FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058659
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49520.4677615738401128No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44510.42043755354651496No Hit
CTCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG43640.41221960990271656No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35570.33599109817231043No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32470.30670877024613213No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA31110.2938623296075507No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29990.2832829079051895No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28890.2728924044475133No Hit
GTAATAGACCGCCGCGTCGTCGGGAGTCACAGACTTCAATTGCAAAGAGA28300.26731931622930516No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28080.26524121553776997No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTCACGGACACAATTGCTTGAATTGGTACCTGCAGAAGACAGGGCAGTCTC28030.2647689199260574No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27480.25957366819721933No Hit
GCAGAAGACAGGGCAGTCTCCACAACTCCTGATCTATTCGGGTTCCAATCGGGCCTCCGGGGTCCCTGACAGGTT27000.2550396303247788No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26380.24918316473954313No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26000.24559371809052774No Hit
GTTTATTACTGCATGGAAACTCTACAACTTCCGTACACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAA25760.2433266991543075No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG25410.24002062987231962No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25360.23954833426060707No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT25100.23709239707970178No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGGAAACTCTACAACTT24270.22925228992527338No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24210.22868553519121834No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24010.22679635274436813No Hit
CTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA24000.22670189362202559No Hit
CTCTACAACTTCCGTACACTTTTGGCCGGGGGACCAAGCTGGAGATCAAA23630.2232069060953527No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23580.22273461048364016No Hit
GTCTTCTGCAGGTACCAATTCAAGCAATTGTGTCCGTGACTATGCAGGAG23240.2195230003239948No Hit
GTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAAAATCAGCAGAG23090.21810611348885714No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC22850.21583909455263686No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22050.20828236476523604No Hit
GTGTAAAATCTGTGCCTGATGCACTGCCACTGAACCTGTCAGGGACCCCG20990.19826969779692988No Hit
GTCCCTGACAGGTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAA20840.19685281096179222No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCCGGCCAAAAGTG20480.19345228255746183No Hit
GTCTATTACTGCGCAAGAGGTATAGAGTCCCACTACTTAGACTCCTGGGGCCAGGGAAACCTGGTCTCCGTCTC20480.19345228255746183No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20440.1930744460680918No Hit
CTCCAGGAGTGACGGACAGGGAGAGTGGAGACTGAGTCATCACAATATCC19310.1824005652433881No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA19220.18155043314230548No Hit
CCCATGGGGTGTCCTGTCACAGGTACAACTGCAGCAGTCAGGTCCAGGAC19150.18088921928590793No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18870.17824436386031764No Hit
GTTGTAGAGTTTCCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT18410.17389924423256214No Hit
GTCAGGGACCCCGGAGGCCCGATTGGAACCCGAATAGATCAGGAGTTGTGGAGACTGCCCTGTCTTCTGCAGGTA18380.1736158668655346No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGTCCGTCACTC18310.17295465300913704No Hit
GTCTCCACTCTCCCTGTCCGTCACTCCTGGAGAGTCGGCCTCCATCTCCT18220.17210452090805445No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC18120.17115992968462931No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18000.1700264202165192No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17810.1682316968920115No Hit
CTCCTGATCTATTCGGGTTCCAATCGGGCCTCCGGGGTCCCTGACAGGTT17610.1663425144451613No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17560.16587021883344874No Hit
GGCCAAAAGTGTACGGAAGTTGTAGAGTTTCCATGCAGTAATAAACTCCA17300.16341428165254346No Hit
GTCACGGACACAATTGCTTGAATTGGTACCTGCAGAAGACAGGGCAGTCTCCACAACTCCTGATCTATTCGGGTT17200.16246969042911835No Hit
CTCCTGCATAGTCACGGACACAATTGCTTGAATTGGTACCTGCAGAAGACAGGGCAGTCTCCACAACTCCTGAT16970.16029713061524062No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT16600.1568021430885677No Hit
CCACTCTCCCTGTCCGTCACTCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGT16570.15651876572154017No Hit
AGACAGGGCAGTCTCCACAACTCCTGATCTATTCGGGTTCCAATCGGGCC16440.15529079713108754No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16380.15472404239703247No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16340.15434620590766243No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16190.15292931907252477No Hit
