FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285626
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC59140.4600093650875138No Hit
TCACTACCCCGTGGACGTTCGGCCAAGGGACCAAAGTAGAAATGAAACGA56480.43931905546403077No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55530.43192965916992965No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44060.34271242180851974No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41680.32420003951382437No Hit
GCCCCTAACCTCTTGATCTTTGGTGGATCCTCTTTGGAAAATGGAGTCCC40290.3133881859887713No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38990.30327638053368555No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36550.28429729952567856No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33170.25800660534245573No Hit
GGGTAGTGAAACTCTCTTGACAATAGTAAGTTGCAAAATCTTCAGGTTGC32990.2566065092025208No Hit
GTCTGGGGCTGAGGTGAAGAAACCTGGGGCCTCAGTGAAGGTCTCCTGCA32240.2507727752861252No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31500.24501682448861486No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30710.23887195809667822No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29290.2278267552149692No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28520.22183745506080305No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28510.22175967194191779No Hit
ATATGGAACTGACCAGCCTGACATCTGAAGACACGGCCATTTATTATTGTGTGAAACTGACCGACGGTACTTAC27900.21701490168991605No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27540.21421470941004614No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27120.2109478184168646No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26730.20791427678033889No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26080.20285837405279605No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26010.20231389222059915No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25860.20114714543732004No Hit
GTGCAGCCACAGTTCGTTTCATTTCTACTTTGGTCCCTTGGCCGAACGTC25070.19500227904538334No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC24240.18854628017790556No Hit
GTCCTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAAGAGAGTTTC23770.18489047359029764No Hit
GTTCCATATAGGATGTGTCTGTAGATGTGTCGTCGGTCATGGTGACTCTG23180.18030126957606646No Hit
GTACTTACCTCAGTCGCTTTGACTTCTGGGGCCAGGGAACCCGGGTCACCGTCTCCTCAGCATCCCCGACCAGCC23170.18022348645718117No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23050.17929008903055788No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG22480.17485645125409724No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT22020.1712784277853746No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21690.16871158486216054No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21300.1656780432256348No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG21000.1633445496590766No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC20780.16163332104360054No Hit
CACCATCAGTCCTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAAGAGAGTTTCACTACCCCGTGGACGTT20360.158366430050419No Hit
GGGTAAGATCATCTACGATCCGAAGTTGCAGGGCAGAGTCACCATGACCG20310.15797751445599265No Hit
GGTTAGGGGCTGTCCCTGGTTTTTGCTGATACCAATTTAAATAATTCATA20250.157510815742681No Hit
AGCTGGTACAGTCTGGGGCTGAGGTGAAGAAACCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTTTCCGGATCCA20200.15712190014825464No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20160.1568107676727135No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20150.15673298455382825No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20090.1562662858405166No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCTACTTTGGTCC19880.15463284034392583No Hit
GGTAAGTACCGTCGGTCAGTTTCACACAATAATAAATGGCCGTGTCTTCA19830.15424392474949947No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19540.1519882143018265No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18780.14607669726654562No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC18710.14553221543434872No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCCGCTGACCCAGTC18630.14490995048326652No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18600.14467660112661068No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTGGGAGACAGAGTCACCATCACCT18380.14296537251113464No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18080.1406318789445764No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18030.14024296335015005No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17970.1397762646368384No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17850.1388428672102151No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17800.13845395161578872No Hit
CCATCAGTCCTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAAGAG17770.1382206022591329No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17530.13635380740588632No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17490.13604267493034522No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17320.13472036190929554No Hit
ATCTTACCCTCTTCAGGATGAAAGCCTCCCATCCACTCAAGCCCTTTTCC17200.13378696448267227No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17010.13230908522385204No Hit
GGACTGATGGTGAGAGTGAAATTTGTCCCATATCCACTGCCACTAAATCT16880.13129790467834346No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16750.13028672413283487No Hit
GCAGAGGACTGATGGTGAGAGTGAAATTTGTCCCATATCCACTGCCACTA16670.1296644591817527No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16640.12943110982509687No Hit
GTGGGAGACAGAGTCACCATCACCTGCCGGACAAGTCAGAACATTATGAATTATTTAAATTGGTATCAGCAAAAA16490.12826436304181776No Hit
AGTCAGAACATTATGAATTATTTAAATTGGTATCAGCAAAAACCAGGGAC16260.12647535130745644No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA16210.12608643571303008No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA16010.12453077333532457No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC16000.1244529902164393No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15930.12390850838424239No Hit
CTCACCATCAGTCCTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCA15920.12383072526535711No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15860.12336402655204547No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15850.12328624343316019No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15820.12305289407650435No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCTCCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG15660.12180836417433998No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15510.12064161739106084No Hit
GTTCTGACTTGTCCGGCAGGTGATGGTGACTCTGTCTCCCACAGAGGCAGACAGGGAGGATGGAGACTGGGTCAG15340.11931930437001119No Hit
GGATATGGGACAAATTTCACTCTCACCATCAGTCCTCTGCAACCTGAAGA15310.11908595501335535No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGGTTCCCTGG15130.11768585887342042No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15050.11706359392233821No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14770.11488566659355053No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14700.11434118476135362No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14690.11426340164246834No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14660.11403005228581252No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14590.1134855704536156No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14480.11262995614587758No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14430.1122410405514512No Hit
ATCTTAATGGGACTCCATTTTCCAAAGAGGATCCACCAAAGATCAAGAGGTTAGGGGCTGTCCCTGGTTTTTG14400.11200769119479538No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14380.11185212495702483No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14320.11138542624371317No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14160.11014089634154879No Hit
CATTAAGATTTAGTGGCAGTGGATATGGGACAAATTTCACTCTCACCATCAGTCCTCTGCAACCTGAAGATTTTG14150.11006311322266352No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14120.10982976386600769No Hit
TCGCATGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGA14090.10959641450935187No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13820.10749627029944944No Hit
GTCTCCCACAGAGGCAGACAGGGAGGATGGAGACTGGGTCAGCGGGATGT13690.10648508975394089No Hit
CCCCTAACCTCTTGATCTTTGGTGGATCCTCTTTGGAAAATGGAGTCCCA13670.10632952351617034No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13620.10594060792174397No Hit
ACCAGGGACAGCCCCTAACCTCTTGATCTTTGGTGGATCCTCTTTGGAAAATGGAGTCCCATTAAGATTTAGTGG13590.10570725856508814No Hit
GTTAGGGGCTGTCCCTGGTTTTTGCTGATACCAATTTAAATAATTCATAA13450.1046182949006943No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC13360.10391824683072683No Hit
TCTACAGACACATCCTATATGGAACTGACCAGCCTGACATCTGAAGACAC13350.10384046371184155No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13290.10337376499852989No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG13280.10329598187964462No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT12990.10104027143197165No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT12960.10080692207531584No Hit
GCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGT12870.10010687400534837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)