FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285626
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54430.4233735160925495No Hit
TCACTACCCCGTGGACGTTCGGCCAAGGGACCAAAGTAGAAATGAAACGA50470.39257140101398075No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48330.375925813572532No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44650.3473016258227509No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42290.3289448097658262No Hit
GCCCCTAACCTCTTGATCTTTGGTGGATCCTCTTTGGAAAATGGAGTCCC40730.3168106432197233No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35420.2755078070916425No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33570.2611179300978667No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33480.2604178820278993No Hit
GGGTAGTGAAACTCTCTTGACAATAGTAAGTTGCAAAATCTTCAGGTTGC33170.25800660534245573No Hit
GTCTGGGGCTGAGGTGAAGAAACCTGGGGCCTCAGTGAAGGTCTCCTGCA32740.25466193123038894No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31450.2446279088941885No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28850.22440429798401712No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28400.22090405763417978No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28220.21950396149424484No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27410.21320352886453758No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26480.20596969880820704No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC25860.20114714543732004No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25680.1997470492973851No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25470.19811360380079432No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25400.1975691219685974No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24740.1924354361221693No Hit
ATATGGAACTGACCAGCCTGACATCTGAAGACACGGCCATTTATTATTGTGTGAAACTGACCGACGGTACTTAC24630.19157982181443126No Hit
GTCCTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAAGAGAGTTTC24050.18706840091908533No Hit
GTTCCATATAGGATGTGTCTGTAGATGTGTCGTCGGTCATGGTGACTCTG24050.18706840091908533No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23550.1831792449748216No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23540.18310146185593634No Hit
GTACTTACCTCAGTCGCTTTGACTTCTGGGGCCAGGGAACCCGGGTCACCGTCTCCTCAGCATCCCCGACCAGCC23430.1822458475481983No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23380.18185693195377192No Hit
GTGCAGCCACAGTTCGTTTCATTTCTACTTTGGTCCCTTGGCCGAACGTC23370.18177914883488666No Hit
GTGGGAGACAGAGTCACCATCACCTGCCGGACAAGTCAGAACATTATGAATTATTTAAATTGGTATCAGCAAAAA22700.17656767986957328No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22690.176489896750688No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT21960.17081172907206296No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCCGCTGACCCAGTC21460.16692257312779923No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21260.16536691075009374No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG20930.16280006782687967No Hit
CACCATCAGTCCTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAAGAGAGTTTCACTACCCCGTGGACGTT20570.15999987554700978No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19770.15377722603618782No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC19530.15191043118294123No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTGGGAGACAGAGTCACCATCACCT19520.15183264806405594No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19410.15097703375631794No Hit
GGGTAAGATCATCTACGATCCGAAGTTGCAGGGCAGAGTCACCATGACCG19340.150432551924121No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19330.15035476880523574No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG19120.14872132330864496No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19010.14786570900090695No Hit
GGTTAGGGGCTGTCCCTGGTTTTTGCTGATACCAATTTAAATAATTCATA18920.1471656609309395No Hit
AGCTGGTACAGTCTGGGGCTGAGGTGAAGAAACCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTTTCCGGATCCA18360.1428098062733641No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18180.14140971013342918No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCTACTTTGGTCC17990.13993183087460895No Hit
ATCTTACCCTCTTCAGGATGAAAGCCTCCCATCCACTCAAGCCCTTTTCC17870.13899843344798565No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17590.13682050611919797No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17250.13417588007709863No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17100.1330091332938195No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAG17090.13293135017493424No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16720.1300533747761791No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16540.12865327863624412No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16500.12834214616070302No Hit
GGACTGATGGTGAGAGTGAAATTTGTCCCATATCCACTGCCACTAAATCT16420.12771988120962086No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC16400.12756431497185028No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16270.12655313442634172No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16250.12639756818857117No Hit
AGTCAGAACATTATGAATTATTTAAATTGGTATCAGCAAAAACCAGGGAC16240.1263197850696859No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16200.12600865259414482No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA16180.12585308635637427No Hit
CCATCAGTCCTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAAGAG16010.12453077333532457No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15380.11963043684555229No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15260.11869703941892898No Hit
CTCACCATCAGTCCTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCA15200.11823034070561735No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15020.11683024456568239No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14990.11659689520902657No Hit
GTTCTGACTTGTCCGGCAGGTGATGGTGACTCTGTCTCCCACAGAGGCAGACAGGGAGGATGGAGACTGGGTCAG14920.11605241337682966No Hit
GCAGAGGACTGATGGTGAGAGTGAAATTTGTCCCATATCCACTGCCACTA14910.11597463025794438No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14850.11550793154463274No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14790.11504123283132107No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14750.11473010035577998No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14750.11473010035577998No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC14720.11449675099912417No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14620.11371891981027142No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14580.11340778733473031No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGGTTCCCTGG14300.11122986000594262No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14120.10982976386600769No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14070.1094408482715813No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14040.10920749891492548No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13990.10881858332049911No Hit
GGTAAGTACCGTCGGTCAGTTTCACACAATAATAAATGGCCGTGTCTTCA13900.10811853525053165No Hit
TCGCATGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGA13820.10749627029944944No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13790.10726292094279362No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA13690.10648508975394089No Hit
GTCTCCCACAGAGGCAGACAGGGAGGATGGAGACTGGGTCAGCGGGATGT13670.10632952351617034No Hit
GGATATGGGACAAATTTCACTCTCACCATCAGTCCTCTGCAACCTGAAGA13620.10594060792174397No Hit
ATCTTAATGGGACTCCATTTTCCAAAGAGGATCCACCAAAGATCAAGAGGTTAGGGGCTGTCCCTGGTTTTTG13540.10531834297066175No Hit
CACCTGCACTGCTGCCCACCCCGAGTTGAAGACCCCACTAACCGCCAACA13510.10508499361400593No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCTCCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG13510.10508499361400593No Hit
CCCCTAACCTCTTGATCTTTGGTGGATCCTCTTTGGAAAATGGAGTCCCA13480.10485164425735011No Hit
CATTAAGATTTAGTGGCAGTGGATATGGGACAAATTTCACTCTCACCATCAGTCCTCTGCAACCTGAAGATTTTG13270.10321819876075934No Hit
GCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGT13260.10314041564187407No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13130.10212923509636551No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13070.10166253638305385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)