FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427680
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60900.42656617729463187No Hit
ATATTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGA49920.3496581867085061No Hit
GCATAATATTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAACTGTGGCTGCACCAT48360.3387313683738653No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCA47920.33564944525383844No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46270.32409223355373756No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG45490.31862882438641715No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43440.3042698643953827No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40320.2824162277261011No Hit
ATATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCCGATTGTGAGAGTGAACTCTGTC38970.2729603272442004No Hit
GGTAAATATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCCGATTGTGAGAGTGAACT36350.25460887593858567No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36080.2527176958422055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34870.24424240726213156No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34620.2424913145802981No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30290.2121623893309425No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30020.21027120923456238No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29410.2059985430908887No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC29260.20494788748178866No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29260.20494788748178866No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28610.20039504650902162No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28220.19766334192536142No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28200.19752325451081473No Hit
GAGTTCACTCTCACAATCGGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGCATAATATTTAC27550.19297041353804775No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26600.1863162613470806No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26010.18218368261795362No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25720.1801524151070268No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCCGATTGTGAGAGTGAACTCTGTCCCAGATCCAGTGCCG25590.17924184691247338No Hit
ACTTATTACTGTCTACAGCATAATATTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAA24780.17356830662333295No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24710.1730780006724196No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24480.1714669954051328No Hit
GTGCAGCCACAGTTCGTTTGAATTCCACCTTGGTCCCTTGGCCGAACGTC24180.16936568418693265No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23940.16768463521237253No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23700.1660035862378124No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23380.16376218760506556No Hit
GTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAACT22270.155987336097725No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21860.1531155440995181No Hit
GTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGGGCATGAGCAATTATTTAGCCTGGTTTCAGCAGAAA20300.1421887257648773No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT20070.14057772049759049No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20070.14057772049759049No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18920.13252269416115658No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18790.13161212596660316No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCACT18690.13091168889386978No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18580.13014120811386307No Hit
GTCTCCATCTGCCATGTCTGCATCTGTAGGAGACACAGTCACCATCACTT18240.12775972206656955No Hit
ATCTGGGACAGAGTTCACTCTCACAATCGGCAGCCTGCAGCCTGAAGATT17910.12544827972654937No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17640.12355709963016923No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGG17210.12054522021741566No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17190.12040513280286899No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16930.11858399641376219No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16840.11795360304830214No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16740.11725316597556874No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16670.11676286002465538No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16300.11417124285554187No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16270.11396111173372185No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15810.11073910119914827No Hit
GTCTACAGCATAATATTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAACTGTGGCTGCAC15800.11066905749187493No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15660.10968844559004819No Hit
GAGTGAACTCTGTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCA15540.10884792110276813No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15380.10772722178639471No Hit
GCCCCTAAGCGCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGAT15340.10744704695730137No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15220.10660652247002131No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15190.10639639134820127No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACATCC15090.1056959542754679No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCACTCTCA15080.10562591056819455No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14960.10478538608091449No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14930.10457525495909449No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14780.10352459934999439No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14680.102824162277261No Hit
AGTCAGGGCATGAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGCGCCTGATCTATG14630.10247394374089433No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14510.10163341925361427No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14440.10114311330270088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT651.328172E-553.96627870
TACTTCG200.00667841153.03633554
GGTATCA14250.042.387521
GTATCAA40300.038.901811
CCTAACG350.00146717938.7543372
GTGGTAT4250.037.526831
GTACGTA909.709311E-734.5119469
TATCAAC45250.034.397152
ATCAACG45300.034.2699133
TCAACGC45750.033.999774
CAACGCA46850.033.200325
AACGCAG47400.032.743596
TGGTATC4950.031.5123622
ACGCAGA50450.030.965567
AATGGTA8400.030.90211970
CGCAGAG51050.030.4024758
AGAGTAC52900.029.6574711
CAGAGTA53400.029.25286710
TGGTACG903.761017E-629.01456867
GCAGAGT53650.028.9922669
Produced by FastQC (version 0.11.5)