FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619458
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64950.40106010776444956No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54930.33918755534258993No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41570.25669081878011035No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC41150.2540973584989546No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41080.253665115118762No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40990.25310937362994285No Hit
GTCTATACCGCAGCACCGGGCCTCCGTGGACGTTCGGCCAAGGGACCACGGTGGACATCAAACGAACTGTGGCTG39530.2440940117002108No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGGCGTCAGAGGCCCTGTT37960.2343994101730332No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCGGCTACATGGCCTGGTATCAACAAAAGCCTGGCCAGCCTCCCAGGCT37140.22933598771934807No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCTATACCG36600.2260015387864335No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36030.2224818426905792No Hit
GTATCAGATACCACCGGAGAAATTGTGTTGCGGCAGTCTCCAGCCACCCT35820.2211851125500013No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34590.2135899788694736No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33300.20562435086306655No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33080.2042658716681754No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG32560.2010549208438873No Hit
GTTTATTACTGTCTATACCGCAGCACCGGGCCTCCGTGGACGTTCGGCCA31730.19592974933588894No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30860.19055758161063763No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30140.18611164970008484No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA29990.18518541388538634No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29820.18413567996206137No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28380.1752438161409558No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28350.1750585689780161No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28210.17419408221763083No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCTATACCGCAGCACC27470.16962465219845158No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC27320.16869841638375307No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26670.16468472785339294No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26170.16159727513773126No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25280.1561016093038535No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGGCGTCA24740.1527671603709389No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24650.15221141888211984No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCTATACCGCAGCACCGGG24280.14992670387253018No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCGTGGTCCCTTGGCCGAACGTC24210.14949446049233756No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24140.14906221711214493No Hit
GTCAGAGTGTTGGCGGCTACATGGCCTGGTATCAACAAAAGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGATG23930.14776548697156702No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCGTGGTCC23280.14375179844120686No Hit
CCGCAGCACCGGGCCTCCGTGGACGTTCGGCCAAGGGACCACGGTGGACATCAAACGAACTGTGGCTGCACCAT22830.14097309099711136No Hit
CATCTATGATGCATCGAACAGGGCCTCTGACGCCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA22320.13782388922713648No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22250.13739164584694386No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21950.13553917421754685No Hit
TCATAGATGAGGAGCCTGGGAGGCTGGCCAGGCTTTTGTTGATACCAGGC21930.13541567610892039No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21790.13455118934853513No Hit
GGTATAGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACC21660.1337484516424631No Hit
CTGCTACTCTGGCTCCCAGTATCAGATACCACCGGAGAAATTGTGTTGCG21570.133192710153644No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG21420.13226647433894548No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21190.13084624608974113No Hit
GTGCTGCGGTATAGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC20940.12930251973191031No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20920.12917902162328385No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20830.12862328013446472No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20700.12782054242839272No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20680.12769704431976625No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT20470.12640031417918834No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT20390.12590632174468247No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20360.12572107458174278No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19990.12343635957215315No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGG19940.12312761430058698No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19790.12220137848588848No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATCCAATACCTAC19770.12207788037726201No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19640.12127514267119No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19610.12108989550825028No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19440.12004016158492534No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18640.11510023723986668No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18530.1144209976424211No Hit
ATAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA18520.11435924858810788No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGATGGG18260.11275377317596381No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18220.11250677695871089No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCGG18190.11232152979577117No Hit
GAACAGGGCCTCTGACGCCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG17750.10960457140598892No Hit
GCGGTATAGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGA17620.10880183369991689No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17510.10812259410247131No Hit
CTATACCGCAGCACCGGGCCTCCGTGGACGTTCGGCCAAGGGACCACGGTGGACATCAAACGAACTGTGGCTGCA17320.1069493620705199No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17220.10633187152738757No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17180.10608487531013464No Hit
CAATAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA17140.1058378790928817No Hit
GTATCAGATACCACCGGAGAAATTGTGTTGCGGCAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC17040.10522038854974936No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16960.10472639611524348No Hit
GTATTGGATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG16940.10460289800661703No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16850.10404715651779793No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCC16830.10392365840917146No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16660.1028739244858465No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16590.10244168110565388No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16570.10231818299702741No Hit
CTCCCAGGCTCCTCATCTATGATGCATCGAACAGGGCCTCTGACGCCCCA16570.10231818299702741No Hit
ACAAAAGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCGAACAGGGCCTCTGACGCCCCAGCCAGGTT16520.10200943772546124No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16440.10151544529095537No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16420.10139194718232891No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16330.1008362056935098No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16210.100095217041751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATA150.00247431968.1504934
GCGCTAA200.005845784754.8523763
TTCGATC200.005845784754.8523763
CGTATGC3600.046.96526741
CTTATAC3750.046.8468631
ATCTCGT3400.046.30079337
TCGTATG3650.046.2344240
GGCAATC3500.045.70551733
ATGCCGT3750.045.35700644
AATCTCG3500.044.8922636
TATACAC3950.044.474873
GTATGCC3950.043.77673342
TACACAT4100.042.847745
TCTCCGA3950.042.76297811
ACGAGAC4100.042.04971322
GCAATCT3800.041.24897834
CCCACGA4200.041.03838319
CGAGACA4300.040.89270423
CACGAGA4250.040.55933821
CTTGAAA4200.040.15001357
Produced by FastQC (version 0.11.5)