FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821918
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC65670.36044432296074796No Hit
GTAGTAGACGGCCGTGTCCTCGGCTCTTAGGGCGTTCATTTGCAGATAAA65430.3591270298663277No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGACCAGGGAGG57170.3137901925333632No Hit
GATTATTACTGTGCAGCGTGGGATGCCAGCCTGAATGGTGTATTGCTCGG56230.30863079458021714No Hit
CTATTCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGGGACGATTCGACGAACACTCTTTATCTGCAAATGAA52020.28552327821559476No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT51340.281790947781404No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAGCAATACACCATTCAGGCTGGCATCCCACG48450.2659285434360932No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47620.2613729048178897No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC45790.2513285449729351No Hit
GTGTATTGCTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC45370.24902328205769964No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC42910.2355210278398918No Hit
GTCTACTACTGTGCAAAAGATCTCTTCCATGACTACGGTGGTAATTCCGG42720.2344781708068091No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT42540.23349020098599385No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAGCAATACACCATTCAGG35360.19408118257792062No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34820.191117273115475No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34690.19040373935599736No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT34160.18749471710581925No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33980.18650674728500405No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31710.17404735010027894No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31670.1738278012512089No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31610.17349847797760382No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC31390.17229095930771857No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30710.1685586288735278No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29830.16372855419398677No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29790.16350900534491672No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA29300.16081953194380866No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTC28900.1586240434531082No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28750.15780073526909555No Hit
GTCTGAATAGTGTGTGGTTTGAGGACTAGCTGAAATACCTGAGACCCACT28280.15522103629252249No Hit
GTCTGGTACCTCAGCCTCCCTGGTCATCAGTGGGCTCCGGTCCGAGGATG27780.1524766756791469No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27730.15220223961780938No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27610.15154359307059923No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27310.14989697670257388No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG27250.1495676534289688No Hit
GTCTGGGGGCCGTTCCTGGGAATTGTTGGTACCAGTGGACATAATAACTT27200.14929321736763124No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27120.14885411966949116No Hit
GCAATACACCATTCAGGCTGGCATCCCACGCTGCACAGTAATAATCACCC26940.14786614984867594No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC26300.14435336826355522No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC25460.13974284243308427No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24860.13644960969703357No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24820.13623006084796352No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC24810.136175173635696No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG24750.13584585036209093No Hit
GTACCAGACTTGGAGCCAGAGAAGCGGTCAGGGACCCCTGAGGGCCGGAA24400.13392479793272802No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23860.13096088847028242No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23370.12827141506917436No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA23320.1279969790078368No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23200.12733833246062667No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22870.1255270544557988No Hit
CCCCTGAGGGCCGGAAACTATTTAAATGAATGAGGAGTCTGGGGGCCGTTCCTGGGAATTGTTGGTACCAGTGGA22700.12459397184725109No Hit
GCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGACCAGGGAGGCTGAGGTACCAGACTTGGAG22580.12393532530004094No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21970.12058720535172275No Hit
TTACTGTGCAGCGTGGGATGCCAGCCTGAATGGTGTATTGCTCGGCGGAG21520.11811728079968473No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21480.11789773195061469No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT21100.11581201788444925No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20820.11427517594095893No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG20620.11317743169560869No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT20340.11164058975211837No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20270.11125637926624578No Hit
CTCCTGTGCCGCCTCTGGATTCACCATCGCCCCTTACGCCATGGGCTGGGCCCGCCAGGCTCCAGGGAAGGGG20260.11120149205397828No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20230.11103683041717574No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTTATTATGTCCACTGGTACCAACAATTCCCAGGAACGGCCCC20210.11092705599264072No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20150.11059773271903565No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20110.1103781838699656No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19970.10960976289822046No Hit
GCCCCCAGACTCCTCATTCATTTAAATAGTTTCCGGCCCTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAAGT19940.10944510126141792No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAGCAATACAC19800.10867668028967274No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19700.10812780816699763No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC19610.10763382325659004No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19220.1054932219781571No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18810.10324284627518912No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18660.10241953809117645No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18450.10126690663355871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.045.8822821
TCGTATG1600.045.28761340
CTACGCT4350.044.7418828
TACGCTG4450.043.76292429
ACGAGAC4700.041.33158522
ATCTCGT2000.041.25787737
CGTATGC2450.040.88566241
GTGGTAT4450.040.5846061
ACTACGC4800.040.52614627
CGAGACT4800.040.47384623
ATCGCGT1050.039.29321337
GCGTATG1150.039.00548640
TAGTACG350.00143390538.9362374
ATCTCCG5050.038.45118710
TCTCCGA5200.037.9971411
CGCGTAT1009.094947E-1237.9058139
TATACAC5400.037.8546753
TCTCGTA2150.036.8220938
CGAGCCC5400.035.95799315
CACGAGA5450.035.6408221
Produced by FastQC (version 0.11.5)