FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569993
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT51710.9072041235594122No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20190.35421487632304255No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18820.3301794934323755No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14360.25193291847443744No Hit
CATCTATGCTGCGTCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA13590.23842398064537634No Hit
GTACTATAACTCACCTCGTACGTTCGGGCAGGGGACCAAGGTCGAAGTCCGACGAACTGTGGCTGCACCAT13080.22947650234301123No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12600.22105534629372642No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12410.21772197202421786No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12390.2173710905221643No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12240.21473947925676282No Hit
GTCTGGGACAGACTTCACTCTCAGTATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTTCTGTCAGCAGTA11640.2042130341951568No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11030.193511148382524No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11000.19298482612944368No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10430.18298470332091796No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10260.18000221055346294No Hit
GTTATAGTACTGCTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATACTGAGAGTGAAGT10030.1759670732798473No Hit
GCCATATACTACTGTGGGAGAGATGGCGAGGGATATAGTGGCTACGGGTGGATCGACCCCTGGGGCCAGGGAACC9840.17263369901033873No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9680.16982664699391045No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9520.16701959497748217No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC9040.15859843892819736No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8660.15193169038918022No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8660.15193169038918022No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8530.14965096062583225No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGCT8320.14596670485427013No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8320.14596670485427013No Hit
GCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTCTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA8230.14438773809502925No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8090.1419315675806545No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8050.14122980457654744No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7950.13947539706627976No Hit
GTATTTCTGTCAGCAGTACTATAACTCACCTCGTACGTTCGGGCAGGGGA7910.1387736340621727No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7710.13526481904163737No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7700.13508937829061057No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7540.13228232627418232No Hit
GAGTTATAGTACTGCTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATACTGAGAGTGAAGT7330.1285980705026202No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGCTCCTCGGTGAAGGTCTCCTGCG7260.12736998524543283No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC7110.12473837398003133No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6850.12017691445333539No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6790.11912426994717477No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6770.11877338844512127No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6680.11719442168588036No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6490.11386104741637178No Hit
GTGCAGCCACAGTTCGTCGGACTTCGACCTTGGTCCCCTGCCCGAACGTA6350.11140487690199705No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6340.11122943615097028No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6330.1110539953999435No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6320.11087855464891674No Hit
GTATATGGCCGTGTCCTCAGATGTCAGGCTCCGCAGCTCCATGTAAGATG6290.11035223239583644No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6190.10859782488556877No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6140.10772062113043493No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6110.10719429887735463No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6070.10649253587324756No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5960.10456268761195313No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5900.10351004310579252No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5860.10280828010168547No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC5840.10245739859963193No Hit
ATATACTACTGTGGGAGAGATGGCGAGGGATATAGTGGCTACGGGTGGAT5790.1015801948444981No Hit
CCCTATCTTTGTCTCCAGGGGACAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAG5780.10140475409347131No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5780.10140475409347131No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5760.10105387259141778No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5750.10087843184039103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGC308.946256E-657.68651641
TATCGTA252.6728405E-454.74603738
CTAATCG252.833467E-454.1030734
ATCGCGT200.007710809451.13884437
AATCGCG200.007799367450.9913536
TAATCGC200.00789428750.83556435
CCGTCTT7600.050.3260847
GAGTAAG200.00866987249.6445271
CGTCTTC7600.049.57098848
ATGCCGT7700.049.5508444
TCTACGT250.00883606649.40500368
CTTATAC8100.049.0316281
CGTATGC7800.048.81167241
TCGTATG7700.048.7990440
GCCGTCT7700.048.5747546
CTTGAAA8350.048.0715157
TTATACA8350.047.959982
GTATGCC8050.047.9236542
TATACAC8300.047.447233
CGAGACT8250.047.38354523
Produced by FastQC (version 0.11.5)