FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575764
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC80890.5133382917746566No Hit
GTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCC79860.5068017799619741No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAG64510.4093887155690827No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG60990.3870503451024392No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT60040.38102152352763485No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCT55750.35379663452141313No Hit
GAATTATGTTTACTGGTATCAACAACTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATGATCAGCGACC55320.3510677994928174No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTC49910.3167352471563No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAGGAGGCTGTCGTCCCATGAAGTACAATAATAATCAG48110.30531221680403914No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42190.26774313920104786No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT40330.2559393411703783No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG40280.2556220347717044No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG39890.25314704486204787No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC39610.251370129029474No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA37940.24077209531376526No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT37120.2355682703755131No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36660.2326490515077131No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG36650.23258559022797828No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC35520.2254144656179479No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCAC35270.2238279336245783No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34690.22014717939996092No Hit
TTATTGTACTTCATGGGACGACAGCCTCCTGCTTTTCGGCGGAGGGACCA34080.21627604133613917No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA33770.2143087416643609No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG33670.2136741288670131No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33570.21303951606966523No Hit
CCCCTGAGGGTCGCTGATCATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGATACCAGTAAA33560.21297605478993045No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG33280.21119913895735654No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31860.20218763723501743No Hit
CCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAGTCTGGCACCTCGGCCTCCCTGGCCATCAGTGGGCTCCGGT31440.19952226348615656No Hit
GTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC31230.19818957661172612No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30960.19647612205888698No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG30330.19247806143559568No Hit
CTCTTGTTCTGGAAGCAGTGCCGACATCGGAAGGAATTATGTTTACTGGTATCAACAACTCCCAGGAACGGCCCC29580.187718465455487No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT29300.1859415496229131No Hit
TTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGATACCA29260.18568770450397393No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG29080.18454540146874784No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27830.1766127415019No Hit
GATTATTATTGTACTTCATGGGACGACAGCCTCCTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAG27290.17318583239622176No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27270.1730589098367522No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26980.1712185327244435No Hit
CTATAGTAATGATCAGCGACCCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAGTCTGGCACCTCGGCCTCC26730.16963200073107393No Hit
ACATAATTCCTTCCGATGTCGGCACTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACG26710.16950507817160437No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTATTGTACTTCATGGGACGACAGCCTCCTGCTTTT26660.16918777177293046No Hit
GTGCCAGACTTGGAGGCGGAGAATCGGTCAGGGACCCCTGAGGGTCGCTG26630.16899738793372612No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26180.1661416303456609No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25600.1624608761210435No Hit
CTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAGGCGGAGAATCGGTC24780.15725705118279132No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24740.1570032060638522No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC24700.15674936094491307No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24550.15579744174889135No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC24460.1552262902312783No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT24100.15294168416082612No Hit
GGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC23920.15179938112560004No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC23430.14868977841859568No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23310.1479282430617783No Hit
GTCCGAGGATGAGGCTGATTATTATTGTACTTCATGGGACGACAGCCTCCTGCTTTTCGGCGGAGGGACCAAG22960.1457070982710609No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22930.14551671443185654No Hit
GTAATGATCAGCGACCCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAG22890.1452628693129174No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGC21790.13828212854209132No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21730.13790136086368263No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT21720.13783789958394785No Hit
CATCTATAGTAATGATCAGCGACCCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAGTCTGGCACCTCGGCCTC21480.13631482887031307No Hit
GATCATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGA21090.13383983896065654No Hit
GTTGATACCAGTAAACATAATTCCTTCCGATGTCGGCACTGCTTCCAGAA21040.13352253256198263No Hit
GGACCAAGCTGACCGTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG21010.1333321487227783No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAGGAGG20850.13231676824702177No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20720.13149177161046957No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20690.13130138777126524No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20440.12971485577789568No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGTTGATACCAGTAAACATAATTCCTT20410.12952447193869132No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGTGCCGACATCGGAAGGAATTAT19910.12635140795195218No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19760.12539948875593046No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19600.12438410828017393No Hit
GCTTGGTCCCTCCGCCGAAAAGCAGGAGGCTGTCGTCCCATGAAGTACAA18660.11841874798510436No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC18610.11810144158643046No Hit
GTACAATAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAG18290.1160706806349174No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG18250.1158168355159783No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17990.11416684224287393No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17910.11365915200499568No Hit
AGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG17720.1124533876900348No Hit
GCCCCCAAACTCCTCATCTATAGTAATGATCAGCGACCCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAGT17720.1124533876900348No Hit
GTCAGGGACCCCTGAGGGTCGCTGATCATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGATA17620.11181877489268699No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC17560.11143800721427827No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17490.11099377825613481No Hit
GTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG17230.10934378498303045No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGTGCCGACATCGGAAGGAATTATGTTTA17170.10896301730462177No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC17150.1088360947451522No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG17050.10820148194780436No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16910.1073130240315174No Hit
GGATGAGGCTGATTATTATTGTACTTCATGGGACGACAGCCTCCTGCTTTTCGGCGGAGGGACCAAGCTGACCGT16870.10705917891257828No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC16780.10648802739496523No Hit
GTCCCTGACCGATTCTCCGCCTCCAAGTCTGGCACCTCGGCCTCCCTGGC16600.10534572435973914No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16580.10521880180026959No Hit
ACCCTCAGGGGTCCCTGACCGATTCTCCGCCTCCAAGTCTGGCACCTCGG16540.10496495668133046No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAAAG16290.10337842468796088No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGATACCAGTAAACATAATTCCTTCCGATGTCGGCA16240.10306111828928698No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16220.10293419572981741No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16120.10229958293246957No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16070.10198227653379567No Hit
GGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGTGCCGACATCGGAAGGAATTATGTTTACTGGTATCAACAA15980.10141112501618262No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGT15910.10096689605803916No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC15890.10083997349856959No Hit
CTCCTCATCTATAGTAATGATCAGCGACCCTCAGGGGTCCCTGACCGATT15860.10064958965936524No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15820.1003957445404261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)