FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087293
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTGTGAGGAGC50800.4672153688104311No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCACCGTCCCTCCGCCGAAGACCCAAGCACTGAGTCTCGTGTCCCAAGCG41260.37947452986453517No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31790.292377491623693No Hit
GTGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT30750.2828124525771802No Hit
GTCTTGAACGGCCCTCAGGGATCTCAGAGAGATTTGCTGCATCCAGGTCAGGTAACACAGCCTCCCTGACCATTA28790.26478603283567537No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGTTGCGCTTGG27100.24924284438509214No Hit
GGTCAGCACCGTCCCTCCGCCGAAGACCCAAGCACTGAGTCTCGTGTCCC26210.2410573782779803No Hit
GTGTTACCTGACCTGGATGCAGCAAATCTCTCTGAGATCCCTGAGGGCCG25320.2328719121708684No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22730.20905128608387988No Hit
GTTCAAGACTGCTATAAAAAAGGAGTTTGGGAGGGCGGCCCGGGCGCTGCTGCAGCCAAGCTGCTCCTTGGCCGC21290.19580738586563143No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGG20650.18992120799085435No Hit
CTATTACTGTTGCGCTTGGGACACGAGACTCAGTGCTTGGGTCTTCGGCG20530.18881754963933364No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTACCTGACCTGGATGCAGCAAATCTCTCTGAGATCCCTGAGGGCCGTT20180.18559854611406493No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19800.18210362800091603No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19570.17998828282716803No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19540.17971236823928785No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18880.17364224730592398No Hit
GGCTTGAGACAGACCGCCACAGTCACCTGCATTGGGAACATCCACAATGTTGGCGGCCAAGGAGCAGCTTGGCTG18670.17171084519076274No Hit
GACTATTACTGTTGCGCTTGGGACACGAGACTCAGTGCTTGGGTCTTCGG18570.17079112989782883No Hit
GTGCTTGGGTCTTCGGCGGAGGGACGGTGCTGACCGTCCTCGGTCAGCCC18530.17042324378065526No Hit
AGGTAACACAGCCTCCCTGACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGTTGCGCTTGGGA17830.16398523673011783No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17680.16260566379071695No Hit
GTTCCCACCCTCCTGTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17190.15809905885534073No Hit
GTCCAAGGGCTTGAGACAGACCGCCACAGTCACCTGCATTGGGAACATCCACAATGTTGGCGGCCAAGGAGCAG17060.15690342897452664No Hit
CCCAAGCACTGAGTCTCGTGTCCCAAGCGCAACAGTAATAGTCAGCCTCG16880.1552479414472456No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16810.15460414074219184No Hit
GCATTGGGAACATCCACAATGTTGGCGGCCAAGGAGCAGCTTGGCTGCAGCAGCGCCCGGGCCGCCCTCCCAAA16680.15340851086137777No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16380.15064936498257597No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCACAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16290.14982162121893547No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16210.14908584898458832No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16010.1472464183987205No Hit
GCCCTCAGGGATCTCAGAGAGATTTGCTGCATCCAGGTCAGGTAACACAG16010.1472464183987205No Hit
CAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGA15870.14595881698861302No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15710.14448727251991875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15670.14411938640274516No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15610.14356755722698483No Hit
CCTGTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15330.14099235440676985No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14490.13326674594612492No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14250.13105942924308353No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14020.1289440840693355No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13960.12839225489357514No Hit
GTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCAC13760.1265528243077073No Hit
CCTCACAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCACCGTCCCTC13600.12508127983901302No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCACAGGA13570.12480536525113287No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12890.11855130125918222No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12770.11744764290766149No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCACCGTCCCTCCGCCGAAGACC12670.11652792761472758No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12220.11238920879652495No Hit
CTCTGTTCCCACCCTCCTGTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12070.11100963585712408No Hit
CTCCTGTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAA11640.10705486009750821No Hit
GCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTC11630.10696288856821483No Hit
GACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGTTGCG11610.10677894550962803No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11410.10493951492376019No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11410.10493951492376019No Hit
AATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACT11250.10346797045506592No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10990.10107671069343775No Hit
ATCCAGGTCAGGTAACACAGCCTCCCTGACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGTTG10930.10052488151767738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAG150.001796924773.872766
GTCTACG200.00790105150.827831
GGTATCA8000.043.203661
GTGGTAT2350.041.8158041
ATTGCCG400.002195458435.69959357
GTATCAA19200.033.532251
TGGTATC2850.033.2876852
AATGGTA7000.029.66335770
TATCAAC21750.029.2866022
ATCAACG22150.028.6047573
TCAACGC22400.028.1342494
CAACGCA22600.028.0351945
AACGCAG22950.027.6165316
TATATCG500.00844765427.1056845
CGCAGAG24350.025.8919148
AGAGTAC24500.025.6009311
TAACACC801.0810846E-425.411584
AAAACCG2401.13313945E-725.11816470
CAGAGTA25250.024.84050810
TATGACT1101.3492227E-624.6415314
Produced by FastQC (version 0.11.5)