FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087293
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTGTGAGGAGC54890.5048317242914283No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCACCGTCCCTCCGCCGAAGACCCAAGCACTGAGTCTCGTGTCCCAAGCG44260.4070659886525527No Hit
GTGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT32550.29936732784999076No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGTTGCGCTTGG29300.2694765808296384No Hit
GTCTTGAACGGCCCTCAGGGATCTCAGAGAGATTTGCTGCATCCAGGTCAGGTAACACAGCCTCCCTGACCATTA27890.2565085951992701No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27770.25540493684774945No Hit
GGTCAGCACCGTCCCTCCGCCGAAGACCCAAGCACTGAGTCTCGTGTCCC27380.2518180472053071No Hit
GTGTTACCTGACCTGGATGCAGCAAATCTCTCTGAGATCCCTGAGGGCCG25700.23636683028401728No Hit
GTTCAAGACTGCTATAAAAAAGGAGTTTGGGAGGGCGGCCCGGGCGCTGCTGCAGCCAAGCTGCTCCTTGGCCGC23020.2117184604333882No Hit
CTATTACTGTTGCGCTTGGGACACGAGACTCAGTGCTTGGGTCTTCGGCG23020.2117184604333882No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22860.21024691596469397No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGG22370.20574031102931775No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTACCTGACCTGGATGCAGCAAATCTCTCTGAGATCCCTGAGGGCCGTT22010.20242933597475568No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21550.19819864562725964No Hit
GACTATTACTGTTGCGCTTGGGACACGAGACTCAGTGCTTGGGTCTTCGG20140.18523065999689137No Hit
CCCAAGCACTGAGTCTCGTGTCCCAAGCGCAACAGTAATAGTCAGCCTCG19760.18173574188374247No Hit
AGGTAACACAGCCTCCCTGACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGTTGCGCTTGGGA19240.17695322236048608No Hit
CAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGA18980.1745619625988579No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18820.17309041813016363No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18560.17069915836853544No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18380.16904367084125438No Hit
GGCTTGAGACAGACCGCCACAGTCACCTGCATTGGGAACATCCACAATGTTGGCGGCCAAGGAGCAGCTTGGCTG18350.1687677562533742No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18340.16867578472408082No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18050.16600861037457246No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCACAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17720.1629735499078905No Hit
GTCCAAGGGCTTGAGACAGACCGCCACAGTCACCTGCATTGGGAACATCCACAATGTTGGCGGCCAAGGAGCAG17190.15809905885534073No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16900.15543188450583237No Hit
GTTCCCACCCTCCTGTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16790.15442019768360507No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16610.152764710156324No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16170.14871796286741476No Hit
GTGCTTGGGTCTTCGGCGGAGGGACGGTGCTGACCGTCCTCGGTCAGCCC16140.14844204827953458No Hit
GCATTGGGAACATCCACAATGTTGGCGGCCAAGGAGCAGCTTGGCTGCAGCAGCGCCCGGGCCGCCCTCCCAAA16040.14752233298660067No Hit
CCTGTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16000.1471544468694271No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15890.14614276004719978No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15730.1446712155785055No Hit
GCCCTCAGGGATCTCAGAGAGATTTGCTGCATCCAGGTCAGGTAACACAG15600.14347558569769142No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCACAGGA15140.1392448953501954No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15100.13887700923302182No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14690.13510617653199275No Hit
CCTCACAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCACCGTCCCTC14530.13363463206329848No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14120.1298637993622694No Hit
GACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGTTGCG12830.11799947208342185No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12690.11671187067331437No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12680.11661989914402096No Hit
ATCCAGGTCAGGTAACACAGCCTCCCTGACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGTTG12510.11505638314603331No Hit
AATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACT12350.11358483867733905No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12320.11330892408945886No Hit
CTCTGTTCCCACCCTCCTGTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12110.11137752197429765No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12040.11073372126924388No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11920.10963006291772319No Hit
GCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTC11770.10825048997832232No Hit
CCTGAGGGCCGTTCAAGACTGCTATAAAAAAGGAGTTTGGGAGGGCGGCCCGGGCGCTGCTGCAGCCAAGCTGCT11640.10705486009750821No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11450.10530740104093377No Hit
CTCCTGTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAA11420.1050314864530536No Hit
GTAATGGTCAGGGAGGCTGTGTTACCTGACCTGGATGCAGCAAATCTCTCTGAGATCCCTGAGGGCCGTTCAAGA11370.10457162880658664No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10930.10052488151767738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)