FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569993
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92841.6287919325325049No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18020.3161442333502341No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16940.2971966322393433No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14580.25579261499702627No Hit
GTACTATAACTCACCTCGTACGTTCGGGCAGGGGACCAAGGTCGAAGTCCGACGAACTGTGGCTGCACCAT14070.24684513669466115No Hit
CATCTATGCTGCGTCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA12710.22298519455502086No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12350.21666932751805723No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12210.2142131570036825No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12080.2119324272403345No Hit
GTCTGGGACAGACTTCACTCTCAGTATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTTCTGTCAGCAGTA11740.20596744170542444No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10370.18193205881475738No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10020.17579163252882052No Hit
GTTATAGTACTGCTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATACTGAGAGTGAAGT9850.17280913976136547No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA9650.16930032474083015No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9380.16456342446310745No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9360.16421254296105392No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9220.16175637244667918No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9000.1578966759240903No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8870.1556159461607423No Hit
GCCATATACTACTGTGGGAGAGATGGCGAGGGATATAGTGGCTACGGGTGGATCGACCCCTGGGGCCAGGGAACC8620.15122992738507315No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8520.14947551987480548No Hit
GTATTTCTGTCAGCAGTACTATAACTCACCTCGTACGTTCGGGCAGGGGA8500.14912463837275194No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGCTCCTCGGTGAAGGTCTCCTGCG7930.13912451556422623No Hit
GAGTTATAGTACTGCTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATACTGAGAGTGAAGT7810.13701922655190502No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7760.13614202279677118No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7680.13473849678855707No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7630.13386129303342323No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7620.13368585228239643No Hit
GCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTCTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA7440.13052791876391465No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7430.13035247801288788No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7400.12982615575980758No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC7320.12842262975159344No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7070.12403661097592426No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGCT7050.12368572947387073No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6930.12158044046154952No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6900.12105411820846923No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6790.11912426994717477No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6570.1152645734245859No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6560.11508913267355915No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6460.11333472516329149No Hit
GTGTATTTCTGTCAGCAGTACTATAACTCACCTCGTACGTTCGGGCAGGGGACCAAGGTCGAAGTCCGACGAA6460.11333472516329149No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6370.11175575840405058No Hit
GTGCAGCCACAGTTCGTCGGACTTCGACCTTGGTCCCCTGCCCGAACGTA6340.11122943615097028No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6280.11017679164480967No Hit
GTATATGGCCGTGTCCTCAGATGTCAGGCTCCGCAGCTCCATGTAAGATG6160.10807150263248846No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6150.10789606188146171No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6030.1057907728691405No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5930.10403636535887284No Hit
GTCTCCAGGCACCCTATCTTTGTCTCCAGGGGACAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG5920.10386092460784606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)