FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740629
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31170.4208584865026889No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20550.27746685587520875No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGCAGCCTTGAGCCTGAAGATT19070.2574838414374809No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18900.2551884951844986No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18840.25437837297756366No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17890.2415514380344275No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17390.2348004196433032No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16990.22939960493040373No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT16470.22237854580363448No Hit
GCGTACTACCTGGCCTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCAT14900.2011803480555042No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT14820.20010018511292427No Hit
GCTTTGTACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGGTTGG14650.19780483885994202No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT14590.1969947166530071No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCT14030.1894335760549479No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGGTACTTAGCCTGGTA13430.18133235398559874No Hit
GTGTTAGTAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCC13210.17836190589350404No Hit
GTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCA12970.17512141706576437No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12750.1721509689736697No Hit
GTTTATTACTGTCACCAGCGTACTACCTGGCCTCCGTTCACTTTTGGCCA12490.16864043941028506No Hit
GTAGTACGCTGGTGACAGTAATAAACTGCAAAATCTTCAGGCTCAAGGCTGCTGATAGTGAGAGTGAAGTCTGTC12430.16783031720335012No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12330.16648011352512526No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12040.16256452285827316No Hit
GTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11840.15986411550182344No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11760.15878395255924357No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11740.15851391182359859No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11470.15486836189239148No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11380.1536531785819891No Hit
GTAATAAACTGCAAAATCTTCAGGCTCAAGGCTGCTGATAGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11350.15324811747852163No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11300.1525730156394092No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCC11070.149467547179492No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11050.14919750644384705No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10950.1478473027656222No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10930.14757726202997723No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10610.1432566102596577No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10560.14258150842054526No Hit
GACTTCACTCTCACTATCAGCAGCCTTGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTACTACCTGG10410.14055620290320794No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10400.14042118253538546No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10320.13934101959280557No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10010.1351553881903085No Hit
GGCCAAAAGTGAACGGAGGCCAGGTAGTACGCTGGTGACAGTAATAAACTGCAAAATCTTCAGGCTCAAGGCTG9840.13286004193732626No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9820.13259000120168127No Hit
GAGTGTTAGTAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCTCCTCATCTATGATGCATC9720.1312397975234564No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9630.13002461421305403No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC9590.12948453274176408No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9520.1285393901670067No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9490.12813432906353925No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT9420.12718918648878183No Hit
CACTATCAGCAGCCTTGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTACTACCTGGCCTCCGTTCA8810.11895294405161018No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8780.11854788294814274No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCA8760.11827784221249775No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCA8540.11530739412040306No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8530.11517237375258058No Hit
GTCAGAGTGTTAGTAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCTCCTCATCTATGATG8530.11517237375258058No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8520.11503735338475808No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC8520.11503735338475808No Hit
GGGCTGGAGTGGGTTGGCCGTATTAGAAGCAAAGCTGACAGTGACGCGAC8440.11395719044217821No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8360.11287702749959831No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8330.11247196639613086No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8250.11139180345355096No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8130.10977155903968112No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8100.10936649793621368No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8050.10869139609710125No Hit
GTACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGGTTGGCCGTA8020.10828633499363378No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8000.10801629425798882No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7980.10774625352234385No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7970.10761123315452135No Hit
GTACAAAGCAGAGCCACTGAGGGTGAACCCAGAGGCTGCACAGGAGAGTT7790.10518086653371662No Hit
CTACTACAGTAATACACGGCCGTGTCCTCGCTTTTCAGGCTGTTCATTTG7750.10464078506242666No Hit
GCCTTGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTACTACC7710.10410070359113673No Hit
GCATATGCTGCGTCGGTGAAAGGCAGGTTCACCATCTCCAGAGATGATTC7660.1034256017520243No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7650.1032905813842018No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA7630.10302054064855683No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7600.10261547954508937No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA7580.1023454388094444No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7540.10180535733815446No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7430.10032013329210711No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7420.10018511292428463No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGG200.00766207251.22265614
GGTATCA5950.044.2578351
GTATCAA14750.038.488631
AAGCGTC400.002622690334.42737237
TATCAAC16650.034.066592
ATCAACG17500.032.3966063
TCAACGC17650.031.9234684
CAACGCA18000.031.4924475
AACGCAG18000.031.4924476
GACAGGA1351.0913936E-1130.3993191
CGCAGAG19250.030.157068
AACCGGC703.388708E-530.12852156
ACGCAGA19150.029.7795777
GCAGAGT19400.029.7478669
AGAGTAC19600.029.61654511
TAGGCAA1454.425458E-528.96680670
AATGTTG1000.007202362628.00124570
GAGTTTA3100.027.580031
GTATCAC500.00807069727.359391
ACGGGTA500.00812944627.318755
Produced by FastQC (version 0.11.5)