FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619458
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65200.40260383412228046No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46980.29009705716356954No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC46490.2870713535022211No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44450.2744745464223215No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41880.25860503946382063No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40280.24872519077370328No Hit
GTCTATACCGCAGCACCGGGCCTCCGTGGACGTTCGGCCAAGGGACCACGGTGGACATCAAACGAACTGTGGCTG39050.24113005709317561No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38030.23483165355322583No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCTATACCG37810.23347317435833467No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCGGCTACATGGCCTGGTATCAACAAAAGCCTGGCCAGCCTCCCAGGCT35810.22112336349568804No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35360.21834465605159256No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGGCGTCAGAGGCCCTGTT34550.21334298265222065No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33110.2044511188311151No Hit
GTATCAGATACCACCGGAGAAATTGTGTTGCGGCAGTCTCCAGCCACCCT32400.20006693597487557No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA32290.19938769637742998No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30810.19024883633907147No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30800.19018708728475825No Hit
GTTTATTACTGTCTATACCGCAGCACCGGGCCTCCGTGGACGTTCGGCCA30420.18784062322085537No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29280.1808012310291468No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29250.18061598386620709No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28150.17382358789175142No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27660.170797884230403No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27460.16956290314413835No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCTATACCGCAGCACC27050.1670311919172958No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26610.16431423352751354No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26270.1622147656808636No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26140.16141202797479157No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26090.1611032827032254No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25950.16023879594284013No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25890.15986830161696075No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25850.1596213053997078No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24810.15319940375113156No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCTATACCGCAGCACCGGG24000.14819773035175968No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGGCGTCA23880.14745674170000087No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCGTGGTCCCTTGGCCGAACGTC23700.14634525872236265No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23460.14486328141884505No Hit
GTCAGAGTGTTGGCGGCTACATGGCCTGGTATCAACAAAAGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGATG23250.14356655127826717No Hit
CTGCTACTCTGGCTCCCAGTATCAGATACCACCGGAGAAATTGTGTTGCG22930.1415905815402437No Hit
GTGCTGCGGTATAGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC22450.1386266269332085No Hit
CATCTATGATGCATCGAACAGGGCCTCTGACGCCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21940.13547742516323363No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21530.13294571393639107No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21480.13263696866482488No Hit
CCGCAGCACCGGGCCTCCGTGGACGTTCGGCCAAGGGACCACGGTGGACATCAAACGAACTGTGGCTGCACCAT21380.13201947812169257No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21200.13090799514405435No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21100.13029050460092204No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATCCAATACCTAC20950.12936426878622354No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20840.12868502918877797No Hit
GTATCAGATACCACCGGAGAAATTGTGTTGCGGCAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC20750.12812928769995888No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCGTGGTCC20710.12788229148270594No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT20600.12720305188526038No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20590.12714130283094713No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20340.1255975764731163No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19580.1209046483453106No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19490.1203489068564915No Hit
GGTATAGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACC19180.11843468617278126No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18980.1171997050865166No Hit
GCGGTATAGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGA18970.11713795603220337No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18930.11689095981495044No Hit
TCATAGATGAGGAGCCTGGGAGGCTGGCCAGGCTTTTGTTGATACCAGGC18610.11491499007692696No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18170.1121980316871447No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18150.11207453357851825No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18040.11139529398107267No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17840.11016031289480802No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17800.10991331667755508No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCGG17770.10972806951461539No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGG17690.10923407708010953No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17600.10867833559129042No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17530.10824609221109778No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG17140.1058378790928817No Hit
GAACAGGGCCTCTGACGCCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG17050.10528213760406259No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC16850.10404715651779793No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16700.10312092070309943No Hit
ATAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA16690.10305917164878621No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16590.10244168110565388No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC16550.10219468488840094No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16490.10182419056252152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTT252.296337E-456.4611551
CTTGCGT252.3232294E-456.32827450
AATGGTA4950.042.4621270
GTCCTAA6200.035.03124670
GACAGGT2250.032.977961
ATTCACT2150.029.79832
ATCAACG25200.028.9021113
TCAACGC25150.028.8228264
CAACGCA25300.028.6545965
TAATCGT605.6019606E-428.59101938
TTAATCG605.6640315E-428.53722437
AACGCAG25550.028.37866
TTCGTAC500.00852168727.05801826
ACGCAGA27050.026.9395837
GTCGTAA657.9519337E-426.92810848
AGAGTAC27500.026.51677711
CGCAGAG27600.026.4100888
GCAGAGT28300.025.7624079
TGTTAGA16900.025.70339870
TCACTTT2550.025.121734
Produced by FastQC (version 0.11.5)