FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278198
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTACTACTGTGCGAGAGACATATCGGCAGCTTTTTCCCTGTTTGAGAG69170.5411524662063312No Hit
GTCCTGTACAGCGTCTGGATTCAGGTTCAGTGATCATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG63610.49765372813914593No Hit
GTAGTAGATAGCCGTGTCCTCCGGTCTCAGGTTGTTCATTTCCAGATGCA63180.4942896171015758No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39200.30668175040173745No Hit
GTATTACTGTCAGCAGTATGCTACTTCACCCTCAATCACCTTCGGCCAAGGGACACGACTGGAGATTAATCGAA37130.2904870763371559No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC28030.219293098565324No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC27260.2132689927538613No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27190.21272134677100107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26840.20998311685669985No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT26450.20693194638076418No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24760.19371020765170968No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24260.18979845063127934No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA24160.18901609922719328No Hit
GTGTATTACTGTCAGCAGTATGCTACTTCACCCTCAATCACCTTCGGCCA23890.18690375043616092No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23650.18502610706635436No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23210.18158376088837566No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23130.1809578797651068No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22880.17900200125489166No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21820.17070907637157937No Hit
CCCTGAGACTGTCCTGTACAGCGTCTGGATTCAGGTTCAGTGATCATGGC20600.16116438924172938No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG20580.16100791896091216No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20310.1588955701698798No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20050.156861456519256No Hit
GTATGCTACTTCACCCTCAATCACCTTCGGCCAAGGGACACGACTGGAGATTAATCGAACTGTGGCTGCACCAT19930.15592263483435273No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19750.1545144023069978No Hit
CCTCTATGGTGCATCCAACAGGGGCAATGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA19500.15255852379678264No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19140.14974205874207283No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18900.14786441537226627No Hit
CTCCAGAGACAATGCCAAGAACACCCTGCATCTGGAAATGAACAACCTGAGACCGGAGGACACGGCTATCTACTA18790.1470038288277716No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCATTGCCCCTGTT18720.14645618284491135No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATGATCAC18100.14160560413957773No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18060.14129266357794334No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17870.1398061959101798No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17750.13886737422527654No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA17510.13698973085546998No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17300.13534679290688922No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC16730.13088738990359866No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16730.13088738990359866No Hit
GTGCAGCCACAGTTCGATTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATTGAGGGTGAAGTAGCATACTG16640.1301832736399212No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16580.12971386279746958No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16510.12916621681460932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16490.1290097465337921No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16270.12728857344480277No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16120.12611504633867368No Hit
GGGCTGGAGTGGGTGGCCGTTATATGGTTTGACGGTAGTAAGAAATATTA16070.12572387063663062No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG16030.1254109300749962No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15910.12447210839009293No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15800.12361152184559826No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAACTGGGTTTTC15800.12361152184559826No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15760.12329858128396383No Hit
CTGCAGGGCCGGTCAGAATATTGACAGGGGCTACTTAGTCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGTT15510.12134270277374867No Hit
GAATATTGACAGGGGCTACTTAGTCTGGTACCAGCAGAGACCTGGCCAGG15500.12126446763334006No Hit
ACCTGAGACCGGAGGACACGGCTATCTACTACTGTGCGAGAGACATATCGGCAGCTTTTTCCCTGTTTGAGAG15300.11969976482516793No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG15250.11930858912312489No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15220.11907388370189909No Hit
GTAGCATACTGCTGACAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC15080.1179785917361786No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15070.11790035659576999No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14950.11696153491086671No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT14810.11586624294514622No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCTCTCAAACAGGGAAAAAG14780.11563153752392039No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14660.11469271583901712No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTACT14270.11164154536308148No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13990.10945096143164049No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13860.1084339046063286No Hit
CTACTGCAGGGCCGGTCAGAATATTGACAGGGGCTACTTAGTCTGGTACC13860.1084339046063286No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13790.10788625862346835No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGC13470.10538273413039294No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG13310.10413097188385523No Hit
CAGTAGTAGATAGCCGTGTCCTCCGGTCTCAGGTTGTTCATTTCCAGATG13230.1035050907605864No Hit
CACTTGCACTGCTGCCTACCCCGAGTCCAAGACCCCGCTAACCGCCACCC13170.10303567991813475No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13020.10186215281200565No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12920.1010798014079196No Hit
ATGCTACTTCACCCTCAATCACCTTCGGCCAAGGGACACGACTGGAGATTAATCGAACTGTGGCTGCACCAT12850.10053215542505933No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12800.10014097972301632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA601.1277603E-555.47717770
GTCACCG19350.053.67094470
AACCGTT200.007652694451.2406657
TCTGGAG19200.047.84906470
GGTATCA9500.044.9461941
CCGCTTA558.564328E-539.3652369
GTATCAA24500.037.923581
AATGGTA5850.036.41578770
AGTCGAC400.002717630734.1805112
GTGCTAG1550.033.05721
CTCTGGA19800.032.14827369
ATCAACG28700.031.8877183
TCAACGC28700.031.8877184
TATCAAC29150.031.400372
CAACGCA29550.030.9704745
AACGCAG29900.030.8387746
CGGTCTC16700.030.71789722
TGAGAGC20150.030.50600245
GGTCACC22300.030.48599269
TTTTCCC20250.030.40558434
Produced by FastQC (version 0.11.5)