GATCAGGAGTTGTGGAGACTGCCCTGTCTTCTGCAGGTACCAATTCAAGC16070.15179580960441463No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16010.15122905487035956No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT15610.14745068997665917No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14950.14121638790205343No Hit
CTCCACAACTCCTGATCTATTCGGGTTCCAATCGGGCCTCCGGGGTCCCT14920.1409330105350259No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14680.13866599159880566No Hit
GCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTGGAACCCGAATAGATCAGGAGTTGTGGAGACTGCC14650.13838261423177814No Hit
AGTCAGAGCCTCCTGCATAGTCACGGACACAATTGCTTGAATTGGTACCTGCAGAAGACAGGGCAGTCTCCACAA14520.13715464564132548No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14030.13252614864654247No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14010.13233723040185744No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13940.13167601654545988No Hit
GCCCTGTCTTCTGCAGGTACCAATTCAAGCAATTGTGTCCGTGACTATGC13880.1311092618114048No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13850.1308258844443773No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13780.1301646705879797No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13710.12950345673158212No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13370.12629184657193676No Hit
GTTGTGGAGACTGCCCTGTCTTCTGCAGGTACCAATTCAAGCAATTGTGTCCGTGACTATGCAGGAGGCTCTGA13340.12600846920490924No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13340.12600846920490924No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13240.12506387798148413No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13230.12496941885914162No Hit
GACTAGACCTGCAGGAGATGGAGGCCGACTCTCCAGGAGTGACGGACAGG13230.12496941885914162No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGGAAACTCTACAACTTCCGTACACTTTTGGCCGGGGGA13160.12430820500274405No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13040.12317469553463391No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12980.12260794080057884No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12810.12100213572075616No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12730.12024646274201609No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12700.11996308537498854No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGTCCGTCACTCCTGGAGAGTCGGCCTCCATCTCCTGCAGGT12690.11986862625264605No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGACTCTCCAGGAGTGACGGACAGGGAGAGTGGAGA12660.1195852488856185No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12480.1178849846834533No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12240.11561796574723306No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12200.115240129257863No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12120.11448445627912293No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11870.11212297822056015No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11820.1116506826088476No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11580.10938366367262735No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11530.10891136806091481No Hit
CCACAACTCCTGATCTATTCGGGTTCCAATCGGGCCTCCGGGGTCCCTGA11530.10891136806091481No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11490.10853353157154474No Hit
GTCAATTACCTGTGCCATCTCCGGGGACAGTGTCTCCACCCACCTTGCTA11430.1079667768374897No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11290.10664434912469455No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGGAAACTCTACAACT11250.10626651263532451No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11180.10560529877892692No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC11080.10466070755550182No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11080.10466070755550182No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11070.1045662484331593No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10960.10352719808739169No Hit
CTATACCTCTTGCGCAGTAATAGACCGCCGCGTCGTCGGGAGTCACAGACTTCAATTGCAAAGAGAACTGGTT10900.10296044335333662No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10890.1028659842309941No Hit
GGATCAGGCAGTCCCCATCGAGGGGCCTTGAGTGGCTGGGAAGGACATAC10820.10220477037459655No Hit
CAATTGCTTGAATTGGTACCTGCAGAAGACAGGGCAGTCTCCACAACTCCTGATCTATTCGGGTTCCAATCGGGC10680.10088234266180139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTTG7800.057.5115470
ATTTACG200.007807720550.9807527
CTAACGC200.00788399250.855543
GGTATCA10000.046.4612271
CGTATGC2600.039.86528441
ATGCCGT2500.038.85111644
TATCTCG2400.037.1394836
TCGTATG2700.037.05031640
ACGAGAC2900.035.09267822
CTGGGTT8900.034.95081369
GCCGTCT2800.034.81974446
GTATCAA22200.034.6771161
GTATGCC2900.034.59320442
CGAGACG2850.034.52295723
CACGAGA2850.034.51479721
ATCTCGT2650.033.67113537
AGCCCAC2950.033.3306317
TCCTATC2900.032.9995733
CCCACGA3000.032.77821719
TCTCCGA3150.032.29228211
Produced by FastQC (version 0.11.5)