FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521486
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22570.43280164759936024No Hit
ATATAATAGGGATTCCCGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAACTGTGGCTGCACCAT21970.42129606547443266No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21340.4092152042432587No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15800.30298032928976043No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15250.29243354567524343No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14350.275175172487852No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14270.273641094871195No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13280.25465688436506445No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13170.25254752764216104No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12930.24794529479219002No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12740.2443018604526296No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12670.24295954253805474No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12600.24161722462347984No Hit
CTATTATATTGTTGACAGTAATAAGTTCCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCCGT12400.23778203058183728No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT12100.2320292395193735No Hit
GTCTGCATCTGTGGGAGACAGAGTCTCCATCGCTTGCCGGGCCAGTCAGA11350.21764726186321395No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11140.2136203081194893No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10820.20748399765286124No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCTCCATCGCTTGCCG9530.1827469960842669No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9350.1792953214467886No Hit
GACTATTGGTACGTCGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATCGTGCCTC9280.17795300353221372No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9230.17699420502180307No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9140.17526836770306392No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8800.16874853783227164No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8470.16242046766356144No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8400.16107814974898654No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGGAACTTATTACTGTCAACAATATAATAGGGATTCCCGGACGTT8300.1591605527281653No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8250.15820175421775465No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7810.14976432732614106No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGGAACTTA7710.1478467303053198No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7640.1465044123907449No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7540.14458681536992363No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7510.14401153626367727No Hit
GTGCAGCCACAGTTCGTTTGAATTCCACCTTGGTCCCTTGGCCGAACGTC7360.14113514073244537No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7310.14017634222203473No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7250.13902578400954196No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7220.13845050490329558No Hit
CTATTGGTACGTCGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT7220.13845050490329558No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6980.13384827205332456No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6920.1326977138408318No Hit
GATCTATCGTGCCTCCAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGAGACGGAATTCA6870.13173891533042115No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6810.13058835711792838No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6800.13039659741584625No Hit
TCGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGAT6780.130013078011682No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6720.12886251979918925No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCGGGTGCCAGTTGTGACAT6720.12886251979918925No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC6700.128479000395025No Hit
CCAATAGTCTGACTGGCCCGGCAAGCGATGGAGACTCTGTCTCCCACAGA6660.12771196158669648No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6580.12617788397003946No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6540.12541084516171094No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6520.12502732575754671No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCGGGTGCCAGTTGTGACATCCAGATGACCCAGTCTCCTTCCACC6410.12291796903464332No Hit
GGACCAAGGTGGAATTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC6320.12119213171590415No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6170.11831573618467227No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAATTCCACCTTGGTCC6170.11831573618467227No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6170.11831573618467227No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6170.11831573618467227No Hit
GTGGGAGACAGAGTCTCCATCGCTTGCCGGGCCAGTCAGACTATTGGTACGTCGTTGGCCTGGTATCAGCAGAAA6070.116398139163851No Hit
ACCTTGATGGGACCCCACTTTCTAAACTGGAGGCACGATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG5990.11486406154719397No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5980.11467230184511186No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCTCCATCGCTT5930.11371350333470123No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5910.11332998393053698No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5900.11313822422845483No Hit
GAGTGAATTCCGTCTCAGACCCACTGCCACTGAACCTTGATGGGACCCCA5820.11160414661179784No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5770.11064534810138718No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5770.11064534810138718No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5730.10987830929305867No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT5710.10949478988889444No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5680.10891951078264804No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5670.10872775108056593No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT5630.10796071227223741No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5630.10796071227223741No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5570.10681015405974464No Hit
CAATATAATAGGGATTCCCGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAACTGTGGCTGCACCAT5470.10489255703892339No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5450.10450903763475913No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5440.104317277932677No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5430.10412551823059488No Hit
GTAATAAGTTCCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCCGTCTCAGACCCACTGCCA5370.10297496001810211No Hit
GTGATGGTGAGAGTGAATTCCGTCTCAGACCCACTGCCACTGAACCTTGA5250.1006738435931166No Hit
GCCAAGGGACCAAGGTGGAATTCAAACGAACTGTGGCTGCACCATCTGTC5250.1006738435931166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATT250.00714628652.12929569
CGTATGC2500.048.4373641
TCGTATG2500.048.32385340
TGAACGT305.349261E-447.5645557
GACTGTT556.2571885E-446.06844370
ATGCCGT2650.045.8887344
ATCTCGT2600.044.90965337
ACGAGAC2750.044.40552522
CTTATAC2850.044.033421
CGTCTTC2800.043.72260748
TATACAC2950.043.6821333
CCGTCTT2800.043.64056847
TCTCCGA2800.043.58329811
ATCTCCG2850.042.81867610
AGCCCAC2850.042.81867617
CCGAGCC2850.042.81867614
CGAGCCC2850.042.81867615
CTACATC2650.042.55140333
CTCTCTA2800.042.531729
TGCTTGA2850.042.3611255
Produced by FastQC (version 0.11.5)