FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521486
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24920.4778651775886601No Hit
ATATAATAGGGATTCCCGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAACTGTGGCTGCACCAT21880.41957022815569356No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21090.4044212116912055No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17690.3392229129832824No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16420.31486943081885227No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15530.29780281733354297No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13860.2657789470858278No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12860.2466029768776151No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12650.24257602313389046No Hit
CTATTATATTGTTGACAGTAATAAGTTCCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCCGT12330.2364397126672624No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12320.23624795296518028No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12200.23394683654019474No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12030.2306869216047986No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11780.22589292905274544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11550.2214824559048565No Hit
GTCTGCATCTGTGGGAGACAGAGTCTCCATCGCTTGCCGGGCCAGTCAGA11490.22033189769236375No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10390.1992383304633298No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9880.18945858565714133No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9840.1886915468488128No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCTCCATCGCTTGCCG9540.182938755786349No Hit
GACTATTGGTACGTCGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATCGTGCCTC9330.17891180204262436No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9010.17277549157599628No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8760.16798149902394313No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8400.16107814974898654No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8280.15877703332400103No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8270.1585852736219189No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7990.15321600196361937No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGGAACTTATTACTGTCAACAATATAATAGGGATTCCCGGACGTT7770.14899728851781258No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7660.14688793179490917No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGGAACTTA7580.14535385417825214No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7580.14535385417825214No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7560.14497033477408788No Hit
GTGCAGCCACAGTTCGTTTGAATTCCACCTTGGTCCCTTGGCCGAACGTC7560.14497033477408788No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7290.13979282281787048No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7250.13902578400954196No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7160.13729994669080284No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7110.13634114818039217No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7070.13557410937206368No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6870.13173891533042115No Hit
GTGGGAGACAGAGTCTCCATCGCTTGCCGGGCCAGTCAGACTATTGGTACGTCGTTGGCCTGGTATCAGCAGAAA6700.128479000395025No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6560.1257943645658752No Hit
CTATTGGTACGTCGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT6470.12406852724713606No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCGGGTGCCAGTTGTGACAT6400.12272620933256118No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6380.12234268992839692No Hit
GATCTATCGTGCCTCCAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGAGACGGAATTCA6300.12080861231173992No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6290.12061685260965778No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC6290.12061685260965778No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6250.11984981380132928No Hit
GAGTGAATTCCGTCTCAGACCCACTGCCACTGAACCTTGATGGGACCCCA6210.11908277499300077No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6160.11812397648259014No Hit
TCGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGAT6040.11582286005760462No Hit
CCAATAGTCTGACTGGCCCGGCAAGCGATGGAGACTCTGTCTCCCACAGA6000.11505582124927612No Hit
GGACCAAGGTGGAATTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC5950.11409702273886549No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCGGGTGCCAGTTGTGACATCCAGATGACCCAGTCTCCTTCCACC5870.11256294512220846No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCTCCATCGCTT5800.11122062720763358No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5750.11026182869722292No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5600.10738543316599104No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5580.10700191376182679No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5550.10642663465558039No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5410.10374199882643062No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5400.10355023912434849No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAATTCCACCTTGGTCC5380.10316671972018425No Hit
CTCCTGTATAACGTCTGGATTCACCTTTAGTGGTTACTCTATGGGCTGGGTCCGCCAGGCTCCGGGGAGGACT5310.10182440180560935No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5280.10124912269936298No Hit
CAATATAATAGGGATTCCCGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAACTGTGGCTGCACCAT5240.10048208389103447No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5240.10048208389103447No Hit
GTGATGGTGAGAGTGAATTCCGTCTCAGACCCACTGCCACTGAACCTTGA5240.10048208389103447No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC5230.10029032418895234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGG200.007436330451.60930639
TTATGCG200.007485213751.5238638
GTATCAA12200.035.526481
GGCTTAT400.002699798734.2225935
ACTATGA400.002836043533.8806922
GTGGTAT1450.032.6935461
TATCAAC13350.032.4505652
ATCAACG14300.030.2802333
TCAACGC14250.030.1433034
CAACGCA14650.029.0894095
AACGCAG14900.028.8283256
GTATTAG855.1141233E-627.8856741
CACCGAC1603.6379788E-1227.4805478
AGAGTAC15850.027.31383511
ACGCAGA15750.027.2725127
CGCAGAG15800.027.1862058
TGTTAGA6950.026.85074870
GTCGCTG1655.456968E-1226.6785011
GCACCGA1655.456968E-1226.6478027
TGCACCG1655.456968E-1226.6478026
Produced by FastQC (version 0.11.5)