FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129464
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGA51160.45295821734911423No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37470.33175028154947833No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33190.29385620081737884No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA28180.24949887734358953No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28010.247993738622922No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25720.2277186346798127No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25030.22160954222533877No Hit
GTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT24220.2144379989092171No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA23410.2072664555930955No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22050.19522534582775547No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21550.19079846723755692No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20750.18371546149323928No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT20620.18256447305978765No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20400.18061664648010028No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20150.17840320718500102No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19330.17114312629707543No Hit
GAGTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA18270.16175814368585453No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18250.16158106854224658No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTTCTTAACCTGGTACC17750.15715418995204805No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17440.15440952522612494No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17210.15237316107463364No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17020.15069094721035817No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16940.14998264663592642No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16560.14661821890737553No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG16080.14236841546078494No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16050.142102802745373No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15940.14112888945552934No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15570.13785299929878242No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT15550.1376759241551745No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15150.13413442128301567No Hit
GTAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA14710.13023876812364094No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG14460.1280253288285417No Hit
GTAATATATGGCGGTGTCCGAGGCCTGCAGGCTGCTCCAGTGCAGGTAGG14300.12660872767967815No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13830.12244746180489151No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGC13530.11979133465077241No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13520.11970279707896843No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13270.11748935778386915No Hit
GTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13080.11580714391959371No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13060.11563006877598578No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13040.11545299363237783No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12960.11474469305794606No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAACTCTGTC12750.11288540405006268No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12750.11288540405006268No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12680.11226564104743489No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12600.11155734047300313No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12400.1097865890369237No Hit
CATCTACGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA12320.10907828846249196No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12110.10721899945460855No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12070.10686484916739268No Hit
GTCAGAGTGTTAGCAGCAGCTTCTTAACCTGGTACCAGCAGAAACCTGGC11680.10341188386703783No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11330.10031306885389885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)