FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493456
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC62670.4196307089060542No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACACGGAGAATGCCAGTGCCCCAGGCCTGA60870.4075781275109544No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40390.2704465347489313No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGA39840.26676380154487306No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39780.2663620488317031No Hit
CATCAGGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG37960.2541755498655468No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT37200.24908668216539354No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC35890.24031508126118212No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32900.220294404388211No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG31990.2142011549051328No Hit
GTTTATTACTGTGCGCGAGAAAACCTATTAGCCGGCGCAGTAATAACAGG31220.20904532841945125No Hit
GACTATTACTGTCAGGCCTGGGGCACTGGCATTCTCCGTGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGT30830.20643393578384633No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC30380.2034207904350714No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29310.1962562003835399No Hit
CTATTACTGTCAGGCCTGGGGCACTGGCATTCTCCGTGTGTTCGGCGGAG28520.19096645632680173No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28440.19043078604257507No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28290.1894264042596501No Hit
CCTGCACTCTGAGCAGTGGGCACAGCGGCTACGCCATCGCATGGCATCAG28080.18802026976355515No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCCTGAT28050.18781939340697013No Hit
GAGAATGCCAGTGCCCCAGGCCTGACAGTAATAGTCAGCCTCATCCTCAG27970.18728372312274347No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC27900.18681501162404515No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC27850.1864802176964035No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26530.17764165800666373No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25760.1724858315209822No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG24980.16726304624977234No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24670.16518732389839405No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24370.16317856033254413No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCGGCTACGCCATCGCATGGCATC24340.16297768397595913No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24220.16217417854961913No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23720.15882623927320255No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23680.15855840413108924No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23600.15802273384686258No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23580.1578888162758059No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23460.15708531084946592No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22690.1519294843637844No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22330.14951896808476445No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21070.1410821611081947No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGGC20760.1390064387568164No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC20740.13887252118575974No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20440.1368637576199098No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA20150.1349219528395882No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20100.13458715891194653No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20090.1345202001264182No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20000.1339175710566632No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT19910.13331494198690821No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19880.13311406563032321No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT19450.13023483785260498No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19420.13003396149601998No Hit
GTAATAACAGGTGCGCCCAGTGGCTACTGGGGCCAGGGAACCCCGGTCACCGTCTCCTCAGGGAGTGCATCCGCC19110.1279582391446417No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT18720.12534684650903677No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC18470.12367287687082847No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18170.12166411330497852No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC18090.12112844302075186No Hit
GGCATTCTCCGTGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT18080.12106148423522353No Hit
CCCCTGATGTGGCTGCCATCACTGGTAAGTGTCATCAAGTATCGAGGGCC18010.12059277273652522No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG17860.11958839095360023No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACACGGAGAATGCCAGTGCCCCAGGCCTGACAGTAATAGT17640.11811529767197695No Hit
TGATGACACTTACCAGTGATGGCAGCCACATCAGGGGGGACGGGATCCCT17550.11751266860222195No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACACGGAGAATGCCAG17090.1144325644679187No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC16950.11349514147052206No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16930.11336122389946539No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC16760.11222292454548377No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16520.11061591369280381No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACACGGAGAATGC16460.11021416097963382No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16420.10994632583752048No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16350.10947761433882217No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTG16340.10941065555329382No Hit
CATTATATCCATAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGGT16260.10887498526906719No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGGCCTGGGGCACTGGCATTC16060.10753580955850056No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15820.10592879870582059No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15610.10452266420972563No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15530.10398699392549897No Hit
GTCTTTGGTGGCTCCATGAGAAGTAGTAGTTACTATTGGGCCTGGATCCG15410.103183488499159No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15380.10298261214257401No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15320.10258085942940402No Hit
ACCCAATACAACCCGTCCCTCAAGAGTCGACTCATTATATCCATAGACACGTCCAAGAACCAGTTCTCCCTGAAG15080.10097384857672405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGAT10700.054.26627370
GTACGTC200.007764389351.0536163
GGTATCA11050.046.826241
TATCGCG451.9293166E-645.7625436
ACCGATC3700.043.29838627
ATCGCGT405.1291532E-542.94297837
GACCGAT3800.042.14199426
TCTCGTA2300.041.87934538
CACGAGA3850.040.66063721
ACGAGAC4000.039.98932322
GGATGGG11600.039.4558770
TCGTATG2450.039.43274340
CGCGTAT350.001360330539.3552639
GATCAGT4000.039.26469430
AGACCGA4100.038.21075425
GTATCAA29400.038.0939941
ATCTCGT2800.038.0352137
CGTATGC2750.037.70177541
GTATCGC557.711218E-637.40797435
GACAGGT2300.037.0015681
Produced by FastQC (version 0.11.5)