FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740629
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30470.4114070607551149No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23660.31945819026800193No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22590.3050110109109959No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20390.275306529990049No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19730.2663951857137649No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGCAGCCTTGAGCCTGAAGATT19640.26518000240336254No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT19090.2577538821731258No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT17050.23020972713733867No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16830.227239279045244No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16330.22048826065411967No Hit
GCGTACTACCTGGCCTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCAT16250.2194080977115398No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT15910.21481740520557527No Hit
GCTTTGTACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGGTTGG15850.21400728299864036No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCT15160.2046908776188888No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14730.19888500180252192No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14550.19645463518171716No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14270.19267406488268757No Hit
GTTTATTACTGTCACCAGCGTACTACCTGGCCTCCGTTCACTTTTGGCCA14270.19267406488268757No Hit
GTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCA13220.17849692626132652No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13140.17741676331874665No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13130.17728174295092414No Hit
GTAGTACGCTGGTGACAGTAATAAACTGCAAAATCTTCAGGCTCAAGGCTGCTGATAGTGAGAGTGAAGTCTGTC13040.17606655964052179No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12950.17485137633011938No Hit
GTGTTAGTAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCC12880.17390623375536202No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGGTACTTAGCCTGGTA12870.1737712133875395No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12780.17255603007713716No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCC12670.1710708060310898No Hit
GTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12640.17066574492762232No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12560.16958558198504242No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12290.1659400320538353No Hit
GTAATAAACTGCAAAATCTTCAGGCTCAAGGCTGCTGATAGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12270.16566999131819035No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12140.16391472653649802No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12040.16256452285827316No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11370.15351815821416662No Hit
GACTTCACTCTCACTATCAGCAGCCTTGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTACTACCTGG10780.14555195651263994No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10740.14501187504135No Hit
GAGTGTTAGTAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCTCCTCATCTATGATGCATC10620.14339163062748014No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10520.14204142694925528No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10260.13853089738587065No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10210.13785579554675823No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10210.13785579554675823No Hit
CACTATCAGCAGCCTTGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTACTACCTGGCCTCCGTTCA10150.13704567333982332No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT10030.13542542892595347No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCA9990.13488534745466355No Hit
GGCCAAAAGTGAACGGAGGCCAGGTAGTACGCTGGTGACAGTAATAAACTGCAAAATCTTCAGGCTCAAGGCTG9940.13421024561555112No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT9850.13299506230514874No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9840.13286004193732626No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9830.13272502156950378No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9740.13150983825910137No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9650.13029465494869902No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCA9530.12867441053482917No Hit
GCATATGCTGCGTCGGTGAAAGGCAGGTTCACCATCTCCAGAGATGATTC9420.12718918648878183No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9280.12529890133926705No Hit
GTACAAAGCAGAGCCACTGAGGGTGAACCCAGAGGCTGCACAGGAGAGTT9150.12354363655757471No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9140.12340861618975223No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8950.120843229201125No Hit
GTCAGAGTGTTAGTAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCTCCTCATCTATGATG8940.12070820883330251No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC8820.11908796441943267No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8780.11854788294814274No Hit
GTACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGGTTGGCCGTA8770.11841286258032024No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC8770.11841286258032024No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8750.11814282184467527No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8660.1169276385342729No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8630.11652257743080544No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA8600.11611751632733798No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8580.115847475591693No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8560.11557743485604804No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8480.11449727191346815No Hit
GCATATGCTGTCGCGTCACTGTCAGCTTTGCTTCTAATACGGCCAACCCACTCCAGCCCTTTCCCGGAAGCCTGG8410.11355212933871074No Hit
CTACTACAGTAATACACGGCCGTGTCCTCGCTTTTCAGGCTGTTCATTTG8350.11274200713177582No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACTATCAGCAG8320.11233694602830838No Hit
CTACCTGGCCTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCATA8240.11125678308572848No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8230.11112176271790601No Hit
ACAGAAACCTGGCCAGTCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT8200.11071670161443853No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8190.11058168124661605No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8110.10950151830403616No Hit
CAGTAATACACGGCCGTGTCCTCGCTTTTCAGGCTGTTCATTTGCAGATA8020.10828633499363378No Hit
GGGCTGGAGTGGGTTGGCCGTATTAGAAGCAAAGCTGACAGTGACGCGAC8000.10801629425798882No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA7990.10788127389016633No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7960.10747621278669886No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCAAGGCTGCTGATAGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7920.10693613131540892No Hit
GCCTTGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTACTACC7850.10599098874065152No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7850.10599098874065152No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7840.10585596837282905No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7670.10356062211984679No Hit
TCTCTGGAGATGGTGAACCTGCCTTTCACCGACGCAGCATATGCTGTCGCGTCACTGTCAGCTTTGCTTCTAATA7660.1034256017520243No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7550.10194037770597696No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7480.10099523513121954No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGG7440.1004551536599296No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7440.1004551536599296No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7440.1004551536599296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)