FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223333
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGAAACA59450.4859674348685109No Hit
GTATAATAACTGGCCTCTGTACACTTTTGGCCAGGGGACCAAGCTGGATATCAGACGAACTGTGGCTGCACCAT45020.368010999458038No Hit
GTGTATTACTGTGCGAGAGAGCCCTATTGTGGTGGTGACTGCTATCCCCT44390.362861134294587No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38970.31855594511061175No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35930.29370580209967356No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT34040.2782562066093206No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG30880.25242513690058227No Hit
CTTCTATGGTGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA30090.24596736947339767No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28420.23231613959567837No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27500.22479570157921025No Hit
CCATAGAAGAGGAGCCGGGGAGCCTGGCCAGGTTTCAGCTGGTACCAGGC25670.20983656943775733No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25540.2087738988484738No Hit
CTCCTGTGCAGCCTCAGGCTTCACCTTCAGTGGCCATAGCATGAACTGGGTCCGCCAGACTCCGGGGAAGGGG24690.20182566807238914No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24130.19724801014932158No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24050.1965940590174548No Hit
CAGTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGAAA23410.19136244996252044No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22850.1867847920394529No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22810.18645781647351947No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGCGGCCCTGGT22660.18523165810126924No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT22500.18392375583753565No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21780.1780381956507345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21760.17787470786776782No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21610.17664854949551756No Hit
CTCCTGCAGGGCCAGTCAGGGTATTTTCAGCAACTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCCGGCT21210.1733787938361836No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20510.16765672143234917No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20400.16675753862603232No Hit
ACCACATACTACGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCGAGAATTCACTGTTTCTG20180.16495917301339863No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19770.1616076734625813No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19680.16087197843923118No Hit
GTATTACTGTGCGAGAGAGCCCTATTGTGGTGGTGACTGCTATCCCCTCGGGTACTTCGATCTCTGGGGCCGTGG19550.15980930784994765No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19240.1572752472139638No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19240.1572752472139638No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG18990.1552316499268801No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18970.1550681621439134No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGGGTATTTTCAG18930.15474118657798No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18290.14950957752304567No Hit
CTCCAGAGACAATGCCGAGAATTCACTGTTTCTGCAAATGAACAGCCTGAGAGACGAGGACACGGCTGTGTATTA18250.14918260195711225No Hit
CTCCAGCCCCTTCCCCGGAGTCTGGCGGACCCAGTTCATGCTATGGCCAC18180.14861039471672882No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18180.14861039471672882No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTGGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT17840.14583110240629493No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17840.14583110240629493No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17770.14525889516591148No Hit
CTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATCAGCACTGAACACAGAGGACTCA17740.14501366349146144No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17590.1437875051192112No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC17450.14264309063844433No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17400.14223437118102755No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17320.14158042004916077No Hit
CTTTTGGCCAGGGGACCAAGCTGGATATCAGACGAACTGTGGCTGCACCA17310.14149867615767744No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17270.14117170059174403No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17080.1396185666535604No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16850.13773845714944338No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16670.13626706710274308No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16180.13226161642005899No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16170.13217987252857563No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16150.13201638474560892No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16050.13119894583077543No Hit
GGGCTGGAGTGGGTGGCATATATTACTGGTTTTTCTAGAACCACATACTA16030.13103545804780875No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15900.1299727874585252No Hit
GTATGTGGTTCTAGAAAAACCAGTAATATATGCCACCCACTCCAGCCCCTTCCCCGGAGTCTGGCGGACCCAGTT15710.12841965352034157No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15650.12792919017144147No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15570.1272752390395747No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA15550.12711175125660795No Hit
GTGCTGGGTTTTCCTTGTGGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC15470.12645780012474117No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15310.1251498978610076No Hit
CTGTACACTTTTGGCCAGGGGACCAAGCTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15200.12425071505469075No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGGGTATTTTCAGCAACTTAGCCTGGTA15180.12408722727172405No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15090.1233515322483739No Hit
CCACAATAGGGCTCTCTCGCACAGTAATACACAGCCGTGTCCTCGTCTCT14670.11991828880607323No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14420.11787469151898951No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCTGTACACTTTTGGCCA14380.11754771595305612No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14280.11673027703822261No Hit
CACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG14250.11648504536377258No Hit
GGCCAAAAGTGTACAGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTG14190.11599458201487249No Hit
GTGCAGCCACAGTTCGTCTGATATCCAGCTTGGTCCCCTGGCCAAAAGTGTACAGAGGCCAGTTATTATACTG14110.11534063088300568No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA13880.11346052137888867No Hit
CCTTGGGGCTGGTCGGGGATGCCGAGGGGACAGTGACCAGGGTGCCACGG13800.11280657024702187No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG13530.11059948517697143No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13140.10741147340912081No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCAGCTTGGTCC13130.10732972951763746No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG13120.10724798562615412No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13110.10716624173467076No Hit
CTATTGTGGTGGTGACTGCTATCCCCTCGGGTACTTCGATCTCTGGGGCC13060.10675752227725403No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12860.10512264444758704No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12790.1045504372072036No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12710.1038964860753368No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGATATCAGACGAACTGTGGCTG12590.10291555937753663No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT12580.10283381548605325No Hit
CTGCTATCCCCTCGGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCCCCTCGGCATCCCCGACCAG12550.10258858381160321No Hit
CAATAGGGCTCTCTCGCACAGTAATACACAGCCGTGTCCTCGTCTCTCAG12340.10087196209045289No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12290.10046324263303613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT950.057.4186832
TCGTATG800.056.02001640
TAAGGCG1100.052.52824829
CGAGACT1250.051.51727323
ACGAGAC1300.049.5236922
TCTGGAG17050.048.95216470
GGTATCA6950.047.7680471
CGTATGC950.047.23566441
GAGACTA1400.043.58733724
CACGAGA1400.043.56416721
GCGAATC1300.041.99781433
ATCTCCG1300.041.68881610
GACTAAG1550.041.5887126
AATCTCG1250.041.088336
AGACTAA1500.040.70316725
CTTATAC1500.040.651581
ATGCCGT1050.039.62286444
GCCGTCT801.5115802E-939.1396746
AGGCGAA1500.038.6014631
GTATCAA16450.037.4801831
Produced by FastQC (version 0.11.5)