FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821918
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61500.3375563554451957No Hit
GTAGTAGACGGCCGTGTCCTCGGCTCTTAGGGCGTTCATTTGCAGATAAA58400.3205413196422671No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC56170.308301471306612No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55250.30325184777800096No Hit
GATTATTACTGTGCAGCGTGGGATGCCAGCCTGAATGGTGTATTGCTCGG50740.2784977150453533No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49630.27240523448365955No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT48930.2685631296249337No Hit
CTATTCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGGGACGATTCGACGAACACTCTTTATCTGCAAATGAA47780.26225110021416986No Hit
GTGTATTGCTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC44340.24336989919414592No Hit
GTCTACTACTGTGCAAAAGATCTCTTCCATGACTACGGTGGTAATTCCGG41480.22767215648563768No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG40110.2201526084049886No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT40050.2198232851313835No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAGCAATACACCATTCAGGCTGGCATCCCACG39580.2172435861548105No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT38260.20999847413549896No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC36840.20220448999351232No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34330.18842779971436693No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33780.18540900303965382No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32780.17992028181290268No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32040.17585862810510683No Hit
GTCTGGTACCTCAGCCTCCCTGGTCATCAGTGGGCTCCGGTCCGAGGATG32000.17563907925603675No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC30830.16921727542073794No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA30100.16521050892520958No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30050.16493607286387202No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA29340.1610390807928787No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29060.15950223884938838No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC28730.15769096084456052No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTC28680.15741652478322296No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28530.15659321659921027No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28410.15593457005200015No Hit
GTCTGGGGGCCGTTCCTGGGAATTGTTGGTACCAGTGGACATAATAACTT28090.15417817925943977No Hit
GTCTGAATAGTGTGTGGTTTGAGGACTAGCTGAAATACCTGAGACCCACT27500.1509398337356566No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG27440.15061051046205154No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27090.14868945803268863No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26810.1471526160891983No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26790.14704284166466328No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26090.14320073680593748No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAGCAATACACCATTCAGG25690.14100524831523703No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTTATTATGTCCACTGGTACCAACAATTCCCAGGAACGGCCCC25490.1399075040698868No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25030.13738269230558126No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC24810.136175173635696No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24200.13282705368737782No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23400.12843607670597687No Hit
GTACCAGACTTGGAGCCAGAGAAGCGGTCAGGGACCCCTGAGGGCCGGAA23000.12624058821527642No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG22680.12448419742271605No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC22420.12305712990376076No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22400.12294735547922572No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22260.12217893450748057No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22130.12146540074800292No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21950.12047743092718773No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC21830.11981878437997757No Hit
CCCCTGAGGGCCGGAAACTATTTAAATGAATGAGGAGTCTGGGGGCCGTTCCTGGGAATTGTTGGTACCAGTGGA21820.11976389716771008No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT21730.11926991225730246No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21550.11828194243648725No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21470.11784284473834716No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGACCAGGGAGG21440.11767818310154464No Hit
CTCCTGTGCCGCCTCTGGATTCACCATCGCCCCTTACGCCATGGGCTGGGCCCGCCAGGCTCCAGGGAAGGGG21300.11690976212979948No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC21090.11575713067218173No Hit
GCCCCCAGACTCCTCATTCATTTAAATAGTTTCCGGCCCTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAAGT20880.11460449921456399No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT20760.11394585266735387No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20620.11317743169560869No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20580.11295788284653865No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20050.11004886059636054No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19550.10730449998298495No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19500.10703006392164739No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19370.10631653016216974No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19110.10488946264321446No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18870.10357216954879418No Hit
CCTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAAGTCTGGTACCTCAGCCTCCCTGGTCATCAGTGGGCTCCGGT18730.102803748577049No Hit
TTACTGTGCAGCGTGGGATGCCAGCCTGAATGGTGTATTGCTCGGCGGAG18510.10159622990716377No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC18380.10088269614768612No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18380.10088269614768612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGT200.00567753455.25905267
GTGGTAT4950.044.0357321
GGTATCA17800.042.4779361
TGGTATC5350.040.723212
GTATCAA40550.035.2767751
GGCCAAG15700.033.8619570
GACAGCC3200.032.9945451
GACGGCC16150.031.4003247
AGTAGAC16500.030.940533
GTAGACG16500.030.9388314
TTTCACG703.572807E-430.87312969
AGACGGC16550.030.6414056
TAGACGG16600.030.549115
GCCGTGT16800.030.38718611
TATCAAC46650.030.3569052
AGAGTCG450.00494095430.252965
CCGTGTC16700.030.16072712
TAGTAGA17100.029.8614542
ATCAACG47250.029.8207763
GGCCGTG17050.029.74201610
Produced by FastQC (version 0.11.5)