FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575764
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC88000.5584592616660871No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAG85130.5402458743822045No Hit
GTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCC74980.4758326754514001No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT65040.41275216339502613No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTC62610.39733107241947396No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAGGAGGCTGTCGTCCCATGAAGTACAATAATAATCAG62100.394094547153No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG58350.3702965672524566No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCT55070.3494812674994479No Hit
GAATTATGTTTACTGGTATCAACAACTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATGATCAGCGACC50180.3184487017091392No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC45870.29109689014344786No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC45620.2895103581500783No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT44740.2839257655334175No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG41220.261587395066774No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG40070.254289347897274No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39000.24749899096565223No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG38350.24337400778289137No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT38010.24121632427190873No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA37510.23804326028516962No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC37170.235885576774187No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCAC35720.2266836912126435No Hit
TTATTGTACTTCATGGGACGACAGCCTCCTGCTTTTCGGCGGAGGGACCA35090.2226856305893522No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT35080.22262216930961742No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA35060.22249524675014787No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34830.22103563731624784No Hit
CCCCTGAGGGTCGCTGATCATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGATACCAGTAAA34510.2190048763647348No Hit
GTACAATAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAG33850.21481643190223915No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC33480.2124683645520522No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG33470.21240490327231742No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32310.20504339482308262No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG31870.2022510985147522No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31700.20117225675926087No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA31700.20117225675926087No Hit
GTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC30830.19565112542233482No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT30610.1942549772681696No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC30600.19419151598843482No Hit
CCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAGTCTGGCACCTCGGCCTCCCTGGCCATCAGTGGGCTCCGGT30590.19412805470870004No Hit
TTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGATACCA30370.19273190655453482No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA29310.18600501090264787No Hit
ACATAATTCCTTCCGATGTCGGCACTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACG28720.18226079539829568No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG28680.18200695027935657No Hit
GTGCCAGACTTGGAGGCGGAGAATCGGTCAGGGACCCCTGAGGGTCGCTG28300.17959542164943482No Hit
GATTATTATTGTACTTCATGGGACGACAGCCTCCTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAG27640.17540697718693915No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG27530.17470890310985657No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGC27340.17350313879489568No Hit
CTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAGGCGGAGAATCGGTC27180.17248775831913915No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26370.16734739466062176No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTATTGTACTTCATGGGACGACAGCCTCCTGCTTTT25680.16296856635892176No Hit
GCTTGGTCCCTCCGCCGAAAAGCAGGAGGCTGTCGTCCCATGAAGTACAA25480.16169934076422612No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT25450.16150895692502176No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC24580.15598782558809568No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24380.15471859999340004No Hit
CTATAGTAATGATCAGCGACCCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAGTCTGGCACCTCGGCCTCC24360.15459167743393046No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG24320.15433783231499132No Hit
CTCTTGTTCTGGAAGCAGTGCCGACATCGGAAGGAATTATGTTTACTGGTATCAACAACTCCCAGGAACGGCCCC23960.15205322624453915No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC23890.15160899728639568No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAGGAGG23650.1500859265727609No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC23650.1500859265727609No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23430.14868977841859568No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22790.14462825651556957No Hit
GGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC22730.1442474888371609No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC22720.1441840275574261No Hit
GTCCGAGGATGAGGCTGATTATTATTGTACTTCATGGGACGACAGCCTCCTGCTTTTCGGCGGAGGGACCAAG22570.14323210836140438No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22320.1416455763680348No Hit
GATCATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGA22310.14158211508830001No Hit
GGACCAAGCTGACCGTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG22290.14145519252883046No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22110.14031288949360438No Hit
ATAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG21500.13644175142978263No Hit
ACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAGGAGGCTGTCGTCCCATGAAGTACAATAATAATCAGC21400.13580713863243482No Hit
GTTGATACCAGTAAACATAATTCCTTCCGATGTCGGCACTGCTTCCAGAA21150.13422060663906524No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21040.13352253256198263No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC20980.13314176488357393No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20920.13276099720516524No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20760.13174561672940874No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20360.1292071655400174No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAGGCGGAGAATCGGTCAGGGACC20040.12717640458850438No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC20010.12698602074930002No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19980.12679563691009568No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT19800.1256533338748696No Hit
CATCTATAGTAATGATCAGCGACCCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAGTCTGGCACCTCGGCCTC19650.12470141467884786No Hit
GTAATGATCAGCGACCCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAG19130.12140142813263914No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG18890.11987835741900438No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18740.11892643822298263No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18480.11727644494987828No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGTTGATACCAGTAAACATAATTCCTT18330.11632452575385654No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC18200.11549952911730436No Hit
GTCAGGGACCCCTGAGGGTCGCTGATCATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGATA17950.11391299712393481No Hit
AGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG17880.11346876816579132No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT17700.11232646513056524No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC17470.11086685569666524No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGATACCAGTAAACATAATTCCTTCCGATGTCGGCA17310.10985147522090873No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17300.10978801394117393No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16950.10756686915045653No Hit
GCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCCGAGGTGCC16750.10629764355576088No Hit
GGATGAGGCTGATTATTATTGTACTTCATGGGACGACAGCCTCCTGCTTTTCGGCGGAGGGACCAAGCTGACCGT16680.10585341459761742No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16680.10585341459761742No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGTGCCGACATCGGAAGGAATTAT16610.10540918563947392No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16590.10528226308000437No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC16580.10521880180026959No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16580.10521880180026959No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG16570.1051553405205348No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGA16550.10502841796106524No Hit
GCCCCCAAACTCCTCATCTATAGTAATGATCAGCGACCCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAGT16540.10496495668133046No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT16500.10471111156239134No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC16270.10325150212849132No Hit
ACCCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAGTCTGGCACCTCGG16240.10306111828928698No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAG16240.10306111828928698No Hit
GTCCCTGACCGATTCTCCGCCTCCAAGTCTGGCACCTCGGCCTCCCTGGC15950.10122074117697828No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG15930.10109381861750871No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC15910.10096689605803916No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG15890.10083997349856959No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG15840.10052266709989566No Hit
GTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG15780.10014189942148698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)