FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278198
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTACTACTGTGCGAGAGACATATCGGCAGCTTTTTCCCTGTTTGAGAG74370.5818347392188065No Hit
GTAGTAGATAGCCGTGTCCTCCGGTCTCAGGTTGTTCATTTCCAGATGCA69620.5446730475247183No Hit
GTCCTGTACAGCGTCTGGATTCAGGTTCAGTGATCATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG64660.5058684178820496No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40150.3141140887405551No Hit
GTATTACTGTCAGCAGTATGCTACTTCACCCTCAATCACCTTCGGCCAAGGGACACGACTGGAGATTAATCGAA39370.30801174778868373No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG32660.2555159685745088No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31440.2459712814446588No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC29820.23329718869846455No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT26230.20521077329177484No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA25770.20161195683297892No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25710.2011425459905273No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25400.19871725663786047No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25020.19574432130233343No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24520.19183256428190312No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23590.1845566962239027No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23370.18283552313491336No Hit
GTGTATTACTGTCAGCAGTATGCTACTTCACCCTCAATCACCTTCGGCCA23280.1821314068712359No Hit
CCCTGAGACTGTCCTGTACAGCGTCTGGATTCAGGTTCAGTGATCATGGC22940.1794714120973433No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22830.17861082555284863No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22640.1771243578850851No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG22380.17509024423446135No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22370.17501200909405273No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21620.16914437356340722No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21450.16781437617646092No Hit
GTATGCTACTTCACCCTCAATCACCTTCGGCCAAGGGACACGACTGGAGATTAATCGAACTGTGGCTGCACCAT21430.1676579058956437No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21130.16531085168338552No Hit
CTCCAGAGACAATGCCAAGAACACCCTGCATCTGGAAATGAACAACCTGAGACCGGAGGACACGGCTATCTACTA20470.1601473324164175No Hit
CCTCTATGGTGCATCCAACAGGGGCAATGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA20440.15991262699519168No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCATTGCCCCTGTT20200.15803498362538512No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG20190.15795674848497648No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20090.15717439708089045No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19810.15498381314944945No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19480.15240205351596545No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19470.15232381837555684No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19220.1503679398653417No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18770.14684735854695438No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATGATCAC18370.14371795293061013No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAACTGGGTTTTC18120.14176207442039496No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18110.14168383927998635No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC17620.13785031739996464No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA17360.13581620374934086No Hit
ACCTGAGACCGGAGGACACGGCTATCTACTACTGTGCGAGAGACATATCGGCAGCTTTTTCCCTGTTTGAGAG17190.13448620636239456No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC17070.13354738467749128No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16550.12947915737624374No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16330.1277579842872544No Hit
GGGCTGGAGTGGGTGGCCGTTATATGGTTTGACGGTAGTAAGAAATATTA16230.12697563288316835No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16180.1265844571811253No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16170.12650622204071668No Hit
CTGCAGGGCCGGTCAGAATATTGACAGGGGCTACTTAGTCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGTT16090.12588034091744785No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16000.1251762246537704No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15940.12470681381131873No Hit
GTAGCATACTGCTGACAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC15770.12337681642437244No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15730.12306387586273801No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTACT15730.12306387586273801No Hit
GAATATTGACAGGGGCTACTTAGTCTGGTACCAGCAGAGACCTGGCCAGG15530.12149917305456588No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15490.12118623249293145No Hit
GTGCAGCCACAGTTCGATTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATTGAGGGTGAAGTAGCATACTG15430.12071682165047982No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15170.11868270799985604No Hit
CTACTGCAGGGCCGGTCAGAATATTGACAGGGGCTACTTAGTCTGGTACC15140.11844800257863022No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG15080.1179785917361786No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15070.11790035659576999No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT15000.11735271061290975No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14990.11727447547250112No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14790.115709772664329No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG14720.11516212668146876No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14630.11445801041779129No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCTCTCAAACAGGGAAAAAG14500.11344095359247942No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14360.11234566162675891No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14190.11101566423981261No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14170.11085919395899539No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14160.11078095881858678No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTACTGC14010.10960743171245768No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT13880.10859037488714582No Hit
CAGTAGTAGATAGCCGTGTCCTCCGGTCTCAGGTTGTTCATTTCCAGATG13750.10757331806183393No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13680.10702567207897368No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGC13620.10655626123652204No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAACTGGG13550.10600861525366179No Hit
CTCTACTGCAGGGCCGGTCAGAATATTGACAGGGGCTACTTAGTCTGGTA13550.10600861525366179No Hit
CTACTACTGTGCGAGAGACATATCGGCAGCTTTTTCCCTGTTTGAGAGCTGGGGCCAGGGAACCCTGGTCACCGT13190.10319215019895198No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG13040.10201862309282285No Hit
ATGCTACTTCACCCTCAATCACCTTCGGCCAAGGGACACGACTGGAGATTAATCGAACTGTGGCTGCACCAT13040.10201862309282285No Hit
ATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGA12790.10006274458260771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)