FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900605
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34030.37785710716684895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGCA4850.034.5048170
CTAACGC601.4842088E-533.9970553
GTATCAA25400.032.41971
GGTATCA12600.030.516541
CGAATTA953.6469828E-728.62433415
TAACGCC605.909558E-428.329314
TTCGTAA500.00824566127.24000225
CCTAACG659.3968597E-426.1675662
ATCAACG30950.026.0332873
TCAACGC30900.025.963954
TATCAAC31100.025.9235612
AACGCAG31000.025.8758816
CAACGCA31150.025.749855
AGAGTAC32800.024.24861111
ACGCAGA33300.023.986597
CAGAGTA33750.023.66676910
ATAGCGG1102.3314353E-522.93355463
CGCAGAG34700.022.9208778
CCGAATT1202.7964925E-622.6596714
GGTAGTA4400.022.4822453
Produced by FastQC (version 0.11.5)