FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1184805
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCATAGTTACCCCCTTACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCAT57650.486577960086259No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48640.4105316908689615No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48000.40512995809436997No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45180.3813285730563257No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43950.3709471178801575No Hit
CTATGATACTGTTGGCAGTAATAAGTTGCAGAATCCTCAGGCTGTAGGCTGCTGATGGCGAGAGTGAAATCTGTC43130.364026147762712No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42320.35718957971986953No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37690.3181114191786834No Hit
AGTCAGGACATTGCCCATTATTTAGGCTGGTTTCTGCAGAAACCAGGGAA34160.28831748684382663No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33480.2825781457708231No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT32440.2738003300121117No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31510.2659509370740333No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29040.24510362464709384No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26930.22729478690586216No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26030.2196986001915927No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25390.2142968674170011No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24050.20298698942019994No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23410.19758525664560836No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22270.18796342014086706No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22240.18771021391705808No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22120.18669738902182215No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21490.18138005832183357No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20470.17277104671232818No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20050.16922615957900244No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19650.16585007659488268No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19200.16205198323774797No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18830.15892910647743722No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18520.15631264216474441No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18480.15597503386633244No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG18450.15572182764252346No Hit
GATCTATGATGCATCCAGTTTGCAGCGTGGCGTCCCATCAAGGATCAGCGGCAGTGGATCTGGGACAGATTTCA18140.15310536332983066No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17890.1509953114647558No Hit
GCCTACAGCCTGAGGATTCTGCAACTTATTACTGCCAACAGTATCATAGTTACCCCCTTACTTTCGGCGGAGGGA17880.1509109093901528No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17730.1496448782711079No Hit
GACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT17350.14643759943619414No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17210.14525597039175223No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC17200.14517156831714922No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17010.14356792889969236No Hit
GTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGTA16830.14204869155683847No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16690.14086706251239656No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16610.1401918459155726No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16400.13841940234890973No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16240.13706896915526184No Hit
GTCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGTGAGGATGGAGACTGGGTCAT16200.13673136085684987No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16190.13664695878224686No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15710.13259565920130315No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15320.12930397829178641No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15210.12837555547115348No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14740.12440865796481278No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14570.12297382269656187No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCT14550.12280501854735588No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14480.12221420402513494No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14260.12035735838386909No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14130.11926013141403016No Hit
GGCTGTAGGCTGCTGATGGCGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGATCCTTGATGGGACGCCACG13960.11782529614577926No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13920.1174876878473673No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13910.11740328577276431No Hit
ACCTTGGTCCCTCCGCCGAAAGTAAGGGGGTAACTATGATACTGTTGGCAGTAATAAGTTGCAGAATCCTCAGG13660.11529323390768946No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGACATTGCCCATTATTTAGGCTGGTTTCTGCAGAAA13380.11292997581880562No Hit
GTCTCCATCCTCACTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT13370.11284557374420262No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA13020.10989150113309784No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12840.10837226379024398No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12820.10820345964103797No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12810.10811905756643499No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12780.107865851342626No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12730.10744384096961104No Hit
GATTTCACTCTCGCCATCAGCAGCCTACAGCCTGAGGATTCTGCAACTTATTACTGCCAACAGTATCATAGTTAC12710.10727503682040505No Hit
CAGTATCATAGTTACCCCCTTACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCAT12690.10710623267119906No Hit
ACTTATTACTGCCAACAGTATCATAGTTACCCCCTTACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAA12580.10617780985056613No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12470.1052493870299332No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG12310.1038989538362853No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12180.10280172686644637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.041.740491
GTGGTAT5700.041.3679771
TGGTATC6250.037.188372
GTATCAA43750.037.120311
TATCAAC50750.032.1243672
ATCAACG51950.031.3170853
TCAACGC52300.031.2344764
TATGATG18650.030.90120770
TAACCAT500.004485981530.855166
AACGCAG53750.030.6480126
CAACGCA53450.030.5624565
CGGGATA554.034462E-430.23896617
CGCAGAG57150.028.7762898
ACGCAGA58150.028.2790367
AGAGTAC58400.028.17106211
CAGAGTA58800.027.97705810
GCAGAGT59450.027.6688379
TAGGCAA2105.8691694E-727.1044170
GTAATAC3750.026.568731
TCTGGAG4900.026.13639570
Produced by FastQC (version 0.11.5)