FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427680
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60830.4260758713437185No Hit
GCATAATATTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAACTGTGGCTGCACCAT55610.38951305614703574No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54640.38271881654152196No Hit
ATATTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGA54380.3808976801524151No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCA54290.38026728678695504No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG48510.33978202398296536No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46470.3254931076992043No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39560.27709290597332736No Hit
ATATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCCGATTGTGAGAGTGAACTCTGTC39100.2738708954387538No Hit
GGTAAATATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCCGATTGTGAGAGTGAACT38960.272890283536927No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38140.2671466995405133No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC35390.24788468004034517No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34530.24186092121483804No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33310.23331558892749077No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32920.23058388434383054No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32430.22715174268743693No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32240.22582091224924353No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32140.22512047517651013No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31530.2208478090328365No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30000.2101311218200157No Hit
GAGTTCACTCTCACAATCGGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGCATAATATTTAC29500.20662893645634878No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28570.20011487167992825No Hit
ACTTATTACTGTCTACAGCATAATATTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAA28010.1961924240726213No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27790.19465146251260787No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26370.1847052560797938No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26300.18421495012888042No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25990.18204359520340693No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25880.18127311442340022No Hit
GTGCAGCCACAGTTCGTTTGAATTCCACCTTGGTCCCTTGGCCGAACGTC25330.17742071052336658No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25080.17566961784153312No Hit
GTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAACT23900.16740446038327916No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCCGATTGTGAGAGTGAACTCTGTCCCAGATCCAGTGCCG22420.15703799170682506No Hit
GTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGGGCATGAGCAATTATTTAGCCTGGTTTCAGCAGAAA22410.15696794799955172No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT22370.15668777317045834No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21740.15227501961223802No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21230.14870279054129779No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20980.1469516978594643No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20200.1414882886921439No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20170.1412781575703239No Hit
GTCTCCATCTGCCATGTCTGCATCTGTAGGAGACACAGTCACCATCACTT20040.14036758937577049No Hit
ATCTGGGACAGAGTTCACTCTCACAATCGGCAGCCTGCAGCCTGAAGATT19900.13938697747394374No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19630.13749579737756362No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19010.1331530875266166No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18470.12937072733385632No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18080.12663902275019615No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17670.12376723075198924No Hit
AGTCAGGGCATGAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGCGCCTGATCTATG17570.12306679367925587No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACATCC17530.12278661885016251No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17350.12152583211924241No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17300.12117561358287571No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCACTCTCA17250.12082539504650902No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17200.12047517651014232No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17130.11998487055922896No Hit
GCCCCTAAGCGCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGAT16990.1190042586574022No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16760.11739325339011543No Hit
GTCTACAGCATAATATTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAACTGTGGCTGCAC16610.11634259778101537No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16540.11585229183010198No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16370.11466154880645522No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGG16270.11396111173372185No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16180.1133307183682618No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16090.11270032500280175No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16040.11235010646643505No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15490.10849770256640143No Hit
GGACCAAGGTGGAATTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC15450.10821752773730808No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15440.10814748403003474No Hit
GAGTGAACTCTGTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCA15390.10779726549366805No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTG15350.10751709066457471No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15340.10744704695730137No Hit
CTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATAATTGCTCA15280.10702678471366132No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15260.10688669729911465No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15230.10667656617729462No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15150.10611621651910792No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14980.10492547349546116No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14830.10387481788636109No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCACT14790.10359464305726773No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14580.10212372520452763No Hit
CTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14520.10170346296088759No Hit
GGATCTGGGACAGAGTTCACTCTCACAATCGGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAG14420.10100302588815421No Hit
GTGTGACATCCAGATGACCCAGTCTCCATCTGCCATGTCTGCATCTGTAG14320.10030258881542083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)