC24390.17982495268443485No Hit
CTTTAGGGACCATGGCCACCTTGACTATCAGTGGGGCCCAGTTGGAGGATGAAGCTGACTACTACTGTTACTCAA24260.17886647610186102No Hit
CTACTACTGTTACTCAACACACAGCAGTGGGAATCGCCCCTTCGGCGGAG23470.17304188763852751No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23430.17274697176696632No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23000.16957662614768354No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22920.16898679440456116No Hit
GTAGTAGTCAGCTTCATCCTCCAACTGGGCCCCACTGATAGTCAAGGTGG22860.16854442059721939No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22830.16832323369354849No Hit
CAGTAGTAGTCAGCTTCATCCTCCAACTGGGCCCCACTGATAGTCAAGGT22710.1674384860788649No Hit
GCTGTGTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCCAACTGGGCCCCACTGATAGTCAAGGTGGCCATGGT22680.16721729917519404No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC22470.1656689908494978No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21930.16168762658342176No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGGATTGTCAAAA21040.1551257484411853No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20470.15092319727143835No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20080.14804776752371676No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20080.14804776752371676No Hit
GACCAGTACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATT19920.14686810403747202No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19810.14605708539067874No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19380.14288673977139596No Hit
GAGTAACAGTAGTAGTCAGCTTCATCCTCCAACTGGGCCCCACTGATAGT19310.1423706369961639No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC19310.1423706369961639No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19060.14052741279890643No Hit
GAGTGACCGAGGGGGCGGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGGGGCGATTCCCA18810.13868418860164902No Hit
GCAGTGGGAATCGCCCCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCCGCCCCCTCGGTCA18760.13831554376219754No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18550.1367672354365013No Hit
TCCTACGTCAGCCCAAGGCCGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18180.1340392636245603No Hit
GGATCACCTGCTCTGGAGATGGATTGTCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCC17840.1315324787162902No Hit
GTACCGACCATCCGGGATCCCTGAGAGATTCTCTGGCTCCACTTTAGGGA17670.13027908626215515No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17580.12961552555114247No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17570.12954179658325216No Hit
GCTTGGTCCCTCCGCCGAAGGGGCGATTCCCACTGCTGTGTGTTGAGTAA17030.12556043231717612No Hit
CTGATAGTCAAGGTGGCCATGGTCCCTAAAGTGGAGCCAGAGAATCTCTC16940.12489687160616345No Hit
GGCCAGGCCCCTGTACTGGTCATCTATGAGGACAAGTACCGACCATCCGG16930.12482314263827314No Hit
GTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCCAACTGGGCCCCACTG16850.12423331089515077No Hit
GTCCTACGTCAGCCCAAGGCCGCCCCCTCGGTCACTCTGTTCCCACCCTC16810.12393839502358958No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG16680.12297991844101572No Hit
CCCTAAAGTGGAGCCAGAGAATCTCTCAGGGATCCCGGATGGTCGGTACT16620.12253754463367394No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC16370.12069432043641652No Hit
ACTCAACACACAGCAGTGGGAATCGCCCCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCCG16060.11840872243181728No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16000.1179663486244755No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA15890.11715532997768224No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15770.11627058236299867No Hit
GACTATCAGTGGGGCCCAGTTGGAGGATGAAGCTGACTACTACTGTTACTCAACACACAGCAGTGGGAATCGCCC15740.11604939545932777No Hit
CAGCAGTGGGAATCGCCCCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCCGCCCCCTCGGT15580.11486973197308303No Hit
GCTCTGGAGATGGATTGTCAAAAAAATATGCTTATTGGTACCAGCAGAAG15480.11413244229418004No Hit
GGTGGGAACAGAGTGACCGAGGGGGCGGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGGGG15240.11236294706481294No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15090.11125701254645846No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15060.11103582564278758No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15010.1106671808033361No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14710.10845531176662718No Hit
ACTTTAGGGACCATGGCCACCTTGACTATCAGTGGGGCCCAGTTGGAGGA14670.10816039589506597No Hit
GGAGATGGATTGTCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGG14230.10491632130789291No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14210.1047688633721123No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCCGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG14150.10432648956477052No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG14070.10373665782164815No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13880.10233580743173251No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGT200.00793728150.7697228
GGTATCA15100.043.9850731
TGGTATC4650.042.248272
AGGTGAT26600.039.79565470
CGTATCC350.001477794538.6973843
GTATCAA38350.038.4370571
GTGGTAT5350.038.003511
TATCAAC42600.034.587052
ATCAACG44250.033.209793
TCAACGC44750.032.9010474
CAACGCA46300.031.872715
AACGCAG47000.031.4700246
CGCAGAG50200.029.4639648
ACGCAGA50550.029.4608337
AGAGTAC51400.028.77078611
CAGAGTA51800.028.55177910
GCAGAGT52000.028.4440579
ACGAGGT2355.5766577E-827.02717670
GACAGCC3250.025.0238481
CAGGTGA28900.024.7233869
Produced by FastQC (version 0.11.5)