FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223333
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGAAACA54170.44280666016530246No Hit
GTGTATTACTGTGCGAGAGAGCCCTATTGTGGTGGTGACTGCTATCCCCT43560.356076391301469No Hit
GTATAATAACTGGCCTCTGTACACTTTTGGCCAGGGGACCAAGCTGGATATCAGACGAACTGTGGCTGCACCAT43150.3527248917506517No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38800.3171662989553948No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT31330.25610361201733295No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29880.24425074775224734No Hit
CTTCTATGGTGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA29170.23844693145692958No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28890.2361581024953958No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27460.22446872601327686No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26370.2155586418415918No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26150.2137602762289581No Hit
CCATAGAAGAGGAGCCGGGGAGCCTGGCCAGGTTTCAGCTGGTACCAGGC24190.19773847349822166No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23430.19152593774548712No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23420.19144419385400377No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT22660.18523165810126924No Hit
CTCCTGTGCAGCCTCAGGCTTCACCTTCAGTGGCCATAGCATGAACTGGGTCCGCCAGACTCCGGGGAAGGGG22510.184005499729019No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22030.18008179293781823No Hit
CTCCTGCAGGGCCAGTCAGGGTATTTTCAGCAACTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCCGGCT21300.17411448885953376No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20900.17084473320019977No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGCGGCCCTGGT20840.1703542698512997No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20580.16822892867273262No Hit
CTCCAGCCCCTTCCCCGGAGTCTGGCGGACCCAGTTCATGCTATGGCCAC20530.16782020921531585No Hit
CAGTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGAAA20400.16675753862603232No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20110.16438696577301518No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19720.1611989540051646No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19510.15948233228401423No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG19360.158256173911764No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19190.15686652775654708No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19170.15670303997358037No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19060.15580385716726353No Hit
ACCACATACTACGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCGAGAATTCACTGTTTCTG18750.1532697965312797No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGGGTATTTTCAG18520.15138968702716268No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18110.14803818747634534No Hit
GTATTACTGTGCGAGAGAGCCCTATTGTGGTGGTGACTGCTATCCCCTCGGGTACTTCGATCTCTGGGGCCGTGG17890.14623982186371168No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17860.14599459018926164No Hit
CTCCAGAGACAATGCCGAGAATTCACTGTTTCTGCAAATGAACAGCCTGAGAGACGAGGACACGGCTGTGTATTA17830.14574935851481158No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17770.14525889516591148No Hit
CTTTTGGCCAGGGGACCAAGCTGGATATCAGACGAACTGTGGCTGCACCA17500.14305181009586107No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17440.14256134674696097No Hit
GGGCTGGAGTGGGTGGCATATATTACTGGTTTTTCTAGAACCACATACTA17380.14207088339806087No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17350.14182565172361083No Hit
CTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATCAGCACTGAACACAGAGGACTCA17290.14133518837471074No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17070.13953682276207705No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG16990.13888287163021026No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16930.13839240828131016No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTGGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT16770.13708450601757657No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16700.13651229877719312No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16390.1339782381412093No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16200.13242510420302567No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16160.13209812863709228No Hit
GTATGTGGTTCTAGAAAAACCAGTAATATATGCCACCCACTCCAGCCCCTTCCCCGGAGTCTGGCGGACCCAGTT15890.12989104356704184No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGGGTATTTTCAGCAACTTAGCCTGGTA15730.12858314130330825No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15630.12776570238847476No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15560.1271934951480913No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15550.12711175125660795No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15430.12613082455880778No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC15050.12302455668244051No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA14910.12188014220167363No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14830.12122619106980682No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14800.12098095939535677No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14770.12073572772090674No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14710.12024526437200662No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14570.11910084989123974No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC14510.11861038654233966No Hit
CTGTACACTTTTGGCCAGGGGACCAAGCTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14470.11828341097640627No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14350.11730248427860607No Hit
GTGCTGGGTTTTCCTTGTGGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC14340.11722074038712273No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14140.11558586255745573No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14030.1146866797511389No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13880.11346052137888867No Hit
CCTTGGGGCTGGTCGGGGATGCCGAGGGGACAGTGACCAGGGTGCCACGG13740.11231610689812178No Hit
CTGCTATCCCCTCGGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCCCCTCGGCATCCCCGACCAG13670.11174389965773832No Hit
GTGCAGCCACAGTTCGTCTGATATCCAGCTTGGTCCCCTGGCCAAAAGTGTACAGAGGCCAGTTATTATACTG13650.11158041187477163No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13550.11076297295993813No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13450.10994553404510465No Hit
GGCCAAAAGTGTACAGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTG13420.10970030237065459No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC13230.10814716843247096No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCTGTACACTTTTGGCCA13030.10651229060280398No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT12960.10594008336242053No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12950.10585833947093719No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA12910.1055313639050038No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12840.10495915666462034No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12670.10356951050940341No Hit
CACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG12650.10340602272643672No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGATATCAGACGAACTGTGGCTG12580.10283381548605325No Hit
CCACAATAGGGCTCTCTCGCACAGTAATACACAGCCGTGTCCTCGTCTCT12500.10217986435418647No Hit
GCCCTATTGTGGTGGTGACTGCTATCCCCTCGGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCCC12430.10160765711380301No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCAGCTTGGTCC12250.10013626706710274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG17700.054.3481570
TGCGGGT306.951543E-445.10714313
GGTATCA5750.041.1460461
GTATCAA15050.038.6269041
CTCTGGA17300.037.0070869
GGCCGTG20500.035.5387870
TATCAAC17550.032.9211432
ATTGTCT16650.032.8482164
CATTGTC16700.032.67490463
TTGTCTC16900.032.280465
TGTCTCT17400.031.65286666
GGCATTG17150.031.63929461
CGGCATT17350.031.3691260
GCATTGT17350.031.34729262
ATCAACG18250.031.285583
TCAACGC18500.031.0428854
AACGCAG18850.030.8362666
CAACGCA18950.030.6647645
ATTCTCG17550.030.13695755
CCGTTAA450.005086859730.0726616
Produced by FastQC (version 0.11.5